全文获取类型
收费全文 | 4416篇 |
免费 | 329篇 |
国内免费 | 210篇 |
出版年
2023年 | 27篇 |
2022年 | 57篇 |
2021年 | 114篇 |
2020年 | 115篇 |
2019年 | 155篇 |
2018年 | 206篇 |
2017年 | 189篇 |
2016年 | 229篇 |
2015年 | 178篇 |
2014年 | 225篇 |
2013年 | 269篇 |
2012年 | 262篇 |
2011年 | 261篇 |
2010年 | 241篇 |
2009年 | 212篇 |
2008年 | 224篇 |
2007年 | 228篇 |
2006年 | 188篇 |
2005年 | 200篇 |
2004年 | 192篇 |
2003年 | 200篇 |
2002年 | 196篇 |
2001年 | 137篇 |
2000年 | 97篇 |
1999年 | 51篇 |
1998年 | 36篇 |
1997年 | 44篇 |
1996年 | 23篇 |
1995年 | 37篇 |
1994年 | 21篇 |
1993年 | 20篇 |
1992年 | 24篇 |
1991年 | 20篇 |
1990年 | 22篇 |
1989年 | 28篇 |
1988年 | 13篇 |
1986年 | 8篇 |
1985年 | 15篇 |
1984年 | 20篇 |
1983年 | 13篇 |
1982年 | 13篇 |
1980年 | 15篇 |
1979年 | 11篇 |
1978年 | 16篇 |
1977年 | 10篇 |
1976年 | 12篇 |
1974年 | 10篇 |
1973年 | 14篇 |
1972年 | 11篇 |
1970年 | 9篇 |
排序方式: 共有4955条查询结果,搜索用时 687 毫秒
1.
Application of 1% methol, which, along with cold, activates specific thermosensitive ionic channels, changes the number of functioning cold receptors on the skin of the forearm similarly to the cold exposure test; however, it does not affect the number of heat receptors and does not significantly change the threshold of cold sensation. Group variants of responses to menthol that indicate individual differences in the sensitivity of skin receptors to the effects of methol and cold have been found. The results obtained give grounds to suggest that, from the variant of response to menthol (a decrease, increase, or absence of changes in the number of functioning cold receptors 5 min after menthol application), it is possible to predict specific features of response to cold. 相似文献
2.
E. P. Isakova Yu. I. Deryabina N. N. Gessler T. A. Belozerskaya Ya. M. Rabinovich 《Applied Biochemistry and Microbiology》2010,46(3):318-323
Cell respiratory activity of protoplasts obtained from the wild type of Neurospora crassa and photoreceptor complex WCC—white collar 1 (wc-1) and white collar 2 (wc-2)—mutants of Neurospora crassa strains was investigated. Respiration inhibition by KCN in the presence of 25 mM succinate was similar in all strains and
did not exceed 83–85% against control. The significant induction of KCN-resistant respiratory pathway occurred under 1% glucose
oxidation in wc-1 and wc-2 mutants if compared with the wild type strains. The inhibitors of the main (cytochrome) pathway of electron transfer in mitochondria—1
mM KCN and antimycin A (4 μg/ml)—blocked the respiration rate of the protoplasts from N. crassa wild type by 75%, while the cell respiration of wc-1 and wc-2 strains was suppressed by approximately 50%. The specific inhibitor of alternative oxidase—10 mM salicylhydroxamic acid (SHAM)—in
combination with the blockers of mitochondrial electron transfer chain caused the total suppression of respiratory activity
of protoplasts in all studied strains. It is supposed that an increase of KCN-resistance in WCC mutants under glucose oxidation
is connected with alternative oxidase activation as the result of failure in reception and signal transduction of active oxygen
species. 相似文献
3.
4.
Cannibalism is known to be a significant source of natural mortality of young North-east Arctic (NEA) cod. Cannibalism data, starting from 1984, have been used in NEA cod stock assessments since 1995, which has led to inconsistency in the cod abundance time series from 1946 to the present. To address this inconsistency, this study estimates the cannibalism-induced mortality (M2) of NEA cod at age 3–5 for the period 1946–1983. Combined qualitative and quantitative cod stomach content data for 1984–2010 were used to make the M2 estimations for age groups 3–5 (ICES 2014), then different factors including SSB were used to examine which covariates explained variability in M2 and thus make predictions for 1946–1983. The level of cannibalism was estimated to be high in the 1950s – early1960s. VPA-based assessment was run using these estimated M2 values. As a result, numbers of cod eaten by their conspecifics in the historical period and new increased recruitment estimates at age 3 were computed. The main factors affecting cannibalism appeared to be young cod abundance, total stock biomass (TSB) of large cod, and capelin total stock biomass (which represents an alternative prey). The problems involved in using the new recruitment time series in fishery management are discussed. The methodology presented here represents a generic approach to extending predation mortalities back in time to improve historical stock estimates. 相似文献
5.
Ya. O. Loginov G. G. Khudaigulov S. P. Chetverikov A. I. Melent’ev O. N. Loginov 《Applied Biochemistry and Microbiology》2011,47(3):311-314
Highly viscous polysaccharide (250–350 kDa) of an alginate nature with a predominance of α-L-guluronic acid (M/G = 0.22) was
obtained from Azotobacter vinelandi. The yield of polysaccharide was 20.5 ± 0.5 g/l when cultured in a medium containing molasses at a viscosity of the cultural
liquid of over 30000 cSt. The biopolymer is stable at pH 4.0–9.0 in a wide temperature range and well soluble in highly mineralized
water; it retains a high viscosity level and can be used in the petroleum industry for enhanced oil recovery. 相似文献
6.
K W Culver W T Hsieh Y Huyen V Chen J Liu Y Khripine A Khorlin 《Nature biotechnology》1999,17(10):989-993
A sequence-specific genomic delivery system for the correction of chromosomal mutations was designed by incorporating two different binding domains into a single-stranded oligonucleotide. A repair domain (RD) contained the native sequence of the target region. A third strand-forming domain (TFD) was designed to form a triplex by Hoogsteen interactions. The design was based upon the premise that the RD will rapidly form a heteroduplex that is anchored synergistically by the TFD. Deoxyoligonucleotides were designed to form triplexes in the human adenosine deaminase (ADA) and p53 genes adjacent to known point mutations. Transfection of ADA-deficient human lymphocytes corrected the mutant sequence in 1-2% of cells. Neither the RD or TFD individually corrected the mutation. Transfection of p53 mutant human glioblastoma cells corrected the mutation and induced apoptosis in 7.5% of cells. 相似文献
7.
I. Ya. Kleinbok 《Neurophysiology》1986,18(6):542-547
The effect of picrotoxin (at sub-convulsant doses, i.v.) on the background and invoked spike activity of cat dorsal horn internerons was investigated while thermal and mechanical influences were at work on the skin receptor areas. It was found that information on skin temperature is modulated at the presynaptic level and largely mediated by competitive interaction between large and small diameter fibers.Institute of Physiology, Academy of Sciences of the Kazakh SSR, Alma Ata. Translated from Neirofiziologiya, Vol. 18, No. 6, pp. 773–779, November–December, 1986. 相似文献
8.
9.
A. Ya. Ivanov 《Neurophysiology》1987,19(5):495-501
Tonic activity in rabbit superior cervical ganglion neurons was investigated using intracellular recording techniques as well as changes produced when the animal breathed a gaseous mix with a raised CO2 level. The test neurons were divided into three groups depending on the pattern of their tonic activity and reflex change. Action potentials were produced by the activity of dominant and accessory preganglionic inputs in the firing pattern of all neuronal groups, implying the existence of other types of inputs into the neurons innervating different organs. Having analyzed changes in action potential rate and EPSP in the tonic activity of neurons from different groups, it was presumed that preganglionic fibers with a similar activity pattern converge on the majority of neurons in each group.A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 19, No. 5, pp. 665–672, September–October, 1987. 相似文献
10.