全文获取类型
收费全文 | 20948篇 |
免费 | 1589篇 |
国内免费 | 1456篇 |
出版年
2024年 | 40篇 |
2023年 | 262篇 |
2022年 | 611篇 |
2021年 | 1113篇 |
2020年 | 771篇 |
2019年 | 953篇 |
2018年 | 880篇 |
2017年 | 664篇 |
2016年 | 964篇 |
2015年 | 1350篇 |
2014年 | 1586篇 |
2013年 | 1631篇 |
2012年 | 1909篇 |
2011年 | 1649篇 |
2010年 | 1031篇 |
2009年 | 881篇 |
2008年 | 1026篇 |
2007年 | 847篇 |
2006年 | 796篇 |
2005年 | 633篇 |
2004年 | 611篇 |
2003年 | 542篇 |
2002年 | 471篇 |
2001年 | 423篇 |
2000年 | 382篇 |
1999年 | 341篇 |
1998年 | 222篇 |
1997年 | 202篇 |
1996年 | 185篇 |
1995年 | 161篇 |
1994年 | 125篇 |
1993年 | 110篇 |
1992年 | 135篇 |
1991年 | 118篇 |
1990年 | 99篇 |
1989年 | 62篇 |
1988年 | 50篇 |
1987年 | 51篇 |
1986年 | 27篇 |
1985年 | 28篇 |
1984年 | 16篇 |
1983年 | 20篇 |
1982年 | 4篇 |
1980年 | 5篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 906 毫秒
1.
2.
3.
Yunfan Sun Liang Wu Yu Zhong Kaiqian Zhou Yong Hou Zifei Wang Zefan Zhang Jiarui Xie Chunqing Wang Dandan Chen Yaling Huang Xiaochan Wei Yinghong Shi Zhikun Zhao Yuehua Li Ziwei Guo Qichao Yu Liqin Xu Jia Fan 《Cell》2021,184(2):404-421.e16
- Download : Download high-res image (233KB)
- Download : Download full-size image
4.
Plant and Soil - Seeds are involved in the transmission of microorganisms from one plant generation to the next, acting as initial inoculum for the plant microbiome, therefore provide a key source... 相似文献
5.
6.
Ai‐Xin Song Chen‐Jie Zhou Xiao Guan Kong‐Hung Sze Hong‐Yu Hu 《Protein science : a publication of the Protein Society》2010,19(5):1104-1109
DC‐UbP/UBTD2 is a ubiquitin (Ub) domain‐containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. The solution structure and backbone dynamics of the C‐terminal Ub‐like (UbL) domain were elucidated in our previous work. To further understand the biological function of DC‐UbP, we then solved the solution structure of the N‐terminal domain of DC‐UbP (DC‐UbP_N) and studied its Ub binding properties by NMR techniques. The results show that DC‐UbP_N holds a novel structural fold and acts as a Ub‐binding domain (UBD) but with low affinity. This implies that the DC‐UbP protein, composing of a combination of both UbL and UBD domains, might play an important role in regulating protein ubiquitination and delivery of ubiquitinated substrates in eukaryotic cells. 相似文献
7.
A growing body of literature has shown that stem cells are very effective for the treatment of degenerative diseases in rodents but these exciting results have not translated to clinical practice. The difference results from the divergence in genetic, metabolic, and physiological phenotypes between rodents and humans. The high degree of similarity between non-human primates(NHPs) and humans provides the most accurate models for preclinical studies of stem cell therapy. Using a NHP model to understand the following key issues, which cannot be addressed in humans or rodents, will be helpful for extending stem cell applications in the basic science and the clinic. These issues include pluripotency of primate stem cells, the safety and efficiency of stem cell therapy, and transplantation procedures of stem cells suitable for clinical translation. Here we review studies of the above issues in NHPs and current challenges of stem cell applications in both basic science and clinical therapies. We propose that the use of NHP models, in particular combining the serial production and transplantation procedures of stem cells is the most useful for preclinical studies designed to overcome these challenges. 相似文献
8.
Effect of fertilizers on Cd accumulation and subcellular distribution of two cosmos species (Cosmos sulphureus and Cosmos bipinnata) 总被引:1,自引:0,他引:1
Addition of fertilizer amendments is regarded as an ideal approach to enhancing phytoextraction. However, there is little information available on the influence of common fertilizers on Cd accumulation of two newly reported Cd accumulators, Cosmos sulphureus and Cosmos bipinnata (C. sulphureus and C. bipinnata). The effects of N (CO(NH2)2), NP (CO(NH2)2 + Ca(H2PO4)2), and NPK (CO(NH2)2 + Ca(H2PO4)2 + KCl) fertilizers on Cd accumulation and subcellular distribution of C. sulphureus and C. bipinnata were studied in a 70-d pot experiment. The results showed that Cd uptake of C. sulphureus and C. bipinnata with NPK fertilizer was significantly higher than control, N, and NP fertilizers, especially 3.8- and 4.7-fold higher than control (p < 0.05). Compared with C. bipinnata, C. sulphureus achieved higher biomass and Cd uptake in aboveground parts under fertilizer treatments, especially NPK fertilizer. The Cd subcellular distribution revealed that segregation of Cd to Cd-rich granules (MRG) might play an important role in Cd detoxification in both species. C. sulphureus is more likely than C. bipinnata to separate the Cd in MRG and reduce the partition in the heat-denatured protein fraction, especially with NPK fertilizer. Therefore, C. sulphureus combined with NPK fertilizers could be an effective method to remediate Cd-polluted farmland soils in China. 相似文献
9.
Many animals exhibit different behaviors in different seasons. The photoperiod can have effects on migration, breeding, fur growth, and other processes. The cyclic growth of the fur and feathers of some species of mammals and birds, respectively, is stimulated by the photoperiod as a result of hormone-dependent regulation of the nervous system. To further examine this phenomenon, we evaluated the Arbas Cashmere goat (Capra hircus), a species that is often used in this type of research. The goats were exposed to an experimentally controlled short photoperiod to study the regulation of cyclic cashmere growth. Exposure to a short photoperiod extended the anagen phase of the Cashmere goat hair follicle to increase cashmere production. Assessments of tissue sections indicated that the short photoperiod significantly induced cashmere growth. This conclusion was supported by a comparison of the differences in gene expression between the short photoperiod and natural conditions using gene chip technology. Using the gene chip data, we identified genes that showed altered expression under the short photoperiod compared to natural conditions, and these genes were found to be involved in the biological processes of hair follicle growth, structural composition of the hair follicle, and the morphogenesis of the surrounding skin appendages. Knowledge about differences in the expression of these genes as well as their functions and periodic regulation patterns increases our understanding of Cashmere goat hair follicle growth. This study also provides preliminary data that may be useful for the development of an artificial method to improve cashmere production by controlling the light cycle, which has practical significance for livestock breeding. 相似文献
10.
Jun‐juan Wang Qi‐min Zhang Wei‐wei Ni Xin Zhang Ying Li Ai‐li Li Peng Du Chun Li Su‐su Yu 《Microbiology and immunology》2019,63(8):303-315
We investigated the correlation between the beneficial effect of Lactobacillus acidophilus on gut microbiota composition, metabolic activities, and reducing cow's milk protein allergy. Mice sensitized with β‐lactoglobulin (β‐Lg) were treated with different doses of L. acidophilus KLDS 1.0738 for 4 weeks, starting 1 week before allergen induction. The results showed that intake of L. acidophilus significantly suppressed the hypersensitivity responses, together with increased fecal microbiota diversity and short‐chain fatty acids (SCFAs) concentration (including propionate, butyrate, isobutyrate, and isovalerate) when compared with the allergic group. Moreover, treatment with L. acidophilus induced the expression of SCFAs receptors, G‐protein–coupled receptors 41 (GPR41) and 43 (GPR43), in the spleen and colon of the allergic mice. Further analysis revealed that the GPR41 and GPR43 messenger RNA expression both positively correlated with the serum concentrations of transforming growth factor‐β and IFN‐γ (p < .05), but negatively with the serum concentrations of IL‐17, IL‐4, and IL‐6 in the L. acidophilus–treated group compared with the allergic group (p < .05). These results suggested that L. acidophilus protected against the development of allergic inflammation by improving the intestinal flora, as well as upregulating SCFAs and their receptors GPR41/43. 相似文献