金线蛙染色体G显带的方法学探索

采用自然老化、氯仿处理及EDTA显带法,首次在金线蛙（R.plancyt）有丝分裂中期染色体上成功显示出G带,带纹反差强烈、特征鲜明。比较了多个来自同一分裂相的同源染色体和不同分裂相的4条大染色体,发现其特征带纹高度一致,带纹数目基本吻合。该方法经济简便,结果稳定可靠。探讨了G显带的可能机制,对进一步开展金线蛙等两栖类的细胞遗传学研究具有一定的参考价值。 Explore on the Advanced Method of G-banding of Rana Plancyt QIAN Shui-ming,YU Qi-xing College of Life Sciences,Wuhan University,Wuhan 430072,China Abstract:Using the technique of natural aging,chloroform and EDTA treatment,well-resolved G-banding patterns were successfully obtained in Rana plancyt's mitosis chromosome firstly.The G-banding patterns appeared distinctive.When analyzing some homologous chromosomes from the same metaphase figures and four macrochromosomes from different metaphase figures,the characteristic and the number of the bands were well matched.The method in this study is also economic and simple and the result is stable and reliable.The possible mechanism of G-banding was primarily discussed.The application of this method in the cytogenetics research of Rana plancyt and other amphibians is expected. Key words：Rana plancyt; mitosis; G-banding