芽殖酵母PHO85基因对细胞周期调控的影响

本文通过同源重组方法,构建了一系列单倍体缺失株:pho85△单缺失株YPH600、pho85△cln1△双缺失株YPH610、cln1△cln2△双缺失株YPH640和半乳糖诱导存活的pho85△chn1△cln2△(GAL1-10PHO85)三缺失株YPH630。比较不同缺失株的生长速度可以看出,PHO85单基因缺失比CLN1、CLN2双基因缺失对细胞生长速度的影响大。在去诱导的不同时间,取细胞进行光学显微镜形态学分析及DNA含量的流式细胞计量分析(FACS)。结果表明YPH630三缺失株的单倍体酵母细胞休止在G1期。     

芽残酵母PHO85基因对细胞周期调控的影响

By homo-recombination with yeast intergrating plasmids, a serial of haploid mutants of budding yeast YPH499 had been constructed, which included pho85 delta strain YPH600, pho85 delta cln1 delta strain YPH610, cln1 delta cln2 delta strain YPH640 and galactose inducible strain YPH630 (pho85 delta cln1 delta cln2 delta (GAL1-10PHO85)). By analyzing the growth rate of different strains, we concluded that PHO85 gene have greater influence than CLN1 and CLN2 genes on cell growth control. After transferred from galactose media to glucose media, the tri-mutant cells collected at intervals were observed with microscope and analyzed by FACS. The results showed that the tri-mutant cells arrest in G1 phase when they were transferred to glucose media.     

468例甲真菌病临床类型及病原菌分析

目的探讨本地区甲真菌病的菌种构成、临床分型和各年龄段发病情况。方法对2010年1月至2013年12月来平湖市中医院皮肤科就诊的468例甲屑培养阳性病例病原菌进行回顾性统计分析。结果甲真菌病以皮肤癣菌为主,占58.76%;酵母菌次之,占30.13%;霉菌第三,占11.11%。临床分型远端甲下型(DLSO)、全甲营养不良型(TDO)、近端甲下型(PSO)和浅表白色型(SWO)分别为50.64%、25.21%、16.03%和8.12%。发病年龄以36~50岁为主,20~35岁次之。结论甲真菌病病原菌以皮肤癣菌为主,临床分型以DLSO型为主,年龄分布以20~50岁为主。