野猪及其与家猪杂种猪Myostatin基因3'编码区的克隆和序列分析

Myostatin, which is a highly conservative gene among breeds, is a negative regulator of muscle. The 3' coding region of wild boar and crossbred pig myostatin was cloned by RT - PCR and sequenced. Compared with that of GenBank, the homology of the nucleotide sequence between wild bear and crossbred pig is identical in this region indicating that domestic pigs were evolved from wild boar and there was not changed in this region during the evolution processes.     

杂种猪染色体的核型与显带研究

应用常规方法获得了杂种猪( 野猪（♂）×家猪（♀）)的核型、C带和174条带纹的G带,应用微量秋水仙素法得到了258条带纹的高分辨G带。结果表明,杂种猪的二倍体细胞染色体数目2n=38,C带具有多态性,G带和高分辨G带与家猪相比无明显差异,它们属于同一种。 Abstract:Using usual method,we got karyotype of hybrid pig (wild soar（♂）×domestic pig（♀）),C-band and approximate 174 bands of G-band,and we also obtained approximate 258 bands of high resolution G-band by micro-Colchicin method.The result indicate that the diploid chromosome number is 2n=38;there is polymorphism in C-band,and compared with domestic pig in G-band and high resolution G-band there is no distinguish difference.They belong to the same seed.