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Branching patterns of rabbit oculomotor and trochlear nerves demonstrated by Sihler's stain technique 总被引:1,自引:0,他引:1
R. G zil D. Kadioglu E. Calgü ner D. Erdogan M. Bahcelioglu C. Elmas 《Biotechnic & histochemistry》2002,77(1):21-25
A modified Sihler's stain technique was used to visualize the branching patterns of oculomotor and trochlear nerves. The levator palpebrae, superior rectus, inferior rectus, medial rectus, inferior oblique, superior oblique and tensor trochlea muscles were isolated from the eyes of normal rabbits and processed using modified Sihler's technique. The distributions and terminal ramifications of the oculomotor and trochlear nerves were observed. Two distinct divisions and terminal branches of the oculomotor nerve were shown in detail together with the trochlear nerve distribution. The application of Sihler's technique enables researchers to trace nerve branching within relatively transparent muscles, whereas the nerve fibers are counterstained and clearly visible. This technique could be useful for detailed studies of the motor control of extraocular muscles. 相似文献
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Ivana Vančurová Aleš Vančura Jindřich Volc Jiří Neužzil Miroslav Flieger Gabriela Basařová Vladislav Běhal 《Archives of microbiology》1988,150(5):438-440
Alanine dehydrogenase was purified to near homogeneity from cell-free extract of Streptomyces aureofaciens, which produces tetracycline. The molecular weight of the enzyme determined by size-exclusion high-performance liquid chromatography was 395 000. The molecular weight determined by sodium dodecyl sulfate gel electrophoresis was 48 000, indicating that the enzyme consists of eight subunits with similar molecular weight. The isoelectric point of alanine dehydrogenase is 6.7. The pH optimum is 10.0 for oxidative deamination of L-alanine and 8.5 for reductive amination of pyruvate. K
M values were 5.0 mM for L-alanine and 0.11 mM for NAD+. K
M values for reductive amination were 0.56 mM for pyruvate, 0.029 mM for NADH and 6.67 mM for NH4Cl.Abbreviation AlaDH
alanine dehydrogenase 相似文献
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