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The metabolism of glucose to glycogen in the liver of fasted and well-fed rats was investigated with 13C nuclear magnetic resonance spectroscopy using [1,2-(13)C2]glucose as the main substrate. The unique spectroscopic feature of this molecule is the 13C-13C homonuclear coupling leading to characteristic doublets for the C-1 and C-2 resonances of glucose and its breakdown products as long as the two 13C nuclei remain bonded together. The doublet resonances of [1,2-(13)C2]glucose thus provide an ideal marker to follow the fate of this exogenous substrate through the metabolic pathways. [1,2-(13)C2]Glucose was injected intraperitoneally into anesthetized rats and the in vivo 13C-NMR measurements of the intact animals revealed the transformation of the injected glucose into liver glycogen. Glycogen was extracted from the liver and high resolution 13C-NMR spectra were obtained before and after hydrolysis of glycogen. Intact [1,2-13C2]glucose molecules give rise to doublet resonances, natural abundance [13C]glucose molecules produce singlet resonances. From an analysis of the doublet-to-singlet intensities the following conclusions were derived. (i) In fasted rats virtually 100% of the glycosyl units in glycogen were 13C-NMR visible. In contrast, the 13C-NMR visibility of glycogen decreased to 30-40% in well-fed rats. (ii) In fed rats a minimum of 67 +/- 7% of the exogenous [1,2-(13)C2]glucose was incorporated into the liver glycogen via the direct pathway. No contribution of the indirect pathway could be detected. (iii) In fasted rats externally supplied glucose appeared to be consumed in different metabolic processes and less [1,2-(13)C2]glucose was found to be incorporated into glycogen (13 +/- 1%). However, the observation of [5,6-(13)C2]glucose in liver glycogen provided evidence for the operation of the so-called indirect pathway of glycogen synthesis. The activity of the indirect pathway was at least 9% but not more than 30% of the direct pathway. (vi) The pentose phosphate pathway was of little significance for glucose but became detectable upon injection of [1-(13)C]ribose. 相似文献
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Cerebral metabolism of [1,2-13C2]acetate as detected by in vivo and in vitro 13C NMR 总被引:3,自引:0,他引:3
The metabolism of [1,2-13C2]acetate in rat brain was studied by in vivo and in vitro 13C NMR spectroscopy, in particular by taking advantage of the homonuclear 13C-13C spin coupling patterns. Well nourished rats were infused with [1,2-13C2]acetate or [1-13C]acetate in the jugular vein, and the in situ kinetics of 13C labeling during the infusion period was followed by 13C NMR techniques. The in vivo 13C NMR spectra showed signals from (i) the C-1 carbon of [1,2-13C2] acetate or [1-13C]acetate, (ii) 13CO3H-, and (iii) the natural abundance 13C carbons of sufficiently mobile fatty acids. Methanol/HCl/perchloric acid extracts of the brains were prepared and were further analyzed by high resolution 13C NMR. The homonuclear 13C-13C spin coupling patterns after infusion of [1,2-13C2]acetate showed very different isotopomer populations in glutamate, glutamine, and gamma-aminobutyric acid. Analyzing the relative proportions of these isotopomers revealed (i) two different glutamate compartments in the rat brain characterized by the presence and absence, respectively, of glutamine synthase activity, (ii) two different tricarboxylic acid cycles, one preferentially metabolizing [(1,2-13C2]acetate, the other mainly using unlabeled acetyl-coenzyme A, (iii) a hitherto unknown cerebral pyruvate recycling system associated with the tricarboxylic acid cycle, metabolizing primarily unlabeled acetyl-coenzyme A, and (iv) a predominant production of gamma-aminobutyric acid in the glutamate compartment lacking glutamine synthase. 相似文献
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Lars A. Svensson Jürgen Dnnecke Karl‐Erik Karlsson Anders Karlsson Jrgen Vessman 《Chirality》1999,11(2):121-128
Vancomycin selectively immobilized to silica via either one of its two amino groups has been investigated and compared with columns made from native vancomycin. The chemical modification of vancomycin prior to immobilization involved protection of one amino group as a 9‐fluorenylmethyl carbamate. The immobilization and the subsequent cleavage of the protecting group was performed on‐column. The types of compounds that can be separated with the vancomycin chiral stationary phases resemble those separated previously by capillary electrophoresis and thin‐layer chromatography. The protected chiral stationary phases were also investigated and in some cases very high enantioselectivity were obtained. One example of this is a separation of thalidomide with an α‐value as high as 5.4. The soft immobilization procedure preserves the structure of native vancomycin, in contrast to other approaches. Good repeatability and stability of the columns have also been obtained. Chirality 11:121–128, 1999. © 1999 Wiley‐Liss, Inc. 相似文献
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Klinkowski Aschersleben Schlegel G. Könnecke H. Sagromsky M. Klinkowski E. Hoffmann Wussow Friedrich A. Bail 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1954,24(2-3):92-96
Ohne Zusammenfassung 相似文献
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Michel M. Bélombé Justin Nenwa Gouet Bebga Evamarie Hey-Hawkins Peter Lönnecke 《Inorganica chimica acta》2009,362(1):1-4
The title compound, Ag11.60H0.40[Cr(C2O4)3]4 · 15H2O (1) precipitates from aqueous solution as a dark violet solid in which silver ions are partially replaced by protons, and it crystallizes in an unusual structure with water-filled one-dimensional pseudo-nanotubes. 相似文献
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Incorporation of neutral C-terminal residues in 3-amidinophenylalanine-derived matriptase inhibitors
Andrea Schweinitz Daniel Dönnecke Alexander Ludwig Peter Steinmetzer Alexander Schulze Joscha Kotthaus Silvia Wein Bernd Clement Torsten Steinmetzer 《Bioorganic & medicinal chemistry letters》2009,19(7):1960-1965
A novel series of matriptase inhibitors based on previously identified tribasic 3-amidinophenylalanine derivatives was prepared. The C-terminal basic group was replaced by neutral residues to reduce the hydrophilicity of the inhibitors. The most potent compound 22 inhibits matriptase with a Ki value of 0.43 nM, but lacks selectivity towards factor Xa. By combination with neutral N-terminal sulfonyl residues several potent thrombin inhibitors were identified, which had reduced matriptase affinity. 相似文献
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Reaction of [NiCl2(dtbpe)] (dtbpe = 1,2-bis(di-tert-butylphosphino)ethane) with one equivalent of NaBArF4 (BArF4 = tetrakis[3,5-bis(trifluoromethyl)phenyl]borate) gives the dinuclear chloro-bridged nickel complex [Ni2(μ-Cl)2(dtbpe)2](BArF4)2 (1). [Ni(solv)6](BF4)2 reacts with dtbpe to give, depending on the solvent, the fluoro-bridged complex [Ni2(μ-F)2(dtbpe)2](BF4)2 (2) (solv = THF) or the mononuclear chelate complex [Ni(MeCN)2(dtbpe)](BF4)2 (3) (solv = MeCN). In 1-3, nickel cations are coordinated in a square-planar fashion according to X-ray crystallography. No Ni-Ni interaction was observed in dinuclear halogen-bridged complexes 1 and 2. 相似文献
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Dönnecke D Schweinitz A Stürzebecher A Steinmetzer P Schuster M Stürzebecher U Nicklisch S Stürzebecher J Steinmetzer T 《Bioorganic & medicinal chemistry letters》2007,17(12):3322-3329
Highly potent and selective substrate analogue factor Xa inhibitors were obtained by incorporation of non-basic or modestly basic P1 residues known from the development of thrombin inhibitors. The modification of the P2 and P3 amino acids strongly influenced the selectivity and provided potent dual factor Xa and thrombin inhibitors without affecting the fibrinolytic enzymes. Several inhibitors demonstrated excellent anticoagulant efficacy in standard clotting assays in human plasma. 相似文献