排序方式: 共有4条查询结果,搜索用时 172 毫秒
1
1.
Qing Ji a Jufei Lu b Qing Chao a Yan Zhang a Meijing Zhang a Minghong Gu b Mingliang Xu a c a National Maize Improvement Center China Agricultural University Beijing China b Key lab for plant biotechnology of Jiangsu province Yangzhou University Yangzhou China c Key Laboratory of Crop Genetic Improvement China 《Acta Genetica Sinica》2010,(1)
The rice indica/japonica hybrid shows strong heterosis.However,such inter-subspecific hybrid can't be directly used in rice production due to its low spikelet fertility.The S5 locus was proved to be associated with fertility of indica/japonica hybrid and its S5n allele from wide-compatibility variety (WCV) is capable to overcome fertility barrier.In the present study,we reported the causal sites in the S5 locus responsible for compatibility of indica/japonica hybrid.Fine-mapping of the S5 locus using the 11... 相似文献
2.
小鼠Nanog基因的克隆及其在大肠杆菌中的表达 总被引:4,自引:0,他引:4
按照nanog基因编码序列设计合成引物,利用RT-PCB从小鼠的囊胚期胚胎中扩增得到该 基因,并将该基因克隆到pET-28b(+)载体上,获得pET-28b(+)-nanog原核表达重组质粒,限制 性酶分析和DNA序列测定均证实该克隆插入片段为nanog基因编码序列。重组质粒转化大肠杆 菌BL21(DE3),经IPTG诱导表达,在大肠杆菌表达系统中获得了高效表达,western杂交证实该 蛋白具有6-His抗原活性,从而证实目的蛋白为Nanog蛋白。 相似文献
3.
Jie Lan Song Hua Hailin Zhang Yongli Song Jun Liu Yong Zhang Key Laboratory of Animal Reproductive Physiology & Embryo Technology Institution of biotechnology College of Veterinary Medicine Northwest A & F University Yangling China 《Acta Genetica Sinica》2010,(5)
In order to investigate DNA methylation profiles of five pluripotency-related genes(Oct4,Sox2,Nanog,Rex1 and Fgf4)during bovine maternal to zygotic transition(MZT)in both in vitro fertilized(IVF)and nuclear transfer(NT)embryos,sodium bisulfite sequencing method was used to detect DNA methylation levels,accompanied by the statistical analysis of embryo developmental rates.The results showed that Oct4,Nanog,Rex1 and Fgf4 were respectively demethylated by 25.22%(P < 0.01),3.84%(P > 0.05),31.82%(P < 0.01)and 10... 相似文献
4.
1