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1.
A D Ado R M Zolotareva B M Zolotarev 《Biulleten' eksperimental'no? biologii i meditsiny》1986,102(9):377-379
Method has been modified and used for quantitative gas chromatographic determination of microtraces in a large volume of test mixture under study. The method can be applied for the differentiated determination of genuine and false blood cholinesterase by final products of specific substrate hydrolysis. 相似文献
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Structures of the rye chloroplast DNA regions, containing psbD and psbI genes coding for the components of the reaction centre of photosystem II, D2 protein and I polypeptide, respectively, have been determined. The gene trnS for tRNA(Ser) (GCU) is located 111 bp downstream from the stop codon of the psbI gene on the opposite strand. The high homology between the rye BamHI-fragment comprising these genes and his counterpart from wheat are discussed. 相似文献
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EcoRI and BamHI fragments of rye chloroplast DNA comprising psbA gene were cloned and a 2729 bp region was sequenced. Cloning of EcoRI fragment into pTZ19R plasmid led to a single nucleotide deletion in the coding region of psbA gene. A scheme of full-length psbA gene cloning is proposed, allowing one to escape the damage effect of the psbA gene expression product on the host cell. The differences between monocot and dicot in nucleotide sequences of DNA downstream of psbA genes are discussed. Gene rps19 is located 131 bp downstream from psbA gene on the complementary strand. The amino acid sequences of D1 and S19 proteins of different species are compared. 相似文献
5.
Brodsky V. Y. Zolotarev Y. A. Malchenko L. A. Andreeva L. A. Lazarev D. S. Butorina N. N. Kozik V. S. Myasoedov N. F. 《Russian Journal of Developmental Biology》2020,51(2):99-105
Russian Journal of Developmental Biology - To clarify the organizing effect of Semax and HLDF-6 peptides on the kinetics of protein synthesis in hepatocytes, in addition to an in vitro study... 相似文献
6.
Zolotarev Yu. A. Dadayan A. K. Kozik V. S. Nagaev I. Yu. Azev V. N. Gorbunova E. Yu. Mustaeva L. G. Bogachouk A. P. Lipkin V. M. Myasoedov N. F. 《Russian Journal of Bioorganic Chemistry》2020,46(6):1044-1051
Russian Journal of Bioorganic Chemistry - The HLDF-6 hexapeptide corresponded to the 41–46 (TGENHR) fragment of the Human Leukemia Differentiation Factor (HLDF) and exhibited a wide spectrum... 相似文献
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Zolotarev Yu. A. Dadayan A. K. Kozik V. S. Shram S. I. Azev V. N. Bogachouk A. P. Lipkin V. M. Myasoedov N. F. 《Russian Journal of Bioorganic Chemistry》2019,45(6):514-521
Russian Journal of Bioorganic Chemistry - Pharmacokinetics of the promising antitumor peptide HLDF-6-AA (Ac-ThrGlyGluAsnHisArg-NH2) was studied using its uniformly tritiated derivative. Experiments... 相似文献
8.
Dolotov OV Zolotarev IuA Dorokhova EM Andreeva LA Alfeeva LIu Grivennikov IA Miasoedov NF 《Bioorganicheskaia khimiia》2004,30(3):241-246
The binding characteristics of the peptide Semax (Met-Glu-His-Phe-Pro-Gly-Pro) to plasma membranes of basal nuclei of the rat forebrain and the dynamics of its degradation during its incubation with these membranes were studied. Binding of the homogeneously labeled [G-3H]Semax was shown to be time-dependent, specific, and reversible. Specific binding of the heptapeptide depended on calcium ions and was characterized by the dissociation constant of the ligand-receptor complex Kd = 2.41 +/- 1.02 x 10(-9) M and by the concentration of binding sites Bmax = 33.5 +/- 7.9 x 10(-15) mol/mg of protein. A method of studying Semax biodegradation in the presence of plasma membranes of rat brain was developed. It is based on the use of the peptide homogeneously labeled with tritium and on an HPLC analysis with UV detection at 220 and 254 nm of the peptide fragments formed. The half-life of Semax in the presence of the plasma membranes was demonstrated to be longer than 1 h. Dipeptidylaminopeptidases are considered to be the main enzymes responsible for its biodegradation; they successively cleave Semax to the HFPGP pentapeptide and the PGP tripeptide. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 3; see also http://www.maik.ru. 相似文献
9.
Characteristics of annual production based on various mass data (whole animal mass; shell mass; and wet, dry, and ash-free dry soft tissue mass), the P/B coefficient, and specific production were estimated for the bivalve mollusk Chamelea gallinasampled from 21 localities in the northwestern and eastern Black Sea. The annual production correlated with population biomass (r= 0.994) and mean individual mass (r= –0.737), while the P/Bcoefficient depended mainly on mean individual mass (r= –0.719). Estimates of the P/Bcoefficient in Ch. gallinafrom the Black Sea were smaller than those of other marine invertebrates found worldwide with the same energy equivalent. Multiple linear regression equations were calculated for empirical relationships of various characteristics of annual production to the population biomass and mean individual biomass of Ch. gallina. 相似文献
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