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Sukhanova E. V. Shtykova Yu. R. Suslova M. Yu. Pestunova O. S. Kostornova T. Ya. Khanaev I. V. Zimens E. A. Podlesnaya G. V. Parfenova V. V. 《Microbiology》2019,88(3):324-334
Microbiology - A total of 170 heterotrophic bacterial strains were isolated from Lake Baikal epilithic biofilms. Identification of the isolates was carried out using the 16S rRNA gene sequencing... 相似文献
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Galachyants A. D. Tomberg I. V. Shtykova Yu. R. Sukhanova E. V. Suslova M. Yu. Zimens E. A. Blinov V. V. Sakirko M. V. Domysheva V. M. Belykh O. I. 《Biology Bulletin》2019,46(10):1377-1381
Biology Bulletin - Abstract—The aquatic surface microlayer at the interface between the atmosphere and the hydrosphere occupies 70% of the Earth’s surface, covering all water bodies.... 相似文献
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E. V. Sukhanova E. A. Zimens V. V. Parfenova O. I. Belykh 《Moscow University Biological Sciences Bulletin》2017,72(4):211-217
Many bacterial secondary metabolites, including pharmacologically promising compounds, are synthesized by polyketide synthases (PKS) enzyme complexes. In the present work, nucleotide sequences of the genes encoding 16S rRNA and PKS of heterotrophic bacterial strains isolated from epilithic biofilms of the littoral zone of Lake Baikal were determined. On the basis of molecular phylogenetic analysis of the 16S rRNA genes, six heterotrophic strains were identified: Serratia fonticola 1А and 10А, Pseudomonas umsongensis K10-2 and K10-3, Rheinheimera tilapiae K18, and Flavobacterium sp. 43-09. Sequencing of cloned amplification products for PKS gene cluster revealed 33 sequences. Genes involved in biosynthesis of antibiotics (difficidine, erythromycin, curacin, mixalamide, corallopyronin, and myxothiazol) and cytostatics (romidepsin, spiruchostatin, and disorazol) were found among homologous sequences. The low homology (50–83%) of the PKS amino acid sequences of Baikal bacteria with sequences from GenBank attests to the potential capability of strains to produce new, not yet studied bioactive compounds. The obtained results show that the studied strains may be of practical interest for biotechnological application. 相似文献
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