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1.
Ł. Chrzanowski E. Kaczorek A. Olszanowski 《World journal of microbiology & biotechnology》2005,21(6-7):1273-1277
Summary The growth of Candida maltosa on hydrocarbons (dodecane and hexadecane) was influenced by adding various natural and synthetic surfactants. Microbial adhesion
to the hydrocarbon was used to measure the surface cell hydrophobicity of the yeast, which in the presence of a synthetic
surfactant correlated with the degree of hydrocarbon biodegradation. Non-ionic surfactants caused the highest degree of hydrocarbon
biodegradation corresponding the lowest hydrophobicity. A different correlation was observed with natural surfactants, of
which saponin was the most effective for hydrocarbon biodegradation, though the concentration of this surfactant had no influence
on surface cell hydrophobicity. 相似文献
2.
Pseudomonas stutzeri, isolated from crude oil-contaminated soil, was used to degrade diesel oil. Of three surfactants, 120?mg rhamnolipids 1(-1) significantly increased degradation of diesel oil giving 88% loss after 14?days compared to 54% loss without the surfactant. The system with rhamnolipids was characterised by relatively high particle homogeneity. However, the addition of saponins to diesel oil caused the cells to aggregate (the polydispersity index: 0.542) and the biodegradation of diesel oil was only 46%. The cell yield was 0.22?g?l(-1). 相似文献
3.
Ewa Kaczorek Karolina Cieślak Katarzyna Bielicka-Daszkiewicz Andrzej Olszanowski 《Indian journal of microbiology》2013,53(1):84-91
Twelve different bacteria–yeast combinations were tested for determination of their ability to biodegrade diesel oil. The cell surface properties of the bacterial and yeast strains were correlated with the type of carbon source used in the experiments. The highest biodegradation of diesel oil after 7 days was obtained for the following combinations: Aeromonas hydrophila MR4–Yarrowia lipolytica EH 56 (87 %) and Xantomonas maltophila MRP7–Candida maltosa EH15 (90 %). Degradation performances of 10 of 12 combinations were enhanced by the presence of rhamnolipids. The highest increases were observed for A. hydrophila MR4–C. maltosa EH15 (from 34 to 67 %), A. hydrophila MR4–C. maltosa EH60 (from 47 to 76 %) and for Pseudomonas stutzeri MR7–C. maltosa EH60 (from 29 to 79 %). The addition of rhamnolipids to the system reduces the removal time of diesel oil from the contaminated water and changes the microbial adhesion to hydrocarbons. Modification of the cell surface of the tested strain during biodegradation is a very important factor determining the removal of hydrophobic compounds. 相似文献
4.
Ławniczak Łukasz Kaczorek Ewa Olszanowski Andrzej 《World journal of microbiology & biotechnology》2011,27(5):1183-1188
Recent biodegradation researches are focused on improving the deterioration efficiency and maintaining the stability of microbial
activity during the process. Immobilizing bacterial cells by using the adhesive method is a simple way to achieve both objectives.
The purpose of this study was to evaluate how the immobilization of bacterial cells by the biofilm forming method influences
the biodegradation efficiency of two different bacterial consortia—low diesel oil degraders (base deterioration efficiency < 40%)
and high phenol degraders (base deterioration efficiency > 90%). The degradation tests were carried out on four different
carriers (expanded clay pellets, polypropylene, polyvinyl chloride rings and paperboard). The obtained results show that biofilms
may considerably increase the efficiency of low degraders. The best diesel oil deterioration (80%) was achieved when paperboard
was used as a carrier. However, the immobilization of high degraders did not significantly influence their base biodegradation
potential. A 6% increase was noted only for the expanded clay pellets. 相似文献
5.
Ziemowit Zietkowski Roman Skiepko Maria M Tomasiak Anna Bodzenta-Lukaszyk 《Respiratory research》2007,8(1):76-9
Background
Exercise-induced bronchoconstriction (EIB) is a highly prevalent condition, whose pathophysiology is not well understood. Endothelins are proinflammatory, profibrotic, broncho- and vasoconstrictive peptides which play an important role in the development of airway inflammation and remodeling in asthma. The aim of the study was to evaluate the changes in endothelin-1 levels in exhaled breath condensate following intensive exercise in asthmatic patients.Methods
The study was conducted in a group of 19 asthmatic patients (11 with EIB, 8 without EIB) and 7 healthy volunteers. Changes induced by intensive exercise in the concentrations of endothelin-1 (ET-1) in exhaled breath condensate (EBC) during 24 hours after an exercise challenge test were determined. Moreover, the possible correlations of these measurements with the results of other tests commonly associated with asthma and with the changes of airway inflammation after exercise were observed.Results
In asthmatic patients with EIB a statistically significant increase in the concentration of ET-1 in EBC collected between 10 minutes and 6 hours after an exercise test was observed. The concentration of ET-1 had returned to its initial level 24 hours after exercise. No effects of the exercise test on changes in the concentrations of ET-1 in EBC in either asthmatic patients without EIB or healthy volunteers were observed. A statistically significant correlation between the maximum increase in ET-1 concentrations in EBC after exercise and either baseline FENO and the increase in FENO or BHR to histamine 24 hours after exercise in the groups of asthmatics with EIB was revealed.Conclusion
The release of ET-1 from bronchial epithelium through the influence of many inflammatory cells essential in asthma and interactions with other cytokines, may play an important role in increase of airway inflammation which was observed after postexercise bronchoconstriction in asthmatic patients. 相似文献6.
Ziemowit Zietkowski Maria M Tomasiak-Lozowska Roman Skiepko Elzbieta Zietkowska Anna Bodzenta-Lukaszyk 《Respiratory research》2010,11(1):110
Background
Airway eosinophilia is considered a central event in the pathogenesis of asthma. Eotaxin plays a key role in selective eosinophil accumulation in the airways and, subsequently, their activation and degranulation. The study was undertaken to evaluate eotaxin-1 levels in the exhaled breath condensate (EBC) of asthmatics with different degrees of asthma severity and to establish the possible correlation of these measurements with other recognized parameters of airway inflammation.Methods
EBC was collected from 46 patients with allergic asthma (14 with steroid-naïve asthma, 16 with ICS-treated, stable asthma, 16 with ICS-treated unstable asthma) and 12 healthy volunteers. Concentrations of eotaxin-1 were measured by ELISA.Results
In the three groups of asthmatics, eotaxin-1 concentrations in EBC were significantly higher compared with healthy volunteers (steroid-naïve asthma: 9.70 pg/ml ± 1.70, stable ICS-treated asthma: 10.45 ± 2.00, unstable ICS-treated asthma: 17.97 ± 3.60, healthy volunteers: 6.24 ± 0.70). Eotaxin-1 levels were significantly higher in patients with unstable asthma than in the two groups with stable disease. We observed statistically significant correlations between the concentrations of eotaxin-1 in EBC and exhaled nitric oxide (FENO) or serum eosinophil cationic protein (ECP) in the three studied groups of asthmatics. We also discovered a significantly positive correlation between eotaxin-1 in EBC and blood eosinophil count in the groups of patients with unstable asthma and steroid-naïve asthma.Conclusions
Measurements of eotaxin-1 in the EBC of asthma patients may provide another useful diagnostic tool for detecting and monitoring airway inflammation and disease severity. 相似文献7.
8.
A. Pijanowska E. Kaczorek Ł. Chrzanowski A. Olszanowski 《World journal of microbiology & biotechnology》2007,23(5):677-682
Biodegradation and hydrophobicity of Pseudomonas spp. and Bacillus spp. strains were tested at different concentrations of the biosurfactant Quillaya saponin. A model mixture of hydrocarbon
(dodecane and hexadecane) was used for estimating the influence of surfactants on biodegradation. The bacterial adhesion to
hydrocarbon method for determination of bacterial cell surface hydrophobicity was exploited. Among the tested bacterial strains
the higher hydrophobicity was noticed for Pseudomonas aeruginosa TK. The hydrophobicity of this strain was 84%. The highest hydrocarbon biodegradation was observed for P. aeruginosa TK (49%) and Bacillus subtilis (35%) strains after 7 days of experiments. Generally the addition of Quillaya saponin increased hydrocarbon biodegradation
remarkably. The optimal concentration proved to be 80 mg l−1. The degree of hydrocarbon biodegradation was 75% for P. aeruginosa TK after the addition of saponin. However the most significant increase in biodegradation after addition of Quillaya saponin
was in the case of P. aeruginosa 25 and Pseudomonas putida (the increase of biodegradation from 21 to 52% and from 31 to 66%, respectively). It is worth mentioning that decrease of
hydrophobicity is correlated with the best biodegradation by P. aeruginosa strain. For the remaining strains, no significant hydrophobicity changes in relation to the system without surfactant were
noticed. 相似文献
9.
Yeast and bacteria cell hydrophobicity and hydrocarbon biodegradation in the presence of natural surfactants: rhamnolipides and saponins 总被引:1,自引:0,他引:1
This study concerns the relation between hydrocarbon biodegradation in the presence of natural surfactants and cell hydrophobicity resulting from the use of these surfactants. The relative capabilities of two bacterial strains (Pseudomonas aeruginosa and Bacillus subtilis) and two yeast strains (Candida maltosa, Yarrowia lipolytica) were investigated. The selected microorganisms were tested separately and in combination in order to achieve the optimal degrading performance. The surface cell hydrophobicity of microorganisms and the degree of hydrocarbon biodegradation were measured. The microbial adhesion to the hydrocarbon (MATH) test was used to denote the surface cell hydrophobicity of the microbial species. The results indicate the correlation between the modification of the surface cell and the degree of hydrocarbon biodegradation; however results for bacteria differ from that obtained for yeast strains. Saponins, as the surfactant, was more effective than rhamnolipides during hydrocarbon biodegradation, though the concentration of this surfactant has no significant influence on the surface cell hydrophobicity. 相似文献
10.
The main goals of this study were to investigate the relationships between densities of the breeding population of the middle spotted woodpecker Dendrocopos medius obtained on 19 large-scale plots, and the share of different habitats and age of forest stands. An average crude density based on an area defined arbitrarily by observers was 0.66±0.43 territories/10 ha, while the ecological density based on an area including habitats potentially used by organisms reached 1.09±0.64 territories/10 ha. Stepwise multiple regression indicated that the crude density was negatively affected by the share of coniferous forest thereby explaining 50% of variation. The ecological density was negatively correlated with the percentage of young deciduous stands (0–40 years old). This variable explained 36% of the total variation. These results suggest that on the level of macrohabitat analysis, the share of unsuitable forest stands seem to play a major role in determining the density of the middle spotted woodpecker. 相似文献