Frankia菌种保藏

对用4种方法保藏的Frankia菌,进行了培养物存活、形态及其固氮活性的检测.发现在无氮液体培养基中保藏6年的Frankia.菌丝断裂,孢囊不完整.同期经有氮液体保藏的Frankia菌孢囊较完整.冷冻干燥保藏3.5年和砂管保藏8年,孢囊和菌丝均较完整.上述方法保藏的菌种,经活化后均能生长,且具有典型的Frankia菌形态特征和固氮活性.4种方法比较,无氮液体保藏法的菌体细胞生长速度快,固氮活性强,有侵染结瘤能力.     

Novel quinazoline-quinoline alkaloids with cytotoxic and DNA topoisomerase II inhibitory activities

Two new synthetic analogues of luotonins A and F, 7-acetylaminoluotonin A (6) and 3-[3H(quinazolino-4-one)]quinoline (7) were synthesized. The new analogues, along with four natural quinazoline-quinoline alkaloids, luotonins A (1), B (2), E (3), F (4) and a synthetic deoxoluotonin F (5), showed cytotoxic activity (IC(50) 1.8-40.0 microg/mL) and DNA topoisomerase II inhibition at a concentration of 25 microM.     

芡实分区刈割试验对陈瑶湖后生浮游动物群落结构的影响

陈瑶湖流域是长江中下游重要的水禽省级自然保护区,近年来随着农业的发展,湖区存在芡实种植过密,汛期影响行洪的隐患,对湖区的水生态系统结构与功能的稳定性造成严重影响。为研究芡实分区刈割试验对陈瑶湖后生浮游动物群落结构的影响,于2019年8月期间对陈瑶湖后生浮游动物进行调查。共鉴定出后生浮游动物15科22属40种,其中轮虫12属26种,枝角类8属11种,桡足类2属3种,群落结构主要以轮虫为主,后生浮游动物种类分布时空上无显著差异(P>0.05)。共发现优势种8属10种,分别为萼花臂尾轮虫(Brachionus calyciflorus)、角突臂尾轮虫(Brachionus angularis)、剪形臂尾轮虫(Brachionus forficula)、螺形龟甲轮虫(Keratella cochlearis)、囊形单趾轮虫(Monostyla bulla)、蹄形腔轮虫(Lecane ungulata)、针簇多肢轮虫(Polyarthra trigla)、迈氏三肢轮虫(Filinia maior)、长额象鼻溞(Bosmina longirostris)和广布中剑水蚤(Mesocyclops...     

多环芳烃降解菌筛选及其降解特性

通过选择性富集培养,从辽河油田稠油污染土壤4号土样中,获得了能以高浓度菲(2000mg·L^-1)为唯一碳源和能源快速生长的优势菌系和优良菌株ZL5．16S rDNA核苷酸序列分析表明,ZL5菌株归类于鞘氨醇单胞菌属,分得的菌系和菌株有较强的降解菲能力,120h混合菌系降解了投加菲的95．28％,菌株降解了69．24％,但它们对芘的降解能力均较低,外加碳源葡萄糖可提高菌系和菌株的菲、芘降解能力,加量多。提高幅度大,但超过一定量。降解速率开始下降,表现出抑制效应。所以,应用时需控制适宜的浓度。     

降解芳烃微生物的多样性

芳烃是一类生物异源物质,自然微生物群落利用其对环境的适应性,对这类物质由陌生到适应,微生物群落的遗传背景发生了变化,降解芳烃的微生物呈现出多样性,本文系统介绍了降解芳烃微生物的特性,物种资源,环境适应,遗传背景及演变;介绍了各遗传型物种的功能基因数量,表达及调控方式,指明芳烃环境污染的生物修复主要取决于高效工程构造及代谢过程的控制。     

苦草属植物雄花果实种子显微结构的研究

苦草属植物体外部形态相似,叶线行或带形,野外难以直接鉴定到种,而雄花雄蕊数目、果实和种子的显微结构是苦草属(Vallisneria)植物物种的鉴别性特征之一。苦草属物种的雄花小,仅有1.5 mm,人工解剖雄花观察方法难以实现雄花雄蕊数目的识别;本研究采用雄株雄佛焰苞水培预培养技术获取自然开放的雄花并利用解剖学显微镜对3种苦草属植物雄花、果实和种子进行了解剖观察和研究。显微结构观察表明,苦草(V. natans)雄蕊1枚,果实圆柱形,表面光滑,种子无翅,具纵条纹;刺苦草(V. spinulosa)雄蕊2枚,果实三棱形,棱上具刺,种子具翅;密刺苦草(V. denseserrulata)雄蕊2枚,果实三棱圆柱形,表面光滑,种子无翅。依据文献,密刺苦草、刺苦草、长梗苦草(V. longipedunculata)和安徽苦草(V. anhuiensis)是安徽苦草属新分布记录种,其中长梗苦草和安徽苦草是分布于安徽的新种。     

银叶老鹳草种群中诱导保护抗性的研究

银叶老鹳草种群中诱导保护抗性的研究刘登义,查德民,周忠泽劳斯·埃里克松（安徽师范大学生物系,芜湖２４１０００）（瑞典国立宇米欧大学生态植物学系）ＩｎｄｕｃｅｄＰｒｏｔｅｃｔｉｖｅＲｅｓｉｓｔａｎｃｅｉｎＧｒｅａｎｉｕｍｓｙｌｖａｔｉｃｕｍＰｏｐｕｌａ...     

Fine mapping and candidate gene analysis of the floury endosperm gene, FLO(a), in rice

In addition to its role as an energy source for plants, animals and humans, starch is also an environmentally friendly alternative to fossil fuels. In rice, the eating and cooking quality of the grain is determined by its starch properties. The floury endosperm of rice has been explored as an agronomical trait in breeding and genetics studies. In the present study, we characterized a floury endosperm mutant, flo(a), derived from treatment of Oryza sativa ssp. japonica cultivar Hwacheong with MNU. The innermost endosperm of the flo(a) mutant exhibited floury characteristics while the outer layer of the endosperm appeared normal. Starch granules in the flo(a) mutant formed a loosely-packed crystalline structure and X-ray diffraction revealed that the overall crystallinity of the starch was decreased compared to wild-type. The FLO(a) gene was isolated via a map-based cloning approach and predicted to encode the tetratricopeptide repeat domaincontaining protein, OsTPR. Three mutant alleles contain a nucleotide substitution that generated one stop codon or one splice site, respectively, which presumably disrupts the interaction of the functionally conserved TPR motifs. Taken together, our map-based cloning approach pinpointed an OsTPR as a strong candidate of FLO(a), and the proteins that contain TPR motifs might play a significant role in rice starch biosynthetic pathways.     

Profile of acute infectious markers in sporadic hepatitis E

Laboratory diagnosis of acute infection of hepatitis E virus (HEV) is commonly based on the detection of HEV RNA, IgM and/or rising IgG levels. However, the profile of these markers when the patients present have not been well determined. To clarify the extent of misdiagnosed sporadic hepatitis E in the initial laboratory detection, serial sera of 271 sporadic acute hepatitis cases were collected, detected and the dynamics of each acute marker during the illness course were analyzed. 91 confirmed cases of hepatitis E were identified based on the presentation of HEV RNA, IgM or at least 4 fold rising of IgG levels. 21 (23.1%) hepatitis E cases were false negative for the viral RNA and 40 (44.0%) for rising IgG, because occurrence of these markers were confined to acute phase of infection and viremia had already subsided and antibody level peaked when these patients presented. IgM was detected in 82 (90.1%) cases. It is the most prevalent of the three markers, because the antibody persisted until early convalescence. Nine cases negative for IgM were positive for rising IgG and one was also positive for the viral RNA; all of these nine cases showed high avid IgG in their acute phase sera, which indicated re-infection. In summary, it is not practicable to determine the true occurrence of sporadic hepatitis E. Nevertheless, it could be closely approximated by approach using a combination of all three acute markers.     

Proteomic and phosphoproteomic landscape of salivary extracellular vesicles to assess OSCC therapeutical outcomes

Circulating extracellular vesicles (EVs) have emerged as an appealing source for surrogates to evaluate the disease status. Herein, we present a novel proteomic strategy to identify proteins and phosphoproteins from salivary EVs to distinguish oral squamous cell carcinoma (OSCC) patients from healthy individuals and explore the feasibility to evaluate therapeutical outcomes. Bi-functionalized magnetic beads (BiMBs) with Ti (IV) ions and a lipid analog, 1,2-Distearoyl-3-sn-glycerophosphoethanolamine (DSPE) are developed to efficiently isolate EVs from small volume of saliva. In the discovery stage, label-free proteomics and phosphoproteomics quantification showed 315 upregulated proteins and 132 upregulated phosphoproteins in OSCC patients among more than 2500 EV proteins and 1000 EV phosphoproteins, respectively. We further applied targeted proteomics by coupling parallel reaction monitoring with parallel accumulation-serial fragmentation (prm-PASEF) to measure panels of proteins and phosphoproteins from salivary EVs collected before and after surgical resection. A panel of three total proteins and three phosphoproteins, most of which have previously been associated with OSCC and other cancer types, show sensitive response to the therapy in individual patients. Our study presents a novel strategy to the discovery of effective biomarkers for non-invasive assessment of OSCC surgical outcomes with small amount of saliva.