The Mechanisms of Stimulation of Migration and Invasion of Tumor Cells by Extracellular Heat Shock Protein 90 (eHsp90) in vitro

Biophysics - Extracellular heat shock protein 90 (eHsp90) plays an important role in cell motility, invasion, and metastasis of tumor cells. eHsp90 stimulates migration and invasion of cells via...     

Internalization by Cells and Antitumor Activity of Antibodies and Immunotoxins Specific for the Heat Shock Protein 90 β Isoform

Mouse monoclonal antibodies to Hsp90β (β isoform of heat shock protein 90) have been shown to bind specifically to Hsp90β localized on the surface of tumor and nontransformed cells. After binding to the membrane-associated Hsp90β, the antibodies actively dissociated into the culture medium and were also internalized by the cells. An immunoconjugate based on the Hsp90β-specific antibody and the cytotoxic agent mertansine did not have high cytotoxic activity for tumor cells in vitro. Administration of Hsp90β-specific antibodies in mice did not affect the growth of the primary Lewis lung carcinoma, while tumor metastasis to the lungs decreased and the average lifespan of mice increased. The results indicate a certain therapeutic potential of antibodies to Hsp90β for the treatment of tumor diseases.

     

Cell-Impermeable Peptide Fragments of Survivin and Hsp70/Hsp90-Organizing Protein Inhibit the Hsp90-Dependent Migration and Invasion of Tumor Cells

Biophysics - Membrane-associated and secreted extracellular heat shock proteins 90 (Hsp90) have important roles in the migration, invasion, and metastasis of tumor cells and are proposed as...