广东滩涂经济鱼类寄生吸虫Ⅵ.独睾科二新种记述(复殖目)

本文对广东滩涂经济鱼类寄生复殖吸虫独睾科ＭｏｎｏｒｃｈｉｉｄａｅＯｄｈｎｅｒ,１９１１中的两新种分别进行详细的描述,其中细长椭囊吸虫Ｅｌｌｉｐｔｏｂｕｒｓａａｔｔｅｎｕａｔｕｓｓｐ．ｎｏｖ作为拟原穴亚科ＰｓｅｕｄｏｐｒｏｃｔｏｔｒｅｍａｔｉｎａｅＥｌｌｉｐｔｏｂｕｒｓａ属的新种,巨穴孤睾吸虫Ｌａｓｉｏｔｏｃｕｓｍａｒｏｔｒｅｍａｓｐ．ｎｏｖ作为孤睾亚科ＬａｓｉｏｔｏｃｉｎａｅＬａｓｉｏｔｏｃｕｓ属     

大鼠卵巢不同功能阶段时c-fos表达的动态变化

目的和方法：本文用免疫组织化学方法检测了成年大鼠卵巢于不同的功能阶段和未成年大鼠外源性激素诱导的卵巢于不同的功能阶段c-fos表达的变化以及与血清E2和P含量的相关关系。结果：在成年大鼠动情前期、动情期卵巢的间质腺和基质中及动情间期和妊娠期卵巢的黄体细胞和基质中有c-fos表达,c-fos蛋白阳性信号的表达范围和表达强度在动情前期卵巢较大,动情间期和动情期卵巢较低,妊娠期卵巢最大,这种变化与血清E2和P含量的变化呈明显的正相关关系。未成年大鼠,用DES使卵泡发育至窦前期时,在卵巢中未检测到有c-fos蛋白的表达,用PMSG使贸泡发育至排卵前期时在卵巢的间质腺和基质中有c-fos表达,用PMSG和hCG后4天形成的早期黄体化卵巢c-fos在黄体细胞和基质中的表达明显升高,而于用hCG后9天形成的晚期黄体化卵巢c-fos表达明显下降,这种变化也与血清E2和P含量的变化呈明显的正相关关系。结论：c-fos与卵泡发育、排卵、黄体形成和退化等功能密切相关。     

Hydrogen sulfide plays a potential alternative for the treatment of metabolic disorders of diabetic cardiomyopathy

Molecular and Cellular Biochemistry - Diabetic cardiomyopathy (DCM) is a cardiovascular complication that tends to occur in patients with diabetes, obesity, or insulin resistance, with a higher...     

表面残留杀虫剂对蜘蛛的影响研究

以前的报道多集中于研究杀虫剂直接喷洒对蜘蛛的影响。本研究以三突花蛛和沟渠豹蛛为对象,测定了溴氰菊酯、毒死蜱和查虫清（植物源杀虫剂）共3种杀虫剂的表面残留对蜘蛛的驱避作用和急性毒性。结果表明：残留2h的毒死蜱和查虫清对三突花蛛无驱避作用,而残留2h的溴氰菊酯对三突花蛛有明显地驱避作用。3种杀虫剂的2h表面残留对沟渠豹蛛均有显著的驱避作用,其中溴氰菊酯的驱避作用最强。残留24h的3种杀虫剂对三突花蛛和沟渠豹蛛无明显的驱避作用。表面残留的毒死蜱和查虫清对蜘蛛的毒性小,溴氰菊酯的残留对三突花蛛和沟渠豹蛛毒性大。表面残留2h的溴氰菊酯对沟渠豹蛛的毒性显著高于残留24h的溴氰菊酯。     

Cytological mechanism of pollen abortion resulting from allelic interaction of F1 pollen sterility locus in rice (Oryza sativa L.)

Pollen abortion is one of the major reasons causing the inter-subspecific F₁ hybrid sterility in rice and is due to allelic interaction of F₁ pollen sterility genes. The microsporogenesis and microgametogenesis of Taichung 65 and its three F₁ hybrids were comparatively studied by using techniques of differential interference contrast microscopy, semi-thin section light microscopy, epifluorescence microscopy and TEM. The results showed that there were differences among the cytological mechanisms of pollen abortion due to allelic interaction at the three F₁ pollen sterility loci. The allelic interaction at S-a locus resulted in microspores unable to extend the protoplasm membrane with the enlargement of the microspore at the middle microspore stage and finally producing empty abortive pollen. The allelic interaction at S-b locus caused asynchronous development of microspores at the middle microspore stage producing stainable abortive pollen. The allelic interaction at S-c locus mainly led to the non-dissolution of the generative cell wall and finally caused the hybrid F₁ mainly producing stainable abortive pollen. Genotypic identification indicated that the abortive pollen were those with S ^j allele.     

PCSK9 siRNA inhibits HUVEC apoptosis induced by ox-LDL via Bcl/Bax–caspase9–caspase3 pathway

This paper investigated the effects of ox-LDL on PCSK9, and the molecular mechanisms of PCSK9 siRNA-inhibited apoptosis induced by ox-LDL in human umbilical vein endothelial cells (HUVECs), to clarify the role of PCSK9 in atherosclerogenesis. HUVECs were incubated with ox-LDL for 24?h. The apoptosis was observed by Hoechst 33258 staining. The expression of PCSK9, LOX-1 mRNAs and proteins was detected by RT-PCR, western blot, respectively. The PCSK9 siRNAs labeled with fluorescence were transfected into HUVECs by Lipofectamine 2000. After transfection for 24?h, cells were treated with ox-LDL for 24?h, HUVECs apoptosis transfected siRNA was detected by Hoechst 33258 staining and flow cytometer. The expression of Bcl-2, Bax, caspase3, 8, 9 was detected by western blot. The activity of caspase3, 9 was detected by kits. Our results showed that apoptosis of HUVECs and the expressions of PCSK9 and LOX-1 were upregulated secondary to induction by ox-LDL in a concentration-dependent manner. However, ox-LDL-induced HUVEC apoptosis and PCSK9 expression, but not LOX-1 expression, were significantly reduced by PCSK9 siRNA. These results demonstrate a linkage between HUVEC apoptosis and PCSK9 expression. Furthermore, we detected the possible pathway involved in apoptotic regulation by PCSK9 siRNA; our results showed that the expression of Bcl-2 decreased, whereas that of Bax increased. In addition, ox-LDL enhanced the activity of caspase9 and then caspase3. Pretreatment of HUVECs with PCSK9 siRNA blocked these effects of ox-LDL. These findings suggest that ox-LDL-induced HUVECs apoptosis could be inhibited by PCSK9 siRNA, in which Bcl/Bax-caspase9-caspase3 pathway maybe was involved through reducing the Bcl-2/Bax ratio and inhibited the activation of both caspase9 and 3.     

Identification of a new gene encoding 5-enolpyruvylshikimate-3-phosphate synthase using genomic library construction strategy

Applying the genomic library construction strategy and colony screening, a new aroA gene encoding 5-enolpyruvylshikimate-3-phosphate synthase has been identified, cloned and overexpressed in Escherichia coli, and the enzyme was purified to homogeneity. Kinetic analysis of the AroA _{P.fluorescens} indicated that the full-length enzyme exhibits 10-fold increased IC50 and an approximately 38-fold increased K _i for glyphosate compared to those of the AroA _E.coli , while retaining high affinity for the substrate phosphoenolpyruvate. Furthermore, we have transformed the new aroA _{P.fluorescens} gene into Arabidopsis thaliana via a floral dip method, and demonstrated that transgenic A. thaliana plants exhibit significant glyphosate resistance when compared with the wild type.     

石墨炉原子吸收光谱法测定虾产品中铝的方法研究

目的:本文着重研究利用石墨炉原子吸收光谱法测定虾产品中铝,对测定条件进行优化选择,达到快速、准确,以弥补国家标准检测方法中没有虾产品中铝检测方法的不足。方法:利用混合酸硝酸-高氯酸对样品进行消化处理,无基体改进剂,利用石墨炉原子吸收光谱仪进行定量检测。结果:在优化干燥、灰化、原子化、除残四个步骤的时间和温度的实验条件后,铝含量在0.0-50.0μg/L范围内呈线性关系,相关系数达到r=0.9990,最低检出限为0.54 ng/mL,铝的特征质量为5.0 pg。相对标准偏差为3.83%-6.42%,虾产品中铝含量测定回收率为90.7%-105.6%。结论:本法方便准确,灵敏度高,并有较高的回收率,可作为日常检测使用。