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An efficient process for the production of fuel ethanol from bamboo that consisted of hydrolysis with concentrated sulfuric acid, removal of color compounds, separation of acid and sugar, hydrolysis of oligosaccharides and subsequent continuous ethanol fermentation was developed. The highest sugar recovery efficiency was 81.6% when concentrated sulfuric acid hydrolysis was carried out under the optimum conditions. Continuous separation of acid from the saccharified liquid after removal of color compounds with activated carbon was conducted using an improved simulated moving bed (ISMB) system, and 98.4% of sugar and 90.5% of acid were recovered. After oligosaccharide hydrolysis and pH adjustment, the unsterilized saccharified liquid was subjected to continuous ethanol fermentation using Saccharomycescerevisiae strain KF-7. The ethanol concentration, the fermentation yield based on glucose and the ethanol productivity were approximately 27.2 g/l, 92.0% and 8.2 g/l/h, respectively. These results suggest that the process is effective for production of fuel ethanol from bamboo. 相似文献
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Xiao-He Wu Hai-Long Yu Zhao-Yong Ba Jing-Yu Chen Hong-Gang Sun Bei-Zhong Han Professor 《Biotechnology journal》2010,5(1):75-84
To select an appropriate sampling method for comparison of metabolite profiles between planktonic and biofilm Staphylococcus aureus using NMR techniques, we evaluated three methods: quenching-centrifugation (QC), filtration-quenching (FQ) and filtration-quenching-lyophilization (FQL). We found differences in metabolite loss, yield, reproducibility and metabolite profile. QC caused severe metabolite leakage and possible decomposition of nucleotides. FQ achieved high yields and reproducibility, although it had the disadvantages of long filtration and rinse times before quenching. FQL resulted in a loss of a few metabolites and a lower yield due to lyophilization. Although the biomarkers discovered by each method were nearly the same and seemed insensitive to technical variances, we conclude that FQ is the most appropriate sampling method because of its high yield and reproducibility. 相似文献
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土壤呼吸是陆地碳循环中仅次于全球总初级生产力的第二大碳通量途径, 揭示土壤呼吸的时空格局对整个陆地碳循环具有重要意义。该文在中国东部亚热带季风气候区, 按纬度梯度由南向北选取深圳梧桐山、杨东山十二度水保护区、宁波天童山3个区域作为研究对象, 于2009年8月至2010年10月测定了不同季节各个区域内代表性植被类型的土壤呼吸速率及地下5 cm处土壤温度, 旨在初步了解中国东部亚热带森林地区土壤呼吸的时空格局及其影响因素。结果显示: 3个区域的土壤呼吸速率均存在显著的季节变化, 其变幅为2.64-6.24 μmol CO2·m -2·s-1, 总体趋势和地下5 cm处土壤温度的季节变化一致, 均为夏季最高冬季最低; 土壤温度的变化可以解释不同样地土壤呼吸季节变化的58.3%-90.2%; 各样地全年的Q10值从1.56到3.27; 通过离样地最近的气象站点的日平均气温与试验样地地下5 cm处土壤温度之间的线性正相关关系推算出日土壤温度的变化, 利用土壤呼吸速率和地下5 cm处土壤温度之间的指数关系, 估算出各样地全年的土壤CO2通量为1 077-2 058 g C·m-2·a-1, 在全球所有生态系统类型中处于较高水平。 相似文献
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Li Yun-Cheng Zeng Wei-Yi Gou Min Sun Zhao-Yong Xia Zi-Yuan Tang Yue-Qin 《Applied microbiology and biotechnology》2017,101(20):7741-7753
Applied Microbiology and Biotechnology - It is of utmost importance to construct industrial xylose-fermenting Saccharomyces cerevisiae strains for lignocellulosic bioethanol production. In this... 相似文献
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An-Kai Zhu Sa-Sa Li Shu-Pei Yu Zhao-Yong Zhang Huan Li Jing-Feng Li Shan-Shan Gao Heng Chen Jincun Zhao Run Shi Mingjian Lu Chaoyang Li 《中国病毒学》2022,37(6):942-945
Highlights
1. Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, Western blotting, and immunofluorescence staining.
2. A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA.
3. The mAb, 2G11, shows 97.4% sensitivity and 100% specificity for diagnosing the human blood samples. 相似文献
1. Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, Western blotting, and immunofluorescence staining.
2. A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA.
3. The mAb, 2G11, shows 97.4% sensitivity and 100% specificity for diagnosing the human blood samples. 相似文献
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Zhang Y Hassan MQ Li ZY Stein JL Lian JB van Wijnen AJ Stein GS 《Journal of cellular biochemistry》2008,105(2):487-496