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V. A. Kulichkova Yu. Ya. Zaykova Yu. B. Ermolaeva E. V. Vinogradova L. Huang A. N. Tomilin N. A. Barlev A. S. Tsimokha 《Cell and Tissue Biology》2014,8(4):330-336
In the present work, a stable cell line based on human embryonic kidney HEK293 cells, which expresses the proteasome subunit PSMD14 fused to the fluorescent protein EGFP and HTBH tag, has been selected. This protein, PSMD14-EGFP-HTBH, was shown to be completely incorporated into proteasomes, and such complexes have a chymotrypsin-like peptidase activity that is fundamental for proteasomes. The constructed and selected cell line can be used for further fluorescent studies of localization of proteasomes in the cell. 相似文献
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Ilina E. N. Solopova O. N. Balabashin D. S. Larina M. V. Aliev T. K. Grebennikova T. V. Losich M. A. Zaykova O. N. Sveshnikov P. G. Dolgikh D. A. Kirpichnikov M. P. 《Russian Journal of Bioorganic Chemistry》2018,44(6):695-704
Russian Journal of Bioorganic Chemistry - Rabies is a zoonotic disease, for which effective treatment methods after the onset of clinical symptoms have not been developed yet. Polyclonal sera, both... 相似文献
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