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1.
Prey selection of the shallow water fishPomatoschistus minutus (Gobiidae,Teleostei) in the SW Baltic
C. Dieter Zander 《Helgoland Marine Research》1990,44(2):147-157
The aim of this investigation is to demonstrate the reasons of an opportunist feeder to select some components of the available
food supply and to avoid others. Object of this test is the eurytopic and euryhaline sand goby,Pomatoschistus minutus (Pallas), from the Luebeck Bight. It inhabits both sand habitats as well as bottoms mixed with pebbles or continuous hard
substrates. Therefore, it is often in close contact withMytilus-belts which present a very rich food supply of small crustaceans. The selectivity behaviour of sand gobies from a sand/clay
ecotone was compared with that of populations from a stony pier and a sand bottom during the reproduction period. Regarding
the numbers of prey organisms, crustaceans of the periphyton were generally preferred at the pier but avoided in the ecotone.
The same is valid for prey organisms of the psammal which were preferred by gobies of the sand bottom but mostly avoided by
populations of mixed bottoms. Analyses of size selections revealed that the preferred gammarids orJaera isopods were between 2 and 5 mm length. A balanced relation of goby biomass and utilizable food supply (predatory impact
index) seems to effect positive selectivity of gammarids in theMytilus-belts or of harpacticoids in the sand bottom. 相似文献
2.
Use of avidin-sepharose to isolate and idenify biotin polypeptides from crude extracts 总被引:4,自引:0,他引:4
A method of isolating and identifying biotin polypeptides from crude cellular extracts is described. Protein samples are run on small avidin-Sepharose columns. After washing away nonspecifically bound protein, the biotin enzymes are eluted using an SDS-urea solution. The inactive polypeptides are then electrophoresed on SDS-polyacrylamide gels. The biotin polypeptides in the gels are identified by using fluorescent avidin or by analyzing for radioactive biotin-labeled polypeptides. A sensitive method for assaying biotin using avidin-Sepharose is also described. 相似文献
3.
Emily M. Duncan Annette C. Broderick Wayne J. Fuller Tamara S. Galloway Matthew H. Godfrey Mark Hamann Colin J. Limpus Penelope K. Lindeque Andrew G. Mayes Lucy C. M. Omeyer David Santillo Robin T. E. Snape Brendan J. Godley 《Global Change Biology》2019,25(2):744-752
Despite concerns regarding the environmental impacts of microplastics, knowledge of the incidence and levels of synthetic particles in large marine vertebrates is lacking. Here, we utilize an optimized enzymatic digestion methodology, previously developed for zooplankton, to explore whether synthetic particles could be isolated from marine turtle ingesta. We report the presence of synthetic particles in every turtle subjected to investigation (n = 102) which included individuals from all seven species of marine turtle, sampled from three ocean basins (Atlantic [ATL]: n = 30, four species; Mediterranean (MED): n = 56, two species; Pacific (PAC): n = 16, five species). Most particles (n = 811) were fibres (ATL: 77.1% MED: 85.3% PAC: 64.8%) with blue and black being the dominant colours. In lesser quantities were fragments (ATL: 22.9%: MED: 14.7% PAC: 20.2%) and microbeads (4.8%; PAC only; to our knowledge the first isolation of microbeads from marine megavertebrates). Fourier transform infrared spectroscopy (FT‐IR) of a subsample of particles (n = 169) showed a range of synthetic materials such as elastomers (MED: 61.2%; PAC: 3.4%), thermoplastics (ATL: 36.8%: MED: 20.7% PAC: 27.7%) and synthetic regenerated cellulosic fibres (SRCF; ATL: 63.2%: MED: 5.8% PAC: 68.9%). Synthetic particles being isolated from species occupying different trophic levels suggest the possibility of multiple ingestion pathways. These include exposure from polluted seawater and sediments and/or additional trophic transfer from contaminated prey/forage items. We assess the likelihood that microplastic ingestion presents a significant conservation problem at current levels compared to other anthropogenic threats. 相似文献
4.
Esterhuizen Karien Lindeque J. Zander Mason Shayne van der Westhuizen Francois H. Rodenburg Richard J. de Laat Paul Smeitink Jan A. M. Janssen Mirian C. H. Louw Roan 《Metabolomics : Official journal of the Metabolomic Society》2021,17(1):1-11
Metabolomics - Studies investigating crop resistance to abiotic and biotic stress have largely focused on plant responses to singular forms of stress and individual biochemical pathways that only... 相似文献
5.
6.
Ana Casa?al Ulrich Zander Cristina Mu?oz Florine Dupeux Irene Luque Miguel Angel Botella Wilfried Schwab Victoriano Valpuesta José A. Marquez 《The Journal of biological chemistry》2013,288(49):35322-35332
Pathogenesis-related 10 (PR-10) proteins are involved in many aspects of plant biology but their molecular function is still unclear. They are related by sequence and structural homology to mammalian lipid transport and plant abscisic acid receptor proteins and are predicted to have cavities for ligand binding. Recently, three new members of the PR-10 family, the Fra a proteins, have been identified in strawberry, where they are required for the activity of the flavonoid biosynthesis pathway, which is essential for the development of color and flavor in fruits. Here, we show that Fra a proteins bind natural flavonoids with different selectivity and affinities in the low μm range. The structural analysis of Fra a 1 E and a Fra a 3-catechin complex indicates that loops L3, L5, and L7 surrounding the ligand-binding cavity show significant flexibility in the apo forms but close over the ligand in the Fra a 3-catechin complex. Our findings provide mechanistic insight on the function of Fra a proteins and suggest that PR-10 proteins, which are widespread in plants, may play a role in the control of secondary metabolic pathways by binding to metabolic intermediates. 相似文献
7.
8.
Purification and characterization of a human recombinant T-cell protein-tyrosine-phosphatase from a baculovirus expression system. 总被引:2,自引:0,他引:2
N F Zander J A Lorenzen D E Cool N K Tonks G Daum E G Krebs E H Fischer 《Biochemistry》1991,30(28):6964-6970
A 48-kDa human T-cell protein-tyrosine-phosphatase (TC.PTPase) and a truncated form missing an 11-kDa C-terminal segment (TC delta C11.PTPase) were expressed by using the baculovirus system and characterized after extensive purification. The full-length PTPase was restricted to the particulate fraction of the cells from which it could be released by a combination of salt and detergent. The enzyme was entirely specific for phosphotyrosine residues. It displayed a low level of activity toward phosphorylated, reduced, carboxamidomethylated, and maleylated lysozyme (RCML), but was 12 times more active toward phosphorylated myelin basic protein (MBP). By contrast, the 37-kDa form localized in the soluble fraction, and its activity toward RCML was 5 times higher than that observed with MBP. The autophosphorylated cytoplasmic domain of the EGF receptor served as substrate for both enzymes. Limited proteolysis of either protein gave rise to a 33-kDa fragment displaying the substrate specificity of the truncated form. These data lend further support to the view that the C-terminal segment of the T-cell PTPase serves a regulatory function, playing an important role in the localization and substrate specificity of the enzyme. 相似文献
9.
Roland T. Ullrich Thomas Zander Bernd Neumaier Mirjam Koker Takeshi Shimamura Yannic Waerzeggers Christa L. Borgman Samir Tawadros Hongfeng Li Martin L. Sos Heiko Backes Geoffrey I. Shapiro Jürgen Wolf Andreas H. Jacobs Roman K. Thomas Alexandra Winkeler 《PloS one》2008,3(12)
Background
Inhibition of the epidermal growth factor receptor (EGFR) has shown clinical success in patients with advanced non-small cell lung cancer (NSCLC). Somatic mutations of EGFR were found in lung adenocarcinoma that lead to exquisite dependency on EGFR signaling; thus patients with EGFR-mutant tumors are at high chance of response to EGFR inhibitors. However, imaging approaches affording early identification of tumor response in EGFR-dependent carcinomas have so far been lacking.Methodology/Principal Findings
We performed a systematic comparison of 3′-Deoxy-3′-[18F]-fluoro-L-thymidine ([18F]FLT) and 2-[18F]-fluoro-2-deoxy-D-glucose ([18F]FDG) positron emission tomography (PET) for their potential to identify response to EGFR inhibitors in a model of EGFR-dependent lung cancer early after treatment initiation. While erlotinib-sensitive tumors exhibited a striking and reproducible decrease in [18F]FLT uptake after only two days of treatment, [18F]FDG PET based imaging revealed no consistent reduction in tumor glucose uptake. In sensitive tumors, a decrease in [18F]FLT PET but not [18F]FDG PET uptake correlated with cell cycle arrest and induction of apoptosis. The reduction in [18F]FLT PET signal at day 2 translated into dramatic tumor shrinkage four days later. Furthermore, the specificity of our results is confirmed by the complete lack of [18F]FLT PET response of tumors expressing the T790M erlotinib resistance mutation of EGFR.Conclusions
[18F]FLT PET enables robust identification of erlotinib response in EGFR-dependent tumors at a very early stage. [18F]FLT PET imaging may represent an appropriate method for early prediction of response to EGFR TKI treatment in patients with NSCLC. 相似文献10.
David W. Thieltges Per‐Arne Amundsen Ryan F. Hechinger Pieter T. J. Johnson Kevin D. Lafferty Kim N. Mouritsen Daniel L. Preston Karsten Reise C. Dieter Zander Robert Poulin 《Oikos》2013,122(10):1473-1482
While the recent inclusion of parasites into food‐web studies has highlighted the role of parasites as consumers, there is accumulating evidence that parasites can also serve as prey for predators. Here we investigated empirical patterns of predation on parasites and their relationships with parasite transmission in eight topological food webs representing marine and freshwater ecosystems. Within each food web, we examined links in the typical predator–prey sub web as well as the predator–parasite sub web, i.e. the quadrant of the food web indicating which predators eat parasites. Most predator– parasite links represented ‘concomitant predation’ (consumption and death of a parasite along with the prey/host; 58–72%), followed by ‘trophic transmission’ (predator feeds on infected prey and becomes infected; 8–32%) and predation on free‐living parasite life‐cycle stages (4–30%). Parasite life‐cycle stages had, on average, between 4.2 and 14.2 predators. Among the food webs, as predator richness increased, the number of links exploited by trophically transmitted parasites increased at about the same rate as did the number of links where these stages serve as prey. On the whole, our analyses suggest that predation on parasites has important consequences for both predators and parasites, and food web structure. Because our analysis is solely based on topological webs, determining the strength of these interactions is a promising avenue for future research. 相似文献