The Effect of Gases on the Structural Integrity of Ice that Forms when Water and Cryoprotective Solutions Are Frozen: An Optical-Microscope Study

Biophysics - The effects of air, helium, argon, xenon, and sulfur hexafluoride on the integrity of the ice structure formed when water and a cryoprotective solutions were frozen in the temperature...     

The Effect of Helium on Cryopreservation of HeLa and L929 Cells

The effect of helium on cell survival during cryopreservation was studied using the HeLa and L929 cell lines. Cell suspensions were incubated in an atmosphere of helium, nitrogen, or air and frozen in the presence or absence of glycerol as a cryoprotectant. After thawing, the cell viability was evaluated by the Trypan Blue exclusion test and culture development for 18 h. Helium was found to provide better preservation of cell suspensions compared with nitrogen and air. The positive effect of helium was the greatest in the case of freezing without cryoprotectants (the HeLa cell survival increased by a factor of 1.5–2) and somewhat lower in the case of freezing in the presence of low glycerol concentrations (the L929 cell survival increased by a factor of 1.2–1.5 in the presence of 3% glycerol). Use of helium for cell suspensions may improve cryopreservation methods by making it possible to reduce the concentrations of conventional cryoprotectants, which are generally highly toxic and undesirable to use for cryopreservation of biological material for medical applications.     

Fetal Serum in Combination with 5% Dimethyl Sulfoxide Efficiently Protects the Human Gut Microbiota during Cryopreservation in Liquid Nitrogen

Biophysics - Due to advances in medical technologies, it is an important problem to develop methods that allow safe and efficient long-term preservation of the human gut microbiota without...     

The Efficiency of the Preservation of Human Gut Microbiota in Liquid Nitrogen Depending on the Composition of the Cryoprotective Medium

Biophysics - A comparative analysis of the survival rate of the human gut microbiota after low-temperature preservation under the protection of penetrating (dimethylsulfoxide and glycerol),...