Biocontrol of wilt disease complex of pea caused by the root-knot nematode
Meloidogyne incognita and
Fusarium oxysporum f. sp.
pisi was studied on pea (
Pisum sativum L.) using plant growth-promoting rhizobacterium
Pseudomonas fluorescens and root nodule bacterium
Rhizobium sp. Inoculation of
M. incognita and
F.oxysporum alone caused significant reductions in plant growth over un-inoculated control. Reduction in plant growth caused by
M. incognita was statistically equal to that caused by
F. oxysporum. Inoculation of
M. incognita plus
F. oxysporum together caused a greater reduction in plant growth than the sum of damage caused by these pathogens singly. Inoculation of
P. fluorescens and
Rhizobium sp. individually or both together increased plant growth in pathogen inoculated and un-inoculated plants. Inoculation of
P. fluorescens to pathogen-inoculated plants caused a greater increase in plant growth than caused by
Rhizobium sp. Application of
Rhizobium plus
P. fluorescens caused a greater increase in plant growth than caused by each of them singly. Inoculation of
P.fluorescens caused higher reduction in galling and nematode multiplication than caused by
Rhizobium sp. Use of
Rhizobium plus
P. fluorescens caused higher reduction in galling and nematode multiplication than their individual inoculation. Plants inoculated with both pathogens plus
Rhizobium showed less nodulation than plants inoculated with single pathogen plus
Rhizobium. Inoculation of
Rhizobium plus
P. fluorescens resulted in higher root-nodulation than inoculated only with
Rhizobium. Wilting indices were 4 and 5, respectively, when plants were inoculated with
F. oxysporum and
F. oxysporum plus
M. incognita. Wilting indices were reduced maximum to 1 and 2, respectively, when plants inoculated with
F.oxysporum and plants with both pathogens were treated with
P. fluorescens plus
Rhizobium.
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