Quantitative phosphoproteomic analyses provide evidence for extensive phosphorylation of regulatory proteins in the rhizobia–legume symbiosis

Plant Molecular Biology - PsLecRLK overexpression in rice provides tolerance against salinity stress and cause upregulation of SOS1 pathway genes, which are responsible for extrusion of excess Na+...     

Fine mapping of an Arabidopsis thaliana male sterile mutant EC2-157

An Arabidopsis thaliana male sterile mutant EC2-157 has been isolated using an EMS mutagenesis strategy. Genetic analysis indicated that it was controlled by a single recessive gene called ms157. No pollen grains have been observed in mutant anthers. ms157 Has been mapped to a region of 74kb located in BAC clone T6K22 on chromosome IV using a map-based cloning strategy. As no male sterile genes have been reported in this region, ms157 could be a novel gene related to fertility. The further molecular cloning and functional analysis on this gene should facilitate our understanding of A. thaliana another development. __________ Translated from Journal of Shanghai Normal University (Natural Sciences), 2005, 34(1): 58–63 [译自: 上海师范大学学报 (自然科学版), 2005, 34(1): 58–63]     

Genomic,molecular evolution,and expression analysis of NOX genes in soybean (Glycine max)

Reactive oxygen species (ROS) are versatile signaling molecules in sensing stresses and play critical roles in signaling and development. Plasma membrane NADPH oxidases (NOXs) are key producers of ROS, and play important roles in the regulation of plant-pathogen interactions. Here, we performed a comprehensive analysis of the NOX gene family in the soybean genome (Glycine max) and 17 NOX (GmNOX) genes were identified. Structural analysis revealed that the GmNOX proteins in soybean were as conserved as those in other plants. 8 duplicated gene pairs were formed by a Glycine-specific whole-genome duplication (WGD) event approximately 13 million years ago (Mya). The Ka/Ks ratios of GmNOX genes ranged from 0.04 to 0.28, suggesting that the GmNOX family had undergone purifying selection in soybean. Gene expression patterns showed different expression of these duplicate genes, suggesting that the GmNOXs were retained by substantial subfunctionalization during the soybean evolutionary processes. Subsequently, the expression of GmNOXs in response to drought and phytohormones were characterized via qPCR. Importantly, four GmNOXs showed strong expression in nodules, pointing to their probable involvement in nodulation. Thus, our results shed light on the evolutionary history of this family in soybean and contribute to the functional characterization of GmNOX genes in soybean.     

Fine mapping of an Arabidopsis thaliana male sterile mutant EC2-157

An Arabidopsis thaliana male sterile mutant EC2-157 has been isolated using an EMS mutagenesis strategy.Genetic analysis indicated that it was controlled by a single recessive gene called ms157.No pollen grains have been observed in mutant anthers.ms157 Has been mapped to a region of 74 kb located in BAC clone T6K22 on chromosome Ⅳ using a map-based cloning strategy.As no male sterile genes have been reported in this region.ms157 could be a novel gene related to fertility.The further molecular cloning and functional analysis on this gene should facilitate our understanding of A.thaliana anther development.     

农林复合系统中灌木篱墙对异色瓢虫种群分布的影响

以北京大兴农区农林复合系统为对象,围绕灌木篱墙(沙棘绿篱和杨树萌条带)上和麦田中的主要捕食性天敌——异色瓢虫对栖息地的选择趋向及灌木篱墙对异色瓢虫种群分布的影响进行了研究。结果表明:(1)小麦生长前期(返青期至抽穗期或扬花期)灌木篱墙上异色瓢虫种群密度是麦田的3.25—9.57倍,是异色瓢虫由越冬地向麦田过渡前的主要栖息地,是农林复合系统中异色瓢虫种群建立的"种库";(2)异色瓢虫对栖息生境有较强的选择性,明显趋向于蚜虫密集分布的生境,小麦生长前期趋向于灌木篱墙,小麦生后期(扬花期或灌浆期至完熟期)趋向于麦田,整个小麦生长期内异色瓢虫种群在不同生境斑块间存在明显的移动现象;(3)麦收后,邻近麦田的杨树萌条带上异色瓢虫种群密度相比于完熟期增加了42%,为异色瓢虫提供了重要的庇护场所。     

农林复合环境大草蛉生境选择取向及天敌效能

大草蛉(Chrysopa pallens Rambur)是华北农区优势天敌昆虫之一。华北农区农林复合系统中作物农田、林带所组成的农林生境,就其中的大草蛉对栖息环境、产卵地点、越冬场所等不同生境的选择取向及天敌追随效能展开了多角度研究,结果表明:(1)大草蛉成虫对作物蚜虫具有明显的追随效应,其幼虫对作物蚜虫的空间跟随作用显著,且在不同空间层次均有控制作物蚜虫的表现(空间重叠度指数:P_(幼空间)0.65);大草蛉的采食虫态期与蚜虫的发生期同步(时间重叠度指数:P_(幼空间)0.65)。(2)紧邻农田的林带环境是秋收后大草蛉主要的越冬场所(差异显著性比较:P=0.0010.05,α=0.05),对维系大草蛉在农林生境中的种群延续具有重要作用。(3)大草蛉在林带内的树皮裂缝、地表土层、枯枝落叶下结茧化蛹越冬,并对以上3个地点不存在明显选择性(差异显著性比较:P=0.4660.05,α=0.05);(4)大草蛉在栖息生境中具有季节移动性和偏好选择性双重特点:夏秋两季作物生长期内大草蛉从林带迁飞往农田,偏好在大豆、花生田中栖息(LSD法:P=0.0050.01,α=0.01);农作物秋收后及冬春两季大草蛉栖居于林带中,偏好在杨树萌条林内栖息(LSD法:P=0.0020.01,α=0.01);(5)大草蛉对产卵地点具有选择性,因生境不同表现出一定差异性:在农田生境,大草蛉产卵时节贯穿6至9月,且偏好于花生与玉米田(LSD法:P=0.0070.01,α=0.01);在林带生境,杨树杂木林内具有更高密度卵量(LSD法:P=0.0380.05,α=0.05),产卵时节集中在5至7月。     

退耕还林对地面蜘蛛种群的影响

为探究退耕还林对地面蜘蛛群落的影响,分别于2014和2015年3月—9月期间,采用陷阱法对北京市顺义区南彩镇退耕还林已2a和3a的林带及相邻原非作物生境林带中地面蜘蛛的活动密度进行了系统调查和分析。结果,2014年在调查区域共采集地面蜘蛛1465头,隶属8科18属32种,2015年共收集地面蜘蛛2186头,隶属10科25属45种;退耕还林以后蜘蛛类群发生改变,地面蜘蛛丰富度增加,而部分农田蜘蛛消失;2014年原非作物生境林带中地面蜘蛛的多样性指数、丰富度都极显著高于退耕还林林带(P0.01),均匀性指数不存在显著差异,2015年两个林带的多样性指数仍存在极显著差异(P0.01),丰富度存在显著差异(P0.05),均匀性指数之间不存在显著差异;退耕还林以后第3年新建林带和原非作物生境林带中地面蜘蛛的活动密度随时间的变化趋势相近。研究结果表明:顺义地区退耕还林工程实施3a以后,新建人工林仍处于生境重建阶段,未完全达到稳定状态,退耕还林以后第3年新建林带中地面蜘蛛群落的稳定性高于第2年。退耕还林通过改变原有农田生态系统的植被种类、结构、人为管理等方式,使原有农田生态系统发生了根本性的改变。持续研究退耕还林对地面蜘蛛的类群变化的影响对生境受到干扰后生物群落变化过程的认识有理论意义。