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1.
Auxin-induced mRNA species in tobacco cell cultures 总被引:6,自引:0,他引:6
E. J. van der Zaal J. Memelink A. M. Mennes A. Quint K. R. Libbenga 《Plant molecular biology》1987,10(2):145-157
2.
When 2,4-dichlorophenoxyacetic acid (2,4-D)-dependent tobacco cell suspensions, one normal and one transformed by Agrobacterium tumefaciens, were subcultured on hormone-lacking medium the stationary phase of the cell cycle was reached earlier than on medium containing 2,4-D. Addition of the auxin 2,4-D could restore cell division activity within 10–12 h for the most rapidly reacting cell line. The cell-division response was characterized as being auxin-specific and optimal with 2,4-D at 2.2 10-6 M. Although the cell lines used showed different characteristics, both reacted with a rapid increase in at least three mRNA species within 1 or 2 h after 2,4-D application. Two, 2,4-D-induced protein spots, seen after in-vitro translation, had the same characteristics (MWs 35 kilodaltons (kDa) and 25 kDa with isoelectric points of 7.1 and 6.3, respectively) in both cell lines. Water-treated controls did not show alterations in the translatable mRNA populations. This indicates that the accumulation of the corresponding mRNAs is an early hormone-induced event. Since cell division is the only measurable reaction found after auxin application, cell systems as described here offer excellent possibilities for studying early auxin-induced changes at the molecular level preceding mitosis.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- IAA
indole-3-acetic acid
- kDa
kilodalton 相似文献
3.
Promoters of auxin-induced genes from tobacco can lead to auxin-inducible and root tip-specific expression 总被引:13,自引:0,他引:13
E. J. van der Zaal F. N. J. Droog C. J. M. Boot L. A. M. Hensgens J. H. C. Hoge R. A. Schilperoort K. R. Libbenga 《Plant molecular biology》1991,16(6):983-998
In previous studies we have identified several mRNAs which accumulate after addition of 2,4-dichlorophenoxyacetic-acid (2,4-D) to auxin-starved tobacco cells [45, 46]. The mRNAs corresponding to cDNA clone pCNT103 were found to accumulate transiently prior to the cell division response due to auxin treatment. In this study we determined the sequences of three 103-like cDNAs and two 103-like genes, GNT1 and GNT35. To further study the regulation of the expression of these genes their 5 regions were translationally fused with the -D-glucuronidase reporter gene (GUS). The GNT1 5 region led to GUS expression only in the root tips of transgenic plants. By using transgenic hairy-root cultures and transformed cell suspension cultures it was shown that the 5 regions of both GNT1 and GNT35 lead to 2,4-D-inducible expression of GUS activity. The homology of the 103-like genes with other auxin-regulated genes is evaluated.Department of Plant Molecular Biology, Leiden University 相似文献
4.
5.
Facilitation in plant communities: the past, the present, and the future 总被引:24,自引:11,他引:24
Rob W. Brooker Fernando T. Maestre Ragan M. Callaway Christopher L. Lortie Lohengrin A. Cavieres Georges Kunstler Pierre Liancourt Katja Tielbörger Justin M. J. Travis Fabien Anthelme Cristina Armas Lluis Coll Emmanuel Corcket Sylvain Delzon Estelle Forey Zaal Kikvidze Johan Olofsson Francisco Pugnaire Constanza L. Quiroz Patrick Saccone Katja Schiffers Merav Seifan Blaize Touzard Richard Michalet 《Journal of Ecology》2008,96(1):18-34
6.
Sarah Oddoux Kristien J. Zaal Victoria Tate Aster Kenea Shuktika A. Nandkeolyar Ericka Reid Wenhua Liu Evelyn Ralston 《The Journal of cell biology》2013,203(2):205-213
Skeletal muscle microtubules (MTs) form a nonclassic grid-like network, which has so far been documented in static images only. We have now observed and analyzed dynamics of GFP constructs of MT and Golgi markers in single live fibers and in the whole mouse muscle in vivo. Using confocal, intravital, and superresolution microscopy, we find that muscle MTs are dynamic, growing at the typical speed of ∼9 µm/min, and forming small bundles that build a durable network. We also show that static Golgi elements, associated with the MT-organizing center proteins γ-tubulin and pericentrin, are major sites of muscle MT nucleation, in addition to the previously identified sites (i.e., nuclear membranes). These data give us a framework for understanding how muscle MTs organize and how they contribute to the pathology of muscle diseases such as Duchenne muscular dystrophy. 相似文献
7.
Zenderland Jasmine Hart Robbie Bussmann Rainer W. Paniagua Zambrana Narel Y. Sikharulidze Shalva Kikvidze Zaal Kikodze David Tchelidze David Khutsishvili Manana Batsatsashvili Ketevan 《Economic botany》2019,73(3):293-303
Economic Botany - The Use of “Use Value”: Quantifying Importance in Ethnobotany. Use value (UV) is an index widely used to quantify the relative importance of useful plants. It combines... 相似文献
8.
Overexpression of a novel Arabidopsis gene related to putative zinc-transporter genes from animals can lead to enhanced zinc resistance and accumulation 总被引:31,自引:0,他引:31
van der Zaal BJ Neuteboom LW Pinas JE Chardonnens AN Schat H Verkleij JA Hooykaas PJ 《Plant physiology》1999,119(3):1047-1055
We describe the isolation of an Arabidopsis gene that is closely related to the animal ZnT genes (Zn transporter). The protein encoded by the ZAT (Zn transporter of Arabidopsis thaliana) gene has 398 amino acid residues and is predicted to have six membrane-spanning domains. To obtain evidence for the postulated function of the Arabidopsis gene, transgenic plants with the ZAT coding sequence under control of the cauliflower mosaic virus 35S promoter were analyzed. Plants obtained with ZAT in the sense orientation exhibited enhanced Zn resistance and strongly increased Zn content in the roots under high Zn exposure. Antisense mRNA-producing plants were viable, with a wild-type level of Zn resistance and content, like plants expressing a truncated coding sequence lacking the C-terminal cytoplasmic domain of the protein. The availability of ZAT can lead to a better understanding of the mechanism of Zn homeostasis and resistance in plants. 相似文献
9.
Measuring the number of co-dominants in ecological communities 总被引:1,自引:0,他引:1
We suggest a concept that allows the objective determination of the number of co-dominants in a community. We define co-dominants as a subset of species that are more abundant and more uniformly distributed than other species in a given sample. We compare the sample with a model community and use Simpsons diversity index to estimate the apparent number of co-dominants. Dominant species determined in this way are responsible for 70–90% of the total measure of abundance in the sample. The statistical significance of the apparent number of co-dominants may be assessed by a randomization test. 相似文献
10.
Christian Schöb Peter M. Kammer Zaal Kikvidze Philippe Choler Stefanie Von Felten Heinz Veit 《Oikos》2010,119(10):1571-1580
Alpine snowbeds are habitats where the major limiting factors for plant growth are herbivory and a small time window for growth due to late snowmelt. Despite these limitations, snowbed vegetation usually forms a dense carpet of palatable plants due to favourable abiotic conditions for plant growth within the short growing season. These environmental characteristics make snowbeds particularly interesting to study the interplay of facilitation and competition. We hypothesised an interplay between resource competition and facilitation against herbivory. Further, we investigated whether these predicted neighbour effects were species‐specific and/or dependent on ontogeny, and whether the balance of positive and negative plant–plant interactions shifted along a snowmelt gradient. We determined the neighbour effects by means of neighbour removal experiments along the snowmelt gradient, and linear mixed model analyses. The results showed that the effects of neighbour removal were weak but generally consistent among species and snowmelt dates, and depended on whether biomass production or survival was considered. Higher total biomass and increased fruiting in removal plots indicated that plants competed for nutrients, water, and light, thereby supporting the hypothesis of prevailing competition for resources in snowbeds. However, the presence of neighbours reduced herbivory and thereby also facilitated survival. For plant growth the facilitative effects against herbivores in snowbeds counterbalanced competition for resources, leading to a weak negative net effect. Overall the neighbour effects were not species‐specific and did not change with snowmelt date. Our finding of counterbalancing effects of competition and facilitation within a plant community is of special theoretical value for species distribution models and can explain the success of models that give primary importance to abiotic factors and tend to overlook interrelations between biotic and abiotic effects on plants. 相似文献