Comparative evaluation of the action of prostanoid inhibitors on uterine contractile function

Indomethacin and substance BW-755C in experiments on isolated myometrium striae of pregnant white rats exert an inhibiting effect on the contractile uterus function due to inhibition of cyclooxygenase or lipoxygenase ways of the arachidonic acid transformation. Prostaglandin F2 alpha is sensitive to functioning of the cyclooxygenase and lipoxygenase ways of the arachidonic acid transformation, while oxytocin--only lipoxygenase one. Conclusions rest on results from multiparametric analysis of the contractile uterus function suggested by authors and confirmed by the pattern recognition method--the Karunen-Loev orthogonal decomposition.     

The evaluation and prognosis of the psychophysiological status of a human operator

In experiments on 56 healthy subjects (18-20 years old) the quality of their activity was determined during compensatory watching the mark at complicating regimes of work. Depending on the difficulty of the task five groups of subjects were singled out with optimum working capacity in one of four working conditions: normal, ordinary and strenuous work, model of stress situation. It is established that the change of the number of significant correlative connections between main parameters of psychophysiological state of man-operator reflects the condition of his functional systems. On the basis of computation of total range of organization values of both R-R intervals of the ECG and duration of expiration, the success of the man-operator work in complex conditions of activity is predicted.     

Biosynthesis and use of coproporphyrins and uroporphyrins and their metal complexes in immune analysis and diagnostic methods]

Methods of synthesis of coproporphyrin and uroporphyrin by using bacteria of the genus Arthrobacter are proposed. Metal complexes of coproporphyrin and uroporphyrin with Pt, Pd, and Zn were synthesized. Their structures were identified by spectrophotometry, IR spectrometry, 1H-NMR, mass spectrometry, and HPLC. Data showing the possibility to use coproporphyrin III-metal complexes as luminophores for fluorescence detection of tumors. The current and prospective uses of metal complexes of water-soluble natural porphyrins in advanced immunofluorescence assays are discussed.     

Dynamics of the spectral-coherent characteristics of the human EEG in healthy subjects and brain pathology

EEGs of 15 healthy subjects and 30 patients in early period after surgical ablation of tumours with basal localization, were investigated by means of monitor "Neuro-1" which allows to obtain in continuous regime characteristics of EEG dynamics according to power spectra and reveal the characteristics of intercentral relations of electrical brain processes by coherence and phase shifts. In healthy subjects in the period of transition from wakefulness to drowsiness highly coherent beta-rhythm (16-18 Hz) was revealed, preceeding typical picture of drowsiness and sleep. Study of the patients shows that certain characteristics of coherence and phasic shifts have an important prognostic value. Conclusion is made that for estimation of the functional state of healthy subjects during transition from wakefulness to drowsiness and of patients in early postoperative period the data on spectra of coherence are most informative.     

Neurospecific and neuron-specific enolase levels in human nerve tissue at early stages of embryogenesis

Neurospecific and neuro-nonspecific isozymes of glycolytic enzyme enolase known to be molecular markers of neuronal and glial cells respectively were isolated from human brain. Using immunoenzyme assay, the content of these proteins was measured in the brain of 7-13-week human embryos and adult subjects. The content of both isoenzymes gradually increases attaining constant level in 10-13-week embryos. Relative content of neuro-nonspecific enolase in the brain of 10-13-week embryos is 1 1/2 times higher than that in adults, whereas the content of neurospecific enolase amounts only to 2% of its level in the adult brain.     

Transformation of erythrocytes and their ultrastructure with respect to aging

Transformation and ultrastructure of erythrocytes have been investigated in 58 white non-inbred rats on the 1st, 2nd, 3d days after birth and at the age of 1.5, 3, 8, 20 months. The transformation index of the erythrocytes drops by the 8th month of life and further it rises in old animals. In newborn rats echino-poikiloid forms predominate; they are mainly presented as reticulocytes with remnants of nuclei, mitochondria and other organells. During first three days of life cells of the erythroid line actively free themselves from the nucleus by means of its pyknosis and chromatinolysis. In newborn rats erythrocytes have folds of plasmolemma of linear and spotted form on their surface; their number sharply decreases in mature animals. The least changes in form and ultrastructure of erythrocytes are noticed in 3-8-month-old animals.