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1.
T. V. Yuzbashev T. V. Vybornaya A. S. Larina I. T. Gvilava N. E. Voyushina S. S. Mokrova E. Yu. Yuzbasheva I. V. Manukhov S. P. Sineoky V. G. Debabov 《Applied Biochemistry and Microbiology》2013,49(9):723-742
The modification of Escherichia coli K-12 metabolism leading to threonine overproduction is the most studied system in synthetic biology that has been used to elaborate the majority of the currently known approaches to constructing microbial producers. They include optimization of biosynthesis through search for rate-limiting stages, modification of substrate and product transport, elimination of side metabolic pathways and degradation systems, reinforcement of the regeneration of coenzymes that are required for product biosynthesis, and exclusion of futile cycles and metabolic pathways with low energy efficiency. Extensive research in functional genomics made it possible to selectively remove the “unnecessary genes,” the functions of which are useless for producing a strain or adversely affect its properties. In total, using various approaches to designing threonine-producing strains, over 150 genome loci that affect more than 30% genes in E. coli were directly modified, thus providing interesting data for researchers in the field of microbial synthesis, as well as in related biological sciences. This review is dedicated to the assessment of genetic engineering modifications in E. coli metabolism (primarily, on the basis of modern patent literature) that ensure threonine overproduction. 相似文献
2.
Melanoma is the most lethal cutaneous cancer with a highly aggressive and metastatic phenotype. While recent genetic and epigenetic studies have shed new insights into the mechanism of melanoma development, the involvement of regulatory non‐coding RNAs remain unclear. Long non‐coding RNAs (lncRNAs) are a group of endogenous non‐protein‐coding RNAs with the capacity to regulate gene expression at multiple levels. Recent evidences have shown that lncRNAs can regulate many cellular processes, such as cell proliferation, differentiation, migration and invasion. In the melanoma, deregulation of a number of lncRNAs, such as HOTAIR, MALAT1, BANCR, ANRIL, SPRY‐IT1 and SAMMSON, have been reported. Our review summarizes the functional role of lncRNAs in melanoma and their potential clinical application for diagnosis, prognostication and treatment. 相似文献
3.
Priscila R Moreira Marcos A Maioli Hyllana CD Medeiros Marieli Guelfi Flávia TV Pereira Fábio E Mingatto 《Biological research》2014,47(1)
Background
The liver is an important organ for its ability to transform xenobiotics, making the liver tissue a prime target for toxic substances. The carotenoid bixin present in annatto is an antioxidant that can protect cells and tissues against the deleterious effects of free radicals. In this study, we evaluated the protective effect of bixin on liver damage induced by carbon tetrachloride (CCl4) in rats.Results
The animals were divided into four groups with six rats in each group. CCl4 (0.125 mL kg-1 body wt.) was injected intraperitoneally, and bixin (5.0 mg kg-1 body wt.) was given by gavage 7 days before the CCl4 injection. Bixin prevented the liver damage caused by CCl4, as noted by the significant decrease in serum aminotransferases release. Bixin protected the liver against the oxidizing effects of CCl4 by preventing a decrease in glutathione reductase activity and the levels of reduced glutathione and NADPH. The peroxidation of membrane lipids and histopathological damage of the liver was significantly prevented by bixin treatment.Conclusion
Therefore, we can conclude that the protective effect of bixin against hepatotoxicity induced by CCl4 is related to the antioxidant activity of the compound. 相似文献4.
E. Yu. Yuzbasheva T. V. Yuzbashev T. K. Konstantinova I. A. Laptev N. I. Perkovskaya S. P. Sineokii 《Applied Biochemistry and Microbiology》2011,47(8):744-753
The closest homologue of the Saccharomyces cerevisiae Flo1p cell wall protein was detected in Yarrowia lipolytica yeast (YALI0C09031p) by the method of genomic analysis, and capacity of its N- and C-domains to expose the Lip2 lipase on
the cell surface was studied. The efficient fixation of the enzyme on the Y. lipolytica cell wall surface was demonstrated. The activity of the cell-bound lipase was 9170 and 3200 units per 1 g of dry solid matter
when using N- and C-domains of the cell wall protein, respectively. At the same time, in the case of immobilization using
the N-domain, approximately 30% of the total lipase activity was detected in the culture medium, whereas when using C-domain
of the cell wall protein YALI0C09031p, practically all lipase was in the immobilized state. Obtained values of the level of
the cell-bound lipase activity considerably exceed previously published data opening a prospect for new technological solutions
which meet industrial needs. 相似文献
5.
Tigran V. Yuzbashev Evgeniya Y. Yuzbasheva Tatiana I. Sobolevskaya Ivan A. Laptev Tatiana V. Vybornaya Anna S. Larina Kazuhiko Matsui Keita Fukui Sergey P. Sineoky 《Biotechnology and bioengineering》2010,107(4):673-682
Biotechnological production of weak organic acids such as succinic acid is most economically advantageous when carried out at low pH. Among naturally occurring microorganisms, several bacterial strains are known to produce considerable amounts of succinic acid under anaerobic conditions but they are inefficient in performing the low‐pH fermentation due to their physiological properties. We have proposed therefore a new strategy for construction of an aerobic eukaryotic producer on the basis of the yeast Yarrowia lipolytica with a deletion in the gene coding one of succinate dehydrogenase subunits. Firstly, an original in vitro mutagenesis‐based approach was proposed to construct strains with Ts mutations in the Y. lipolytica SDH1 gene. These mutants were used to optimize the composition of the media for selection of transformants with the deletion in the Y. lipolytica SDH2 gene. Surprisingly, the defects of each succinate dehydrogenase subunit prevented the growth on glucose but the mutant strains grew on glycerol and produced succinate in the presence of the buffering agent CaCO3. Subsequent selection of the strain with deleted SDH2 gene for increased viability allowed us to obtain a strain capable of accumulating succinate at the level of more than 45 g L?1 in shaking flasks with buffering and more than 17 g L?1 without buffering. The possible effect of the mutations on the utilization of different substrates and perspectives of constructing an industrial producer is discussed. Biotechnol. Bioeng. 2010;107:673–682. © 2010 Wiley Periodicals, Inc. 相似文献
6.
Evgeniya Y. Yuzbasheva Elizaveta B. Mostova Natalia I. Andreeva Tigran V. Yuzbashev Alexander S. Fedorov Irina A. Konova Sergey P. Sineoky 《Biotechnology and bioengineering》2018,115(2):433-443
7.
Yuzbashev TV Yuzbasheva EY Laptev IA Sobolevskaya TI Vybornaya TV Larina AS Gvilava IT Antonova SV Sineoky SP 《Bioengineered bugs》2011,2(2):115-119
Bio-based succinate is still a matter of special emphasis in biotechnology and adjacent research areas. The vast majority of natural and engineered producers are bacterial strains that accumulate succinate under anaerobic conditions. Recently, we succeeded in obtaining an aerobic yeast strain capable of producing succinic acid at low pH. Herein, we discuss some difficulties and advantages of microbial pathways producing "succinic acid" rather than "succinate." It was concluded that the peculiar properties of the constructed yeast strain could be clarified in view of a distorted energy balance. There is evidence that in an acidic environment, the majority of the cellular energy available as ATP will be spent for proton and anion efflux. The decreased ATP:ADP ratio could essentially reduce the growth rate or even completely inhibit growth. In the same way, the preference of this elaborated strain for certain carbon sources could be explained in terms of energy balance. Nevertheless, the opportunity to exclude alkali and mineral acid waste from microbial succinate production seems environmentally friendly and cost-effective. 相似文献
8.
Yuzbashev TV Yuzbasheva EY Vibornaya TV Sobolevskaya TI Laptev IA Gavrikov AV Sineoky SP 《Protein expression and purification》2012,82(1):83-89
The gene encoding Rhizopus oryzae lipase (ROL) was expressed in the non-conventional yeast Yarrowia lipolytica under the control of the strong inducible XPR2 gene promoter. The effects of three different preprosequence variants were examined: a preprosequence of the Y. lipolytica alkaline extracellular protease (AEP) encoded by XPR2, the native preprosequence of ROL, and a hybrid variant of the presequence of AEP and the prosequence of ROL. Lipase production was highest (7.6 U/mL) with the hybrid prepropeptide. The recombinant protein was purified by ion-exchange chromatography. The ROL included 28 amino acids of the C-terminal region of the prosequence, indicating that proteolytic cleavage occurred below the KR site through the activity of the Kex2-like endoprotease. The optimum temperature for recombinant lipase activity was between 30 and 40 °C, and the optimum pH was 7.5. The enzyme was shown not to be glycosylated. Furthermore, recombinant ROL exhibited greater thermostability than previously reported, with the enzyme retaining 64% of its hydrolytic activity after 30 min of incubation at 55 °C. 相似文献
9.
Metabolic evolution and 13C flux analysis of a succinate dehydrogenase deficient strain of Yarrowia lipolytica 下载免费PDF全文
10.
INTRODUCTIONAsearlyasin1948wehavefr8CtionatedisolatednucleifromnormalandtumorcellsbyextractionwithiMNaCIanddilutealkali[1].Thenuclearresiduewasthenstudiedmorethoroughly[2,3].Lateron,sillillarproteinousnuclearresidueswereisolatedbyotherworkers[46]andasstud… 相似文献