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Pimsiri Danphitsanuparn Panitch Boonsnongcheep Thanaphol Boriboonkaset Yupyn Chintapakorn Sompop Prathanturarug 《Plant Cell, Tissue and Organ Culture》2012,111(3):315-322
Using several explants of Pueraria candollei Grah. ex Benth. var. candollei and two strains of Agrobacterium rhizogenes (ATCC 15834 and 43057), hairy root cultures were established. Including 100???M acetosyringone in the culture medium enhanced frequency of hairy root induction by up to 58?%. Subsequently, effects of inoculum size (IS) and temperature on growth and production of isoflavonoids in hairy roots were determined. Conditions of 1?%?IS and 32?°C promoted the highest accumulation of total isoflavonoid content, up to 31.0?±?22.6?mg/g dry weight (DW), in hairy roots. Moreover, culture of hairy roots at 32?°C decreased browning of hairy roots. Furthermore, this temperature promoted accumulation of the secondary metabolite daidzein; whereas, hairy root cultures at the stationary phase accumulated higher amounts of the isoflavonoid puerarin rather than daidzein. 相似文献
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Panitch Boonsnongcheep Sirintra Korsangruang Noppamas Soonthornchareonnon Yupyn Chintapakorn Promchit Saralamp Sompop Prathanturarug 《Plant Cell, Tissue and Organ Culture》2010,101(2):119-126
We established cell suspension cultures derived from leaf, stem, and root calli of Pueraria candollei var. candollei and P. candollei var. mirifica using liquid Murashige and Skoog (MS) medium supplemented with 0.56 μM 6-benzyladenine (BA) and 4.52 μM 2,4-dichlorophenoxyacetic
acid (2,4-D). Growth of the cell suspension cultures progressed to the stationary phase within 15–24 days. Methanolic extracts
of cell suspension cultures of both varieties of P. candollei were analyzed using a validated HPLC protocol. All cell lines derived from leaf, stem, and root explants produced four major
isoflavonoids: daidzein, daidzin, genistein, and genistin; these isoflavonoids were detected only in the roots of intact plants.
Furthermore, the isoflavonoid contents of the cell suspension cultures were higher than those of intact plants. Thus, cell
suspension culture of both varieties of P. candollei may be an effective tool for isoflavonoid production. 相似文献
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Sirintra Korsangruang Noppamas Soonthornchareonnon Yupyn Chintapakorn Promchit Saralamp Sompop Prathanturarug 《Plant Cell, Tissue and Organ Culture》2010,103(3):333-342
This study demonstrates the effects of various concentrations of abiotic and biotic elicitors on the cell growth and isoflavonoid
accumulation of P. candollei var. mirifica (PM) and P. candollei var. candollei (PC) cell suspension cultures. The two plant varieties exhibited different growth responses and varied isoflavonoid accumulation
after the addition of elicitors. Copper sulfate, methyl jasmonate (MeJA), and yeast extract did not significantly affect the
growth of either plant variety, whereas oligosaccharide and the biotic elicitors used in this study [i.e., 50 mg l−1 chitosan and all concentrations of laminarin (LAM)] suppressed the growth of PM. The addition of MeJA to the medium principally
induced an effect on the isoflavonoid content in both PM and PC, with 2.0 μM MeJA inducing the highest isoflavonoid content,
as indicated by the induction index—4.41 in PM and 9.62 in PC cells on the 12th and ninth day of culture, respectively. A
maximum total isoflavonoid content of 40.49 mg g−1 dry weight was achieved in PM 21 days after elicitation with 2.0 μM MeJA. LAM elicited the PM cell suspension culture to
produce puerarin, which was not found in the unelicited culture. The results of this study provide information that will be
useful for enhancing the accumulation of isoflavonoids in P. candollei cell suspension cultures. 相似文献
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