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1.
T. A. Timofeeva 《Systematic parasitology》1995,32(1):71-77
Two new capsalid species, Pseudallobenedenia arabica n. sp. from the gills of Pristipomoides filamentosus (Lutjanidae) from the Arabian Sea and Lagenivaginopseudobenedenia tinrowi n. sp. from the gills of Etelis carbunculus (Lutjanidae) from the eastern Pacific Ocean are described and illustrated. P. arabica differs from the most similar species, P. opakapaka Yamaguti, 1966, in the greater width of the body, its larger haptor and anterior adhesive discs, the form and sizes of the testes and ovary, and differences in host and locality. L. tinrowi differs from its congener, L. etelis Yamaguti, 1966, in the larger size of the body and its organs, the absence of eye-spots and Goto's glands, and differences in the arrangement of the genital organs. All of the species in these two genera differ from other capsalids by the presence of a voluminous uterus and an extremely long penis forming a loop. All species in these two genera are parasites of fishes of the family Lutjanidae and are reported from Indo-Pacific subtropical areas. 相似文献
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A V Bursian V O Voskresenski? O P Timofeeva 《Zhurnal evoliutsionno? biokhimii i fiziologii》1987,23(1):115-120
Studies have been made on the organization of the spontaneous motor activity during first 2 weeks of postnatal life of rats Rattus norvegicus (var albin.) by means of prolonged EMG recording from the gastrocnemius muscle and by computer analysis. Complexes of prolonged activity with a period of about 1 min, intracomplex periodicity of short pulses with a frequency 1/sec and periodicity of separate short bursts arranged into series with intervals between them of approximately 8-12 sec were observed. It was found that the main rhythm of these forms of excitation remains relatively unchanged during postnatal ontogenesis. The number of periods filled by series of short bursts decreases in postnatal development of animals, whereas the number of periods of a complete rest lasting for 1 1/2 and more minutes increases. These findings were compared with the development of sleep--wakefulness cycle in early postnatal ontogenesis. 相似文献
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B I Kotliar N O Timofeeva 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》1983,33(6):1059-1066
Tonic background activity of 266 neurones in the hippocampus and different neocortical areas was studied in freely moving rabbits in the process of defensive and food instrumental conditioned performance and during switching-over of instrumental and classical food and defensive reflexes. Associations of CS and reinforcement evoke background activity changes in most of recorded cortical neurones preceding the development of other conditioned manifestations. Conditioned reflex was performed only after reaching the background firing rate of almost every examined neurone optimal for its realization. The performance of different conditioned reflexes was associated with different background activity levels of cortical neurones. The above mentioned data form the experimental basis for the identification of the tonic component in conditioned process which reflects tonic character of temporary connection formation and function. 相似文献
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The acrosomal status of wallaby spermatozoa was evaluated by light and electron microscopy after incubation in 1–100 μM lysophosphatidylcholine (LPC) for up to 120 min. Treatment with 1 and 10 μM LPC for 120 min did not lead to acrosomal loss, or detectable alteration to the acrosome, as detected by Bryan's staining and light microscopy. Incubation with 25 μM LPC had little effect on acrosomal loss, however statistically significant changes (P < 0.05) in the acrosomal matrix (altered) were detected after 10-min incubation by light microscopy. Around 50% of acrosomes were altered after 20-min incubation in 50 μM LPC (P < 0.001), and 40% of spermatozoa had lost their acrosome after 60-min incubation (P < 0.001). Treatment with 75 and 100 μM LPC led to rapid acrosomal loss from around 50% of spermatozoa within 10 min (P < 0.001), and by 60 min acrosomal loss was 70–80%. LPC, like the diacylglycerol DiC8 (1,2-di-octanoyl-sn-glycerol), is thus an effective agent to induce loss of the relatively stable wallaby sperm acrosome, and it also induces changes within the acrosomal matrix. Ultrastructure of the LPC-treated spermatozoa revealed that the plasma membrane and the acrosomal membranes were disrupted in a manner similar to that seen after detergent treatment (Triton X-100). There was no evidence of point fusion between the plasma membrane overlying the acrosome and the outer acrosomal membrane. The plasma membrane was the first structure to disappear from the spermatozoa. The acrosomal membranes and matrix showed increasing disruption with time and LPC concentration. Wallaby spermatozoa incubated with LPC at concentrations that induced significant acrosomal loss also underwent a rapid decline in motility that suggested that acrosomal loss may be due to cell damage, rather than a physiological AR. This study concluded that LPC-induced acrosomal loss from tammar wallaby spermatozoa is due to its action as a natural detergent and not as a phosphoinositide pathway intermediate. The study further demonstrates the unusual stability of the marsupial acrosomal membranes. © 1993 Wiley-Liss, Inc. 相似文献