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排序方式: 共有822条查询结果,搜索用时 15 毫秒
1.
Cloning and characterization of the L-rhamnose regulon in Salmonella typhimurium LT2. 总被引:3,自引:0,他引:3
A Salmonella typhimurium LT2 cosmid library was constructed, and a 46-kb recombinant plasmid was isolated that could complement an Escherichia coli rha mutant. Subsequent subcloning generated a 13.6-kb ClaI restriction fragment that contained a functional regulatory element. By complementation analyses using different subclones, the approximate physical locations of rhaT, rhaC1, rhaC2, rhaB, rhaA, and rhaD were determined. The nucleotide sequence spanning the rhaB and rhaC2 genes was determined. 相似文献
2.
Aileen F. G. Bongat Rina Saksena Roberto Adamo Yukari Fujimoto Zenyu Shiokawa Dwight C. Peterson Koichi Fukase Willie F. Vann Pavol Kováč 《Glycoconjugate journal》2010,27(1):69-77
Using recombinant tetanus toxin HC fragment (rTT-HC) as carrier, we prepared multimeric bivalent immunogens featuring the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Ogawa, in combination with either the synthetic hexasaccharide fragment of O-PS of Vibrio cholerae O:1, serotype Inaba, or a synthetic disaccharide tetrapeptide peptidoglycan fragment as adjuvant. The conjugation reaction
was effected by squaric acid chemistry and monitored in virtually real time by SELDI-TOF MS. In this way, we could prepare
well-defined immunogens with predictable carbohydrate–carrier ratio, whose molecular mass and the amount of each saccharide attached could be independently determined. The ability to prepare such neoglycoconjugates opens unprecedented possibilities
for preparation of conjugate vaccines for bacterial diseases from synthetic carbohydrates. 相似文献
3.
4.
M Dasso H Nishitani S Kornbluth T Nishimoto J W Newport 《Molecular and cellular biology》1992,12(8):3337-3345
Temperature-sensitive mutants in the RCC1 gene of BHK cells fail to maintain a correct temporal order of the cell cycle and will prematurely condense their chromosomes and enter mitosis at the restrictive temperature without having completed S phase. We have used Xenopus egg extracts to investigate the role that RCC1 plays in interphase nuclear functions and how this role might contribute to the known phenotype of temperature-sensitive RCC1 mutants. By immunodepleting RCC1 protein from egg extracts, we find that it is required for neither chromatin decondensation nor nuclear formation but that it is absolutely required for the replication of added sperm chromatin DNA. Our results further suggest that RCC1 does not participate enzymatically in replication but may be part of a structural complex which is required for the formation or maintenance of the replication machinery. By disrupting the replication complex, the loss of RCC1 might lead directly to disruption of the regulatory system which prevents the initiation of mitosis before the completion of DNA replication. 相似文献
5.
6.
M Ohta K Ohta H Hara N Itoh Y Itagaki H Nishitani K Hayashi 《Biochemistry international》1991,25(6):1087-1093
We synthesized a peptide designated R8 (amino acid residues 1157-1201) based on the primary structure presumed from the nucleotide sequence of the cDNA clone from the gene for Duchenne muscular dystrophy. Antibody to the synthetic R8 generated by immunization of rabbits was tested on human and mouse skeletal muscle by Western blotting analysis. The antibody reacted with a component of the 400K dystrophin of normal human and mouse skeletal muscles, but not with components of the muscles of Duchenne muscular dystrophy patients and mdx mice. Thus we established that this peptide sequence is in fact missing in the protein product 'dystrophin' encoded by the DMD gene. The antibody may prove useful for the diagnosis of the Duchenne types of muscular dystrophy. 相似文献
7.
8.
In order to study the IAA-induced modifications of the cellwall of azuki bean (Vigna angularis Ohwi et Ohashi cv. Takara)epicotyl segments, the xyloglucans were subfractionated intotwo components, i.e., 4K-U and 24K xyloglucans, which were obtainedby extraction with 4% KOH solution containing 8 M urea and 24%KOH solution, respectively. The weight-average molecular weightsof 4K-U and 24K xyloglucans were estimated to be 40 x 104 and106 x 104, respectively. Complete acid hydrolysis of 4K-U and24K xyloglucans gave glucose, xylose, galactose and fucose inmole % 48.3 : 33.5 : 13.8 : 4.4 and 45.3 : 30.9 : 19.6 : 4.3,respectively. Treatment of epicotyl segments with IAA (0.1 mM) caused a decreasein the amount of 24K xyloglucans and an increase in 4K-U xyloglucans,whereas the total amount of the two xyloglucans remained constant.Furthermore, IAA treatment caused a decrease in the molecularweight of 24K xyloglucans from 106 x 104 to 78 x 104 withoutcausing changes in their sugar compositions. With 4K-U xyloglucans,IAA caused an increase in the mole % of xylose and a decreasein the mole % of galactose and fucose.
1 This paper is dedicated to the late Professor Joji Ashida. (Received November 26, 1982; Accepted February 7, 1983) 相似文献
9.
Hideo Nishitani Masumi Hidaka Takashi Horiuchi 《Molecular genetics and genomics : MGG》1993,240(3):307-314
To clone new replication origin(s) activated under RNase H-defective (rnh ?) conditions in Escherichia coli cells, whole chromosomal DNA digested with EcoRI was to with a Kmr DNA fragment and transformed into an rnh? derivative host. From the Kmr transformants, we obtained eight kinds of plasmid-like DNA, each of which contained a specific DNA fragment, termed “Hot”, derived from the E. coli genome. Seven of the Hot DNAs (HotA-G) mapped to various sites within a narrow DNA replication termination region (about 280 kb), without any particular selection. Because Hot DNA could not be transformed into a mutant strain in which the corresponding Hot region had been deleted from the chromosome, the Hot DNA, though obtained as covalently closed circular (ccc) DNA, must have arisen by excision from the host chromosome into which it had initially integrated, rather than by autonomous replication of the transformed species. While Hot DNA does not have a weak replication origin it does have a strong recombinational hotspot active in the absence of RNase H. This notion is supported by the finding that Chi activity was present on all Hot DNAs tested and no Hot-positive clone without Chi activity was obtained, with the exception of a DNA clone carrying the dif site. 相似文献
10.
Immunocytochemical localization of thrombomodulin in the aqueous humor passage of the rat eye 总被引:1,自引:1,他引:0
T. Daimon Mutsuyoshi Kazama Yukari Miyajima Masahiko Nakano 《Histochemistry and cell biology》1997,108(2):121-131
This report describes the distribution and localization of thrombomodulin (TM) in the rat eye by light and electron microscopic
immunocytochemistry. In addition to the endothelium of the entire vasculature, TM was found on the non-vascular structures
lining the cavities of the posterior and anterior chambers and the limbus. TM was localized on the basal, lateral, and apical
plasma membranes of the inner and outer ciliary epithelium, and the posterior iris epithelium in which there was no polarized
expression of TM. In the anterior chamber, TM was localized on the luminal surface of the corneal endothelium, but was negative
on the anterior border layer of the iris, which is composed of a discontinuous layer of fibroblasts and collagen fibers. Thus,
TM was present at sites of cell-to-cell contact. TM was also present on the endothelia of the trabecular meshwork and the
Schlemm’s canal in the limbus. TM was localized not only on the luminal plasma membrane, but also on the cytoplasmic giant
vacuoles in the endothelial cells of the Schlemm’s canal. These findings extend the importance of anticoagulant mechanisms
to the systems of secretion, circulation, and drainage of the aqueous humor.
Accepted: 18 March 1997 相似文献