克山病是一种“心肌线粒体病(Mitochondrial Cardiomyopathy)”

亚急克病人心肌线粒体内膜电子传递链的琥珀酸氧化酶系,琥珀酸脱氢酶和细胞色素氧化酶活性明显低于对照。H~ -ATP酶的活性及其对寡霉素的敏感性都明显下降。ATP能量化后线粒体膜电位的变化也比对照明显降低。膜脂流动性低于对照。亚急克病人心肌线粒体内观察到较多的电子致密无定形物质,经电镜X射线微区等方法分析,认为这些物质不是Ca_3(PO_4)_2,而可能是一种蛋白质凝聚物。此外,心肌线粒体的硒含量远低于对照,而Ca含量明显高于对照。上述结果都反映亚急克病人心肌线粒体明显损伤。根据克山病患者心肌细胞线粒体结构与功能方面呈现的如此广泛与明显的异常,可将克山病称为“心肌线粒体病(Mitochondrial Cardiomyopathy)”。     

阿胶冲剂与阿胶的化学成分对比分析研究

阿胶在我国有着悠久的历史,是常用滋补中药,具有许多特殊的功效,国内外享有盛誉。据《本草纲目》及《本草纲目拾遗》等介绍,阿胶在明代以前系用(沙牛)牛,水牛,驴皮或猪、马、驼皮等杂皮熬制而成。自清代始一律采用黑驴皮熬制。以往服用阿胶必须将阿胶先用水浸泡后,然后加热溶解后才能服用.为了克服这一缺点,山东化工学院研制了一种用开水冲服的阿胶新制剂——阿胶冲剂.为了弄清阿胶经物理方法处理后的化学组成成份与原阿谱的化学组成成分的不同变化。我们对其所含微量元素、氨基酸进行了对比分析.另外,本文首次报道了阿胶及阿胶冲剂的红外光谱分析结果.     

Three-dimensional models of non-NMDA glutamate receptors.

Structural models have been produced for three types of non-NMDA inotropic glutamate receptors: an AMPA receptor, GluR1, a kainate receptor, GluR6; and a low-molecular-weight kainate receptor from goldfish, GFKAR alpha. Modeling was restricted to the domains of the proteins that bind the neurotransmitter glutamate and that form the ion channel. Model building combined homology modeling, distance geometry, molecular mechanics, interactive modeling, and known constraints. The models indicate new potential interactions in the extracellular domain between protein and agonists, and suggest that the transition from the "closed" to the "open" state involves the movement of a conserved positive residue away from, and two conserved negative residues into, the extracellular entrance to the pore upon binding. As a first approximation, the ion channel domain was modeled with a structure comprising a central antiparallel beta-barrel that partially crosses the membrane, and against which alpha-helices from each subunit are packed; a third alpha-helix packs against these two helices in each subunit. Much, but not all, of the available data were consistent with this structure. Modifying the beta-barrel to a loop-like topology produced a model consistent with available data.     

细胞寒害的热力学熵理论(Ⅱ)──超熵产生作为寒害稳定性判据的理论研究

本文从物质和能量交换的角度,运用非平衡态热力学超熵产生理论,分析了寒害定态的稳定性,并建立了超熵产生判据．理论分析所得的结论与实验结果基本相符．     

长窦副新蚤雄蚤的形态记述(蚤目:多毛蚤科)

本文首次记述了采自青海玉树的长窦副新蚤Paraneopsylla longisinuata Liu, Tsai & Wu,1974的雄蚤。模式标本保存于著者所在的单位。     

壳聚糖酶结构与功能的研究进展

壳聚糖酶是一类对壳聚糖具有较高催化活性而几乎不水解几丁质的糖苷水解酶,其可将高分子量的壳聚糖转化为低分子量的功能性壳寡糖。近年来,对壳聚糖酶的相关研究取得了显著进展,因此,本文对其生化性质、晶体结构、催化机制和蛋白质工程改造进行总结和探讨,并对酶法制备壳寡糖纯品进行展望,这将加深研究者对壳聚糖酶作用机制的认识,推动壳聚糖酶的工业应用。     

棉铃虫的呼吸代谢

棉铃虫的耗氧量随着幼虫的生长发育而增加。当发育状态基本相同时,在同一龄期内,幼虫耗氧量与虫体鲜重呈直线相关;在不同龄期之间,耗氧量则与虫体鲜重的0.97次方成正比。幼虫的代谢速率随着龄期的增加而稳定地降低。在20—35℃范围内,温度每升高10℃,幼虫的代谢速率约增加一倍。蛹期的代谢速率呈典型的“U”形曲线变化。从卵期及幼虫期到蛹期的呼吸商变动在0.75—0.96之间。