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Because resources for long-term care services are limited, timely and appropriate referral for rehabilitation services is critical for optimizing clients’ functions and successfully integrating them into the community. We investigated which client characteristics are most relevant in predicting Taiwan’s community-based occupational therapy (OT) service referral based on experts’ beliefs. Data were collected in face-to-face interviews using the Multidimensional Assessment Instrument (MDAI). Community-dwelling participants (n = 221) ≥ 18 years old who reported disabilities in the previous National Survey of Long-term Care Needs in Taiwan were enrolled. The standard for referral was the judgment and agreement of two experienced occupational therapists who reviewed the results of the MDAI. Logistic regressions and Generalized Additive Models were used for analysis. Two predictive models were proposed, one using basic activities of daily living (BADLs) and one using instrumental ADLs (IADLs). Dementia, psychiatric disorders, cognitive impairment, joint range-of-motion limitations, fear of falling, behavioral or emotional problems, expressive deficits (in the BADL-based model), and limitations in IADLs or BADLs were significantly correlated with the need for referral. Both models showed high area under the curve (AUC) values on receiver operating curve testing (AUC = 0.977 and 0.972, respectively). The probability of being referred for community OT services was calculated using the referral algorithm. The referral protocol facilitated communication between healthcare professionals to make appropriate decisions for OT referrals. The methods and findings should be useful for developing referral protocols for other long-term care services. 相似文献
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Ya-Chung Tian Yi-Jung Li Hua-Chien Chen Hsin-Hsu Wu Cheng-Hao Weng Yung-Chang Chen Cheng-Chia Lee Ming-Yang Chang Hsiang-Hao Hsu Tzung-Hai Yen Cheng-Chieh Hung Chih-Wei Yang 《Biochemical and biophysical research communications》2014
Polyomavirus BK (BKV) infection is an important cause of renal allograft failure. Viral microRNAs are known to play a crucial role in viral replication. This study investigated the expression of BKV-encoded microRNAs (miR-B1) in patients with polyomavirus-associated nephropathy (PVAN) and their role in viral replication. Following BKV infection in renal proximal tubular cells, the 3p and 5p miR-B1 levels were significantly increased. Cells transfected with the vector containing the miR-B1 precursor (the miR-B1 vector) showed a significant increase in expression of 3p and 5p miR-B1 and decrease in luciferase activity of a reporter containing the 3p and 5p miR-B1 binding sites, compared to cells transfected with the miR-B1-mutated vector. Transfection of the miR-B1 expression vector or the 3p and 5p miR-B1 oligonucleotides inhibited expression of TAg. TAg-enhanced promoter activity and BKV replication were inhibited by miR-B1. In contrast, inhibition of miR-B1 expression by addition of miR-B1 antagomirs or silencing of Dicer upregulated the expression of TAg and VP1 proteins in BKV-infected cells. Importantly, patients with PVAN had significantly higher levels of 3p and 5p miR-B1 compared to renal transplant patients without PVAN. In conclusion, we demonstrated that (1) miR-B1 expression was upregulated during BKV infection and (2) miR-B1 suppressed TAg-mediated autoregulation of BKV replication. Use of miR-B1 can be evaluated as a potential treatment strategy against BKV infection. 相似文献
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Kung YJ Lin SS Huang YL Chen TC Harish SS Chua NH Yeh SD 《Molecular Plant Pathology》2012,13(3):303-317
MicroRNAs (miRNAs) regulate the abundance of target mRNAs by guiding cleavage at sequence complementary regions. In this study, artificial miRNAs (amiRNAs) targeting conserved motifs of the L (replicase) gene of Watermelon silver mottle virus (WSMoV) were constructed using Arabidopsis pre-miRNA159a as the backbone. The constructs included six single amiRNAs targeting motifs A, B1, B2, C, D of E, and two triple amiRNAs targeting motifs AB1E or B2DC. Processing of pre-amiRNAs was confirmed by agro-infiltration, and transgenic Nicotiana benthamiana plants expressing each amiRNA were generated. Single amiRNA transgenic lines expressing amiR-LB2 or amiR-LD showed resistance to WSMoV by delaying symptom development. Triple amiRNA lines expressing amiR-LB2, amiR-LD and amiR-LC provided complete resistance against WSMoV, with no indication of infection 28 days after inoculation. Resistance levels were positively correlated with amiRNA expression levels in these single and triple amiRNA lines. The triple amiR-LAB1E line did not provide resistance to WSMoV. Similarly, the poorly expressed amiR-LC and amiR-LE lines did not provide resistance to WSMoV. The amiR-LA- and amiR-LB1-expressing lines were susceptible to WSMoV, and their additional susceptibility to the heterologous Turnip mosaic virus harbouring individual target sequences indicated that these two amiRNAs have no effect in vivo. Transgenic lines expressing amiR-LB2 exhibited delayed symptoms after challenge with Peanut bud necrosis virus having a single mismatch in the target site. Overall, our results indicate that two amiRNAs, amiR-LB2 and amiR-LD, of the six designed amiRNAs confer moderate resistance against WSMoV, and the triple construct including the two amiRNAs provides complete resistance. 相似文献
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Wen-Hsiung Ko Yi-Jung Tsou Yu-Ming Ju Huei-Mei Hsieh Pao-Jen Ann 《Mycopathologia》2010,169(2):125-131
Four fungal isolates that were able to use vegetable tissues for multiplication in soil were isolated and identified as Pseudallescheria boydii based on morphological characteristics and ITS sequence similarity. When grown in broth prepared from the same vegetable
tissues used in soil amendment, all these isolates of P. boydii produced a substance capable of reducing the disease incidence of black leaf spot of spoon cabbage caused by Alternaria brassicicola and inhibiting the germination of A. brassicicola conidia. The substance, which was fungistatic, was very stable under high temperature and high or low pH value. It was soluble
in polar solvents and insoluble in non-polar solvents. Molecular weight estimation and ion exchange ability tests suggest
that the fungistatic compound has a molecular weight between 500 and 1,000 and has no charge on its molecule. Results from
this study suggest the possession of a strong competitive saprophytic ability by P. boydii, which in turn may explain the widespread occurrence of this human pathogen in soil. Production of a fungistatic substance
when P. boydii was grown in broth prepared from vegetable tissues suggests the importance of antibiotic production in its competitive saprophytic
colonization of organic matters in soil. 相似文献
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Transgene-specific and event-specific molecular markers for characterization of transgenic papaya lines resistant to Papaya ringspot virus 总被引:1,自引:0,他引:1
Ming-Jen Fan Shu Chen Yi-Jung Kung Ying-Huey Cheng Huey-Jiunn Bau Tien-Tsai Su Shyi-Dong Yeh 《Transgenic research》2009,18(6):971-986
The commercially valuable transgenic papaya lines carrying the coat protein (CP) gene of Papaya ringspot virus (PRSV) and conferring virus resistance have been developed in Hawaii and Taiwan in the past decade. Prompt and sensitive
protocols for transgene-specific and event-specific detections are essential for traceability of these lines to fulfill regulatory
requirement in EU and some Asian countries. Here, based on polymerase chain reaction (PCR) approaches, we demonstrated different
detection protocols for characterization of PRSV CP-transgenic papaya lines. Transgene-specific products were amplified using
different specific primer pairs targeting the sequences of the promoter, the terminator, the selection marker, and the transgene,
and the region across the promoter and transgene. Moreover, after cloning and sequencing the DNA fragments amplified by adaptor
ligation-PCR, the junctions between plant genomic DNA and the T-DNA insert were elucidated. The event-specific method targeting
the flanking sequences and the transgene was developed for identification of a specific transgenic line. The PCR patterns
using primers designed from the left or the right flanking DNA sequence of the transgene insert in three selected transgenic
papaya lines were specific and reproducible. Our results also verified that PRSV CP transgene is integrated into transgenic
papaya genome in different loci. The copy number of inserted T-DNA was further confirmed by real-time PCR. The event-specific
molecular markers developed in this investigation are crucial for regulatory requirement in some countries and intellectual
protection. Also, these markers are helpful for prompt screening of a homozygote-transgenic progeny in the breeding program. 相似文献
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Chen Boryuan Yeh Han-Yang Huang Chih-Hsien Lung Wei Qing Chloe Chen Yi-Jung Lee Po-Tsang Nan Fan-Hua Lee Meng-Chou 《Journal of applied phycology》2022,34(3):1201-1213
Journal of Applied Phycology - The cryptophyte alga Proteomonas sulcata is rich in unsaturated fatty acids, phycoerythrin, and other biologically active substances and is suitable for aquatic feed,... 相似文献
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Environmental Biology of Fishes - The Northwest Pacific, including Korea, is an interesting area for population genetic studies because of the dramatic Pleistocene sea-level change. The ice goby... 相似文献
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