The Phospholipid Composition of Nuclear Subfractions from Germinating Wheat Embryos

The content of phospholipids in chromatin, nuclear matrix, and nuclear membrane from wheat (Triticum aestivum L.) embryos was studied. Subfractions of intact nuclei from dry embryos were shown to differ in the content and composition of particular phospholipids. Embryo germination resulted in the redistribution of phospholipid between nuclear subfractions. A functional role of structural changes in the nuclear membrane due to this phospholipid redistribution is discussed. It is supposed that these rearrangements change nuclear membrane permeability and its surface charge.     

Changes in the composition of phospholipids in nuclear subfractions of wheat seedlings treated with gibberellin

Macromolecule transport between the cytoplasm and nucleus occurs through the nuclear pore complexes with the help of soluble transport factors. The receptors of GA are not yet identified, and the molecular mechanism of plant response to GA is not known so far. We compared the content of phospholipids in the soluble nuclear fraction and nuclear membrane of wheat seedlings on the third and fourth days of germination after treatment with GA. It was shown that GA induced differently directed changes in the composition of phospholipids in nuclear subfractions tested. Changes in the composition of the nuclear membrane are supposed to be involved in the control of gene expression by GA.