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Hippophae rhamnoides ssp. sinensis occurs mainly in the arid regions of northwest China. The wood stands of this subspecies play an important role in maintaining
the local ecosystems in these regions. In addition, the genetic characteristics are essential to understand the historical
range changes of this subspecies and its morphological differentiation with other subspecies. In this study, we developed
nine microsatellite loci for this subspecies for the first time. We used the combining biotin capture method to enrich AG/CT/AC/GT/CG/GTG/CCA
microsatellites. Twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic
through screening 12 distantly distributed individuals. The number of alleles per locus ranged from three to twelve and expected
heterozygosity from 0.2659 to 0.4767, respectively. We further performed cross-priming tests in another subspecies and two
congeneric species. These firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies
and its morphological differentiation from the other subspecies. 相似文献
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