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1.
An investigation of the hydrolysis of various substrates (l-Trp-OMe, l-Phe-OMe, l-Leu-OMe and l-Val-OMe) by pronase has shown that the highest activity is displayed with l-Leu-OMe substrate. Addition of Ca2+ ions significantly enhances the rate of hydrolysis of l-Leu-OMe without affecting the hydrolysis of d-Leu-OMe. Thus, the enantioselectivity of the process is improved. 相似文献
2.
A reductive LiBH4-ButOH cleavage of N-glycosylamide carbohydrate-peptide bond allowed splitting off of oligosaccharide chains of the fucolectin, the bark agglutinin from the shrub golden rain Laburnum anagyroides (LABA). Four N-glycans were isolated by HPLC, and their structures were elucidated by monosaccharide analysis and 1H NMR (500 MHz) spectroscopy: Man2Fuc1Xyl1GlcNAc2 (M2FX), Man3Xyl1GlcNAc2 (M3X), Man3Fuc1Xyl1GlcNAc2 (M3FX), and Man3Xyl1Fuc1GlcNAc3 (NM3FX). All the N-glycans contain D-xylose and three of them, L-fucose; they were found to be in a 1:8:3:1 ratio. 相似文献
3.
Yamskov I. A. Vinogradov A. A. Danilenko A. N. Maslova L. A. Rybakova E. Yu. Yamskova V. P. 《Applied Biochemistry and Microbiology》2001,37(1):29-35
The amino acid composition, structure, and physicochemical properties of a low-molecular-weight glycoprotein from cattle blood serum (SGP) were studied. The content of carbohydrates (represented by mannose-rich oligosaccharides) amounted to 45–50 wt %. The value of the specific partial heat of SGP, measured by differential scanning calorimetry (DSC), equaled 1.8 J/(g K), which is characteristic of unfolded proteins. Circular dichroic (CD) spectra of SGP led us to conclude that it is not highly structured and that it occurs in the shape of a statistical globule. The protein was deglycated using anhydrous trifluoromethane sulfonate (TFMS), after which its amino acid composition and the sequence of a fragment were determined. The results indicate that SGP is a protein not studied previously. 相似文献
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5.
E. V. Sidorskii A. P. Il’ina M. S. Krasnova V. P. Yamskova A. K. Buryak I. A. Yamskov 《Applied Biochemistry and Microbiology》2018,54(1):83-88
The peptide–protein complex from bovine sclera was studied. It is shown that it contained a protein with a molecular weight of 66387 Da with the partial N-terminal amino acid sequence DTHKSEIAHRFKDLG-, which is homologous to the mature molecule of bovine serum albumin, and polypeptides with molecular weights of 1300–5080 Da. With a model of the organotypic cultivation of posterior eye tissues of the newt Pleurodeles waltl in vitro, it was shown that the effect of this peptide–protein complex in low doses increased the viability of scleral fibroblasts. 相似文献
6.
A. P. Il’ina O. G. Kulikova D. I. Maltsev M. S. Krasnov E. Yu. Rybakova V. S. Skripnikova E. S. Kuznetsova A. K. Buryak V. P. Yamskova I. A. Yamskov 《Applied Biochemistry and Microbiology》2011,47(2):118-122
We performed the matrix-assisted laser desorption/ionisation, time-of-flight mass spectrometry (MALDI-TOF) analysis of the peptides entering into the composition of not yet explored bioregulators derived from the extracellular
matrix of the tissues of the various organs of the mammals, and also plants and fungi. The study included 15 different mammalian
tissues, 13 species of plants, and 2 species of fungi. Exploring the bioregulators derived from eye tissues, we demonstrated
that their composition includes peptide components with the same values of the molecular weight. The composition of the bioregulators
derived from the tissues of various organs of mammals or different species of plants and fungi includes the peptides with
different values of molecular weight. Obtained data indicate the growing evidence of the assumptions about the major function
of the bioregulators of this group—their involvement in the regulation of tissue-organ homeostasis in the biological systems. 相似文献
7.
M. S. Krasnov E. N. Grigoryan V. P. Yamskova A. N. Danilenko I. A. Yamskov 《Russian Journal of Developmental Biology》2000,31(5):309-313
Two groups of proteins were isolated from the retina and pigment epithelium of eight-day-old chick embryos. Experiments with
suspension cultures of retinal cells demonstrated that only the retinal extracts and the fraction of its acidic proteins can
stimulate cell aggregation in vitro. Analysis by the method of high-performance liquid chromatography showed that fractions
of acidic and basic retinal proteins, which markedly differ in their electric charge and biological activity, have similar
composition. To study the effect of these proteins on the morphological and functional state of pigment epitheliumin vitro, a new experimental model is proposed, with the posterior segment of the newt (Pleurodeles waltl) eye used as a test tissue. The fraction of basic proteins isolated from the chick embryonic pigment epithelium stabilized
cell differentiation in the newt pigment epithelium. The analyzed proteins proved to be biologically active at extremely low
doses, corresponding to 10−12 M solutions. 相似文献
8.
V. P. Yamskova E. Yu. Rybakova A. A. Vinogradov V. V. Vecherkin I. A. Yamskov 《Applied Biochemistry and Microbiology》2004,40(4):345-350
A protein with a molecular weight of 70 kDa was isolated from bovine blood serum and purified to a homogenous state. This protein reversibly inhibited the adhesive serum glycoprotein with a molecular weight of 12 kDa, which displayed biological activity at ultralow doses. Amino acid analysis showed that the protein inactivator belongs to the group of prealbumins from vertebrate blood serum. The secondary structure of its molecule was characterized by a considerable number of -helices. The conditions for inactivation of serum glycoprotein were studied. The interaction between the serum glycoprotein and the protein inactivator occurred over a long period of time (1 day). It should be emphasized that the presence of calcium ions was a necessary condition for the inactivation of the serum glycoprotein. The data suggest that inactivation of serum glycoprotein results from the formation of a molecular complex consisting of the protein inactivator and the glycoprotein, which is related to the carbon–protein interaction. 相似文献
9.
Piskarev V. E. Lutsik-Kordovskii M. D. Piskareva E. L. Yamskov I. A. 《Applied Biochemistry and Microbiology》2003,39(5):512-518
A comparative study of fine carbohydrate specificity of the lectin from the bark of laburnum Laburnum anagyroides (LABA) and the fucolectin from asparagus pea Tetragonolobus purpureus (TPA) was performed using inhibition of agglutination of the complex formed by H-active neoglycoprotein and nanoparticles of colloidal gold. Both lectins bound most strongly the H type 2 oligosaccharides comprising O-glycans; however, LABA was almost unable to discriminate between them. LABA bound more weakly the H type 6 trisaccharide (Fuc1-2Gal1-4Glc) and difucosyllactose (Fuc1-2Gal1-4[Fuc1-3]Glc), a glucoanalogue of the Ley antigen, and, even more weakly, the Lea pentasaccharide lacto-N-fucopentaose II (Gall-3[Fucl-4]GlcNAcl-3Gall-4Glc). However, LABA did not bind the antigens Leb, Lec, and Led, very poorly interacted with the terminal Lex, and somewhat more strongly bound the internal Lex. The lectin also had a hydrophobic binding site. Both lectins exhibited a cluster effect with polymeric ligands (neoglycoproteins). 相似文献
10.
Samojlova NA Krayukhina MA Yamskov IA 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,800(1-2):263-269
The principle of affinity chromatography was used for preparation of thromboresistant bilayer coatings. The outer biospecific layer containing epsilon-aminocaproic acid residues (from 2.2 up to 5.5 nmol/cm2) was synthesized using a copolymer of maleic anhydride with N-vinylpyrrolidone and L-lysine dihydrochloride or N-epsilon-tert-BOC-L-lysine. This surface can selectively adsorb plasminogen (fibrinolytic zymogen) from blood. The biospecific layer (from 2.0 up to 3.6 microg/cm2) was applied for covering chitosan (native or modified) or albumin interlayer. Such bilayer coatings (BCs) were stable and represented the insoluble polyelectrolyte complexes. BCs were proposed for bilayer modification of synthetic vascular grafts, polyethylene, and other materials contacting with blood. This technique allowed us to significantly reduce thrombogenic properties of polyethylene surfaces. 相似文献