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1.
EMI TSUCHIDA AKIKO FUJIWARA YUKIO FUJINO TAKARA YONAGA IKUO YASUMASU 《Development, growth & differentiation》1985,27(1):63-72
During initial several minutes after fertilization, sea urchin eggs exhibited high rate of respiration which was only slightly inhibited by cyanide. This cyanide-insensitive respiration was inhibited by calcium antagonists, diltiazem and verapamil, and calmodulin antagonists, N-(6-aminohexyl)-5-chloro-1-naphthalensulfonamide hydrochloride (W-7), N-(6-aminohexyl)-1-naphthalenesulfonamide hydrochloride (W-5) and chlorpromazine, which were added within 1 min after insemination. The inhibitory effect of W-7 on cyanide-insensitive respiration was higher than that of W-5. Cyanide-sensitive respiration of fertilized eggs observed after this initial period was not inhibited by these compounds. Ca2+ influx in eggs just after fertilization was inhibited by calcium antagonists but was rather enhanced by calmodulin antagonists. Fertilization-induced stimulation of cyanide-insensitive respiration probably results from calmodulin-dependent reactions which are activated by Ca2+ influx. 相似文献
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N. YASUDA S. NAGAI S. YAMAGUCHI C. L. LIAN M. HAMAGUCHI 《Molecular ecology resources》2007,7(1):43-45
We isolated nine polymorphic microsatellites from the Manila clam Ruditapes philippinarum. These loci provide one class of highly variable genetic marker as the number of alleles ranged from six to 22, and the observed and expected heterozygosity ranged from 0.136 to 0.909 and from 0.553 to 0.954, respectively. We consider that these loci are potentially useful for detailing the genetic structure and gene flow among R. philippinarum populations. 相似文献
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S. NAGAI G. NISHITANI S. YAMAGUCHI N. YASUDA C. L. LIAN S. ITAKURA M. YAMAGUCHI 《Molecular ecology resources》2007,7(6):993-995
We isolated 15 polymorphic microsatellites from one of the most noxious red tide‐causing dinoflagellate species, Heterocapsa circularisquama. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from two to six, and the estimate of gene diversity was from 0.205 to 0.684 across the 15 microsatellites. These loci have the potential to reveal genetic structure and gene flow among H. circularisquama populations. 相似文献
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G. NISHITANI S. NAGAI S. SAKAMOTO C. L. LIAN C. K. LEE T. NISHIKAWA S. ITAKURA M. YAMAGUCHI 《Molecular ecology resources》2007,7(5):827-829
We isolated 15 polymorphic microsatellites from Cochlodinium polykrikoides. These loci provide a class of highly variable genetic markers, as the number of alleles ranged from two to 15, and the estimate of gene diversity was from 0.083 to 0.880 across the 15 microsatellites. We consider that these loci have a potential to reveal the genetic structure and gene flow among C. polykrikoides populations. 相似文献
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- It has been established that the absence of isocitric dehydrogenaseactivity in cotyledons of Vigna sesquipedalisin the germinationstage is due to the lack of endogenous TPN. It is unlikely thatthe TCA cycle in the cotyledon is operative.
- The activitiesof enzymes of the glyoxylate cycle, a modifiedTCA cycle, weremeasured through the germination stage. Theiractivities wereconsiderably higher in the cotyledon tissuethan in the hypocotyl.A dominant metabolic pathway of isocitricacid in the cotyledonmay be the glyoxylate cycle.
- DPN kinase which produces TPNfrom DPN, ATP and Mg++ was localizedin the supernatant portionof cell components in the cotyledon.Its activity was low. DPNkinase serves as a control factorfor the cotyledon metabolism.
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A considerable part of the response of the vacuolar potentialof Nitella flexilis to the change of external KCl, NaCl, RbCl,LiCl, or CaCl2 concentration is caused by the response of thecell wall (a cation exchanger) to the external medium. The potentialswere measured on the internodes whose cell sap was exchangedfor simple salt solutions. The potential difference across theplasmalemma which is the internal potential measured againstthe cell wall phase changes largely with the change in concentrationof the external KCl, but also more or less with that of theexternal NaCl, LiCl or RbCl. CaCl2 depolarizes the plasmalemmapotential by about 50 mv when the concentration is increasedfrom 105 M to 103 M, and hyperpolarizes it againby about 40 mv from 103 M to 101 M leaving thelevel of the peak of the action potential almost unchanged. 1This work was supported by Research Grants from the Ministryof Education of Japan 相似文献
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N. YASUDA S. NAGAI M. HAMAGUCHI C. L. LIAN K. NADAOKA 《Molecular ecology resources》2006,6(1):141-143
We isolated nine polymorphic microsatellites from the crown‐of‐thorns starfish, Acanthaster planci. These loci provide one class of highly variable genetic marker as the number of alleles ranged from three to 12 and the observed and expected heterozygosities ranged from 0.130 to 0.783 and from 0.163 to 0.862, respectively. We consider that these loci are potentially useful for detailing the genetic structure and gene flow among A. planci populations. 相似文献