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1.
通过线粒体控制区序列的分析,研究采自中国南海及东海5个群体102尾细鳞鯻的遗传多样性。发现在962 bp序列中有205个变异位点,其中135个为简约信息位点,共定义102个单倍型。中国近海细鳞鯻总体呈现出较高的遗传多样性特征(Hd=1.000,Pi=0.022),其中博鳌最高(Hd=1.000,Pi=0.028),平潭最低(Hd=1.000,Pi=0.014)。不同地理群体间无明显分化,基因交流频繁(Fst=-0.014—0.041,P0.05);中性检验均为显著负值,推测在16.9万年—5.06万年前,即中-晚更新世出现种群扩张。系统邻接树和单倍型网络图均出现3个显著分化的谱系(谱系间Fst=0.508—0.698,P0.001;净遗传距离Da=0.024—0.031),且各谱系中均有不同地理来源的群体。3个谱系间分歧时间大约在1.07百万年—0.24百万年前,推测可能是更新世冰期边缘海的出现导致群体隔离而产生分化。谱系A(Lineage A)包含85.3%的个体,其总体遗传多样性较高(Hd=1.000,Pi=0.012),其中平潭最高(Hd=1.000,Pi=0.014),合浦最低(Hd=1.000,Pi=0.010);群体间Fst在-0.021—0.068之间,P0.005;AMOVA分析显示只有1.97%的变异来自于种群间,表明群体间也无明显分化;中性检验均为显著负值,推测在25.4万年—7.6万年前出现种群扩张。中国近海细鳞鯻主要受到中-晚更新世海侵和海退的影响而出现种群扩张使得谱系间发生二次接触,最终形成具有显著谱系结构但无地理分化的情况。 相似文献
2.
The microstructure of the main longitudinal veins of the dragonfly wing and the aerodynamic behaviors of the wing were investigated in this paper.The microstructure of longitudinal vein presents two circumferential chitin layers and a protein-fiber soft layer.The dragonfly wing is corrugated due to the spatial arrangement of longitudinal veins.It was found that the corrugation angle could significantly influence the lift/drag ratio across a range of attack angles by the wind tunnel experiments.The results of the finite element analysis indicate that the protein soft layer of vein facilitates the change of the corrugation angle by allowing substantial relative twisting deformation between two neighboring veins,which is not possible in veins without a soft sandwich layer. 相似文献
3.
Effects of Dragonfly Wing Structure on the Dynamic Performances 总被引:2,自引:0,他引:2
The configurations of dragonfly wings, including the corrugations of the chordwise cross-section, the microstructure of the longitudinal veins and membrane, were comprehensively investigated using the Environmental Scanning Electron Microscopy (ESEM). Based on the experimental results reported previously, the multi-scale and multi-dimensional models with different structural features of dragonfly wing were created, and the biological dynamic behaviors of wing models were discussed through the Finite Element Method (FEM). The results demonstrate that the effects of different structural features on dynamic behaviors of dragonfly wing such as natural frequency/modal, bending/torsional deformation, reaction force/torque are very significant. The corrugations of dragonfly wing along the chordwise can observably improve the flapping frequency because of the greater structural stiffness of wings. In updated model, the novel sandwich microstructure of the longitudinal veins remarkably improves the torsional deformation of dragonfly wing while it has a little effect on the flapping frequency and bending deformation. These integrated structural features can adjust the deformation of wing oneself, therefore the flow field around the wings can be controlled adaptively. The fact is that the flights of dragonfly wing with sandwich microstructure of longitudinal veins are more efficient and intelligent. 相似文献
4.
Yu Yifei Hou Kun Ji Tong Wang Xishu Liu Yining Zheng Yangyang Xu Jinying Hou Yi Chi Guangfan 《Molecular and cellular biochemistry》2021,476(5):2111-2124
Molecular and Cellular Biochemistry - MicroRNAs (miRNA), endogenous non-coding RNAs approximately 22 nucleotides long, regulate gene expression by mediating translational inhibition or mRNA... 相似文献
5.
Inactivated SARS-CoV vaccine prepared from whole virus induces a high level of neutralizing antibodies in BALB/c mice 总被引:5,自引:0,他引:5
Tang L Zhu Q Qin E Yu M Ding Z Shi H Cheng X Wang C Chang G Zhu Q Fang F Chang H Li S Zhang X Chen X Yu J Wang J Chen Z 《DNA and cell biology》2004,23(6):391-394
We tested the ability of inactivated SARS-CoV vaccine to induce neutralizing antibodies in BALB/c mice. The inactivated vaccine was prepared by SARS-CoV virus propagation in Vero cells, with subsequent beta-propiolactone inactivation and Sepharose 4FF column chromatography purification. One hundred forty BALB/c female mice were divided into seven groups of 20 mice each. Of the seven groups, three groups were inoculated with 0.1, 1, and 3 microg of the vaccine without adjuvant while three other groups were inoculated at the same three dosages of vaccine with aluminum hydroxide as adjuvant, respectively. The remaining group was set up as a blank control. Each mouse was inoculated twice at an interval of 3 weeks. One week after the second immunization, mice sera were collected to detect serum neutralizing antibodies. An assay for determining neutralizing antibody titers was developed. The results can be summarized as follows: (1) higher dosages of vaccine induced higher levels of neutralizing antibody titer; (2) the level of neutralizing antibodies induced by the inoculation with aluminum hydroxide adjuvant was slightly higher than that without adjuvant, but the difference was not statistically significant. 相似文献
6.
Microbial functional diversity covaries with permafrost thaw‐induced environmental heterogeneity in tundra soil
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Mengting M. Yuan Jin Zhang Kai Xue Liyou Wu Ye Deng Jie Deng Lauren Hale Xishu Zhou Zhili He Yunfeng Yang Joy D. Van Nostrand Edward A. G. Schuur Konstantinos T. Konstantinidis Christopher R. Penton James R. Cole James M. Tiedje Yiqi Luo Jizhong Zhou 《Global Change Biology》2018,24(1):297-307
Permafrost soil in high latitude tundra is one of the largest terrestrial carbon (C) stocks and is highly sensitive to climate warming. Understanding microbial responses to warming‐induced environmental changes is critical to evaluating their influences on soil biogeochemical cycles. In this study, a functional gene array (i.e., geochip 4.2) was used to analyze the functional capacities of soil microbial communities collected from a naturally degrading permafrost region in Central Alaska. Varied thaw history was reported to be the main driver of soil and plant differences across a gradient of minimally, moderately, and extensively thawed sites. Compared with the minimally thawed site, the number of detected functional gene probes across the 15–65 cm depth profile at the moderately and extensively thawed sites decreased by 25% and 5%, while the community functional gene β‐diversity increased by 34% and 45%, respectively, revealing decreased functional gene richness but increased community heterogeneity along the thaw progression. Particularly, the moderately thawed site contained microbial communities with the highest abundances of many genes involved in prokaryotic C degradation, ammonification, and nitrification processes, but lower abundances of fungal C decomposition and anaerobic‐related genes. Significant correlations were observed between functional gene abundance and vascular plant primary productivity, suggesting that plant growth and species composition could be co‐evolving traits together with microbial community composition. Altogether, this study reveals the complex responses of microbial functional potentials to thaw‐related soil and plant changes and provides information on potential microbially mediated biogeochemical cycles in tundra ecosystems. 相似文献
7.
作者于1989年5月31日,在山西农业大学校园内毛白杨树上采集到数头僵蚜,经室内饲养,羽化出蜂,经鉴定为三叉蚜茧蜂属一新种,现记述如下。长度单位为mm。杨三叉蚜革蜂Trioxyspopuli新种(图1)雌虫:头横形,较翅基片处胸宽。头部三单眼呈锐角或近直角三角形,后颊与复眼横径近等长。复眼小,卵圆形。触角11节,第一鞭节长为基宽的3信强,与第二鞭节近等长,端鞭节细长,长为亚端鞭节的2信弱,各鞭节密生半直立长毛。胸部中胸盾片适度隆起,光滑毛稀。盾纵沟全程清晰,其边缘具毛。并胸腹节具宽大五边形小室,前中纵脊短,光滑毛稀。前翅… 相似文献
8.
The proteomic alterations of Thermoanaerobacter tengcongensis cultured at different temperatures 总被引:2,自引:0,他引:2
Wang J Zhao C Meng B Xie J Zhou C Chen X Zhao K Shao J Xue Y Xu N Ma Y Liu S 《Proteomics》2007,7(9):1409-1419
Thermoanaerobacter tengcongensis, one of many thermophilic organisms, survives harsh living conditions in temperatures ranging from 50 to 80 degrees C. In this comprehensive analysis, we present a robust approach, 2-DE and MALDI-TOF MS, to compare and identify the bacterial proteins responding to the temperature stress. In total, 164 spots of 2-DE were found with the significant changes in spot volume at three culture temperatures, 55, 75, and 80 degrees C, respectively; furthermore, 87 unique proteins were characterized by MS. Our results reveal that the electrophoretic images of the bacterial proteins, extracted from two culture temperatures (55 and 75 degrees C), had similar patterns; however, the bacteria cultured at 80 degrees C had dramatically decreased their spot volumes. Additionally, the temperature-sensitive proteins are broadly divided into two groups: specific expression at certain temperatures and consistent changes of expression responsive to temperature. For instance, three proteins closely related with redox regulation, dihydrolipoamide acyltransferase, NADH:ubiquinone oxidoreductase, and ferredoxin, were only detected in the bacteria cultured at 55 degrees C. Whereas, two chaperonins, GroES and GroEL, were found to show a consistent increase during the elevated temperatures with the determinations, either by MS or Western blot. The proteomic information, thus expedites our understanding of the molecular mechanisms regarding how thermophilic bacteria adapt to the alterations in living environment. 相似文献
9.
The dragonfly wing,which consists of veins and membrane,is of biological hierarchical material.We observed the cross-sections of longitudinal veins and membrane using Environmental Scanning Electron Microscopy (ESEM).Based on the experiments and previous studies,we described the longitudinal vein and the membrane in terms of two hierarchical levels of organization of composite materials at the micro- and nano-scales.The longitudinal vein of dragonfly wing has a complex sandwich structure with two chitinous shells and a protein layer,and it is considered as the first hierarchical level of the vein.Moreover,the chitinous shells are concentric multilayered structures.Clusters of nano-fibrils grow along the circumferential orientation embedded into the protein layer.It is considered as the second level of the hierarchy.Similarly,the upper and lower epidermises of membrane constitute the first hierarchical level of organization in micro scale.Similar to the vein shell,the membrane epidermises were found to be a paralleled multilayered structure,defined as the second hierarchical level of the membrane.Combining with the mechanical behavior analysis of the dragonfly wing,we concluded that the growth orientation of the hierarchical structure of the longitudinal vein and membrane is relevant to its biomechanical behavior. 相似文献
10.
Xu Jinying Zheng Yangyang Wang Liangjia Liu Yining Wang Xishu Li Yulin Chi Guangfan 《Cellular and molecular neurobiology》2022,42(7):2031-2053
Cellular and Molecular Neurobiology - Central nervous system injuries and diseases, such as ischemic stroke, spinal cord injury, neurodegenerative diseases, glioblastoma, multiple sclerosis, and... 相似文献