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Yangjian Cheng Xinya Xu Wenting Lin Ran Han Minghuai Liu 《Geomicrobiology journal》2016,33(10):948-953
Remediation of heavy-metal contamination by biomineralization has become an environmentally very important issue in the last two decades. Here we describe the transformation of amorphous organo-Cr(III) to chromium hydroxide oxide (guyanaite/grimaldiite) by hydrothermal treatment (HTT). First, glycine-Cr(III) was synthesized to serve as a simple model for exploring the conditions favoring HTT. Cell-bound Cr(III) was obtained by the reduction of hexavalent chromium [Cr(VI)] to trivalent chromium [Cr(III)] by Bacillus cereus. Then the reduced Cr(III) was chelated by ligands at the cell surface, forming cell-bound Cr(III). Subsequently, HTT was applied to treat cell-bound Cr(III) at different temperatures and for different lengths of time. The results showed that, by this treatment at 200°C for 7 days or at 250°C for 1 day, glycine-Cr(III) was converted to trivalent chromium mineral (guyanaite/grimaldiite), having the form of nanosheets with a length of 10~20 nm and a width of 3~5 nm under the described conditions. Cell-bound Cr(III) could also be converted to guyanaite/grimaldiite at 250°C for 9 days if it was bound by an organic compound more complex than glycine. Our finding showed that organo-Cr(III) could be transformed into minerals by an appropriate hydrothermal process, which is applicable to bioremediation of heavy-metal pollution. Our findings also suggest that organo-Cr(III) may play an important role in the biogeochemistry of chromium. 相似文献
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Rongjie Cheng Fanying Li Maolei Zhang Xin Xia Jianzhuang Wu Xinya Gao Huangkai Zhou Zhi Zhang Nunu Huang Xuesong Yang Yaliang Zhang Shunli Shen Tiebang Kang Zexian Liu Feizhe Xiao Hongwei Yao Jianbo Xu Chao Yan Nu Zhang 《Cell research》2023,33(1):30
Mutations of the RAS oncogene are found in around 30% of all human cancers yet direct targeting of RAS is still considered clinically impractical except for the KRASG12C mutant. Here we report that RAS-ON (RASON), a novel protein encoded by the long intergenic non-protein coding RNA 00673 (LINC00673), is a positive regulator of oncogenic RAS signaling. RASON is aberrantly overexpressed in pancreatic ductal adenocarcinoma (PDAC) patients, and it promotes proliferation of human PDAC cell lines in vitro and tumor growth in vivo. CRISPR/Cas9-mediated knockout of Rason in mouse embryonic fibroblasts inhibits KRAS-mediated tumor transformation. Genetic deletion of Rason abolishes oncogenic KRAS-driven pancreatic and lung cancer tumorigenesis in LSL-KrasG12D; Trp53R172H/+ mice. Mechanistically, RASON directly binds to KRASG12D/V and inhibits both intrinsic and GTPase activating protein (GAP)-mediated GTP hydrolysis, thus sustaining KRASG12D/V in the GTP-bound hyperactive state. Therapeutically, deprivation of RASON sensitizes KRAS mutant pancreatic cancer cells and patient-derived organoids to EGFR inhibitors. Our findings identify RASON as a critical regulator of oncogenic KRAS signaling and a promising therapeutic target for KRAS mutant cancers.Subject terms: Gastrointestinal cancer, Cancer therapy 相似文献
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Richard S. Brown Z. Daniel Deng Katrina V. Cook Brett D. Pflugrath Xinya Li Tao Fu Jayson J. Martinez Huidong Li Bradly A. Trumbo Martin L. Ahmann Adam G. Seaburg 《PloS one》2013,8(10)
Turbine-passed fish are exposed to rapid decreases in pressure which can cause barotrauma. The presence of an implanted telemetry tag increases the likelihood of injury or death from exposure to pressure changes, thus potentially biasing studies evaluating survival of turbine-passed fish. Therefore, a neutrally buoyant externally attached tag was developed to eliminate this bias in turbine passage studies. This new tag was designed not to add excess mass in water or take up space in the coelom, having an effective tag burden of zero with the goal of reducing pressure related biases to turbine survival studies. To determine if this new tag affects fish performance or susceptibility to predation, it was evaluated in the field relative to internally implanted acoustic transmitters (JSATS; Juvenile Salmon Acoustic Telemetry System) used widely for survival studies of juvenile salmonids. Survival and travel time through the study reach was compared between fish with either tag type in an area of high predation in the Snake and Columbia rivers, Washington. An additional group of fish affixed with neutrally-buoyant dummy external tags were implanted with passive integrated transponder (PIT) tags and recovered further downstream to assess external tag retention and injury. There were no significant differences in survival to the first detection site, 12 river kilometers (rkm) downstream of release. Travel times were also similar between groups. Conversely, externally-tagged fish had reduced survival (or elevated tag loss) to the second detection site, 65 rkm downstream. In addition, the retention study revealed that tag loss was first observed in fish recaptured approximately 9 days after release. Results suggest that this new tag may be viable for short term (<8 days) single-dam turbine-passage studies and under these situations, may alleviate the turbine passage-related bias encountered when using internal tags, however further research is needed to confirm this. 相似文献
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Heng Tai Liew Janet To Xiaohong Zhang Xinya Hemu Ning-Yu Chan Aida Serra Siu Kwan Sze Chuan-fa Liu James P. Tam 《The Journal of biological chemistry》2021,297(6)
Legumains, also known as asparaginyl endopeptidases (AEPs), cleave peptide bonds after Asn/Asp (Asx) residues. In plants, certain legumains also have ligase activity that catalyzes biosynthesis of Asx-containing cyclic peptides. An example is the biosynthesis of MCoTI-I/II, a squash family-derived cyclic trypsin inhibitor, which involves splicing to remove the N-terminal prodomain and then N-to-C-terminal cyclization of the mature domain. To identify plant legumains responsible for the maturation of these cyclic peptides, we have isolated and characterized a legumain involved in splicing, McPAL1, from Momordica cochinchinensis (Cucurbitaceae) seeds. Functional studies show that recombinantly expressed McPAL1 displays a pH-dependent, trimodal enzymatic profile. At pH 4 to 6, McPAL1 selectively catalyzed Asp-ligation and Asn-cleavage, but at pH 6.5 to 8, Asn-ligation predominated. With peptide substrates containing N-terminal Asn and C-terminal Asp, such as is found in precursors of MCoTI-I/II, McPAL1 mediates proteolysis at the Asn site and then ligation at the Asp site at pH 5 to 6. Also, McPAL1 is an unusually stable legumain that is tolerant of heat and high pH. Together, our results support that McPAL1 is a splicing legumain at acidic pH that can mediate biosynthesis of MCoTI-I/II. We purport that the high thermal and pH stability of McPAL1 could have applications for protein engineering. 相似文献
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Yuanbo Hu Chenbin Chen Xinya Tong Sian Chen Xianjing Hu Bujian Pan Xiangwei Sun Zhiyuan Chen Xinyu Shi Yingying Hu Xian Shen Xiangyang Xue Mingdong Lu 《Cell death & disease》2021,12(9)
The 5-methylcytosine (m5C) RNA methyltransferase NSUN2 is involved in the regulation of cell proliferation and metastasis formation and is upregulated in multiple cancers. However, the biological significance of NSUN2 in gastric cancer (GC) and the modification of NSUN2 itself have not been fully investigated. Here, we analyzed the expression level of NSUN2 in tissue microarrays containing 403 GC tissues by immunohistochemistry. NSUN2 was upregulated in GC, and that it was a predictor of poor prognosis. NSUN2 promotes the proliferation, migration, and invasion of GC cells in vitro. We also demonstrated that small ubiquitin-like modifier (SUMO)-2/3 interacts directly with NSUN2 by stabilizing it and mediating its nuclear transport. This facilitates the carcinogenic activity of NSUN2. Furthermore, m5C bisulfite sequencing (Bis-seq) in NSUN2-deficient GC cells showed that m5C-methylated genes are involved in multiple cancer-related signaling pathways. PIK3R1 and PCYT1A may be the target genes that participate in GC progression. Our findings revealed a novel mechanism by which NSUN2 functions in GC progression. This may provide new treatment options for GC patients.Subject terms: Gastric cancer, Post-translational modifications 相似文献
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Xinya Li Wei Li Long Chu James F. White Jr Zhi Xiong 《Journal of Plant Interactions》2016,11(1):186-192
The diversity and heavy metal (HM) tolerance of endophytic fungi (EF) associated with Dysphania ambrosioides, a hyperaccumulator from two Pb–Zn contaminated sites were investigated. A total of 237 culturable EF were isolated and identified to 43 taxa based on morphological characteristics and rDNA internal transcribed spacer analysis, of which 13 occurred as endophytes of both sites, while other taxa were only found in either site. The colonization rate, dominant genera, community structure of EF as well as the HM content in the plant from two sites were significantly different. We suggest that these differences may result from the difference in the soil HM content: lower HM content in the soil, more EF in the plant, which may enhance the plant HM accumulation and thus result higher HM in it. HM tolerance tests indicated that 50% of the isolates exhibited HM tolerance. Among them, two isolates exhibited better HM tolerance, of which FT2G59 could tolerate Pb, Zn, and Cd, and the minimum inhibitory concentration (MIC) of them were 30–50, >?680, 20–30?mmol/l, respectively. While, the isolate FT2G7 could tolerate Cd, and the MIC was 30–50?mmol/l. These isolates may have potential application in phytoremediation. 相似文献
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Nuo Zhen Xinya Wang Xiang Li Jin Xue Yitao Zhao Min Wu Dongfang Zhou Jingsheng Liu Jinshan Guo Hao Zhang 《Microbial biotechnology》2022,15(5):1324-1338
Plastics materials used for food packaging are recalcitrant, leading to a growing global environmental problem, which arouses the attention of environmental protection departments in many countries. Therefore, to meet the increasing demand for sustainable and environment-friendly consumer products, it is necessary for the food industry to develop natural antibacterial materials for food preservation. This review summarizes the common biodegradable natural antimicrobial agents and their applications in food preservation; as well as an overview of five commonly used biodegradable protein-based polymers, such as zein, soy protein isolate, gelatin and whey protein, with special emphasis on the advantages of protein-based biopolymers and their applications in food packaging industry. 相似文献
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