排序方式: 共有13条查询结果,搜索用时 156 毫秒
1.
The general morphology of the central nervous system is analysed in intact females of the predatory mite, Phytoseiulus persimilis (Acari: Phytoseiidae), using a nucleic acid label (YOYO-1) and confocal laser scanning microscopy. The somata of all cells
that comprise the synganglion reside in the cortex. The cortex harbours an estimated total of 10,000 cells. The somata are
densely packed in the cortex and cells residing in the inner cortex may only occupy about 1.8 μm. As in all Arachnida, the
synganglion is divided in a sub- and a supra-oesophageal nervous mass. Both the cortex and the neuropil appear continuous
between these two nervous masses. The sub-oesophageal nervous mass mainly consists of the four paired pedal ganglia that are
each associated with a leg. The prominent olfactory lobes are ventrally associated with the first pedal ganglia. A small opisthosomal
ganglion occupies the most caudal part of the sub-oesophageal ganglion. The rostral part of the supra-oesophageal nervous
mass consists of the paired cheliceral and palpal ganglia. The supra-oesophageal ganglion is the largest ganglion in the supra-oesophageal
nervous mass and unlike all other ganglia it is not associated with any of the major nerves. It is therefore more likely involved
in secondary information processing. 相似文献
2.
Arne Lindqvist Anneloes Mensinga Wytse Bruinsma Olaf Voets Onno Kranenburg René H Medema 《The EMBO journal》2009,28(20):3196-3206
Activation of the DNA damage checkpoint causes a cell‐cycle arrest through inhibition of cyclin‐dependent kinases (cdks). To successfully recover from the arrest, a cell should somehow be maintained in its proper cell‐cycle phase. This problem is particularly eminent when a cell arrests in G2, as cdk activity is important to establish a G2 state. Here, we identify the phosphatase Wip1 (PPM1D) as a factor that maintains a cell competent for cell‐cycle re‐entry during an ongoing DNA damage response in G2. We show that Wip1 function is required throughout the arrest, and that Wip1 acts by antagonizing p53‐dependent repression of crucial mitotic inducers, such as Cyclin B and Plk1. Our data show that the primary function of Wip1 is to retain cellular competence to divide, rather than to silence the checkpoint to promote recovery. Our findings uncover Wip1 as a first in class recovery competence gene, and suggest that the principal function of Wip1 in cellular transformation is to retain proliferative capacity in the face of oncogene‐induced stress. 相似文献
3.
Yoav Noam Markus U. Ehrengruber Annie Koh Paul Feyen Erik M. M. Manders Geoffrey W. Abbott Wytse J. Wadman Tallie Z. Baram 《The Journal of biological chemistry》2014,289(9):5889-5903
The actin-binding protein filamin A (FLNa) regulates neuronal migration during development, yet its roles in the mature brain remain largely obscure. Here, we probed the effects of FLNa on the regulation of ion channels that influence neuronal properties. We focused on the HCN1 channels that conduct Ih, a hyperpolarization-activated current crucial for shaping intrinsic neuronal properties. Whereas regulation of HCN1 channels by FLNa has been observed in melanoma cell lines, its physiological relevance to neuronal function and the underlying cellular pathways that govern this regulation remain unknown. Using a combination of mutational, pharmacological, and imaging approaches, we find here that FLNa facilitates a selective and reversible dynamin-dependent internalization of HCN1 channels in HEK293 cells. This internalization is accompanied by a redistribution of HCN1 channels on the cell surface, by accumulation of the channels in endosomal compartments, and by reduced Ih density. In hippocampal neurons, expression of a truncated dominant-negative FLNa enhances the expression of native HCN1. Furthermore, acute abrogation of HCN1-FLNa interaction in neurons, with the use of decoy peptides that mimic the FLNa-binding domain of HCN1, abolishes the punctate distribution of HCN1 channels in neuronal cell bodies, augments endogenous Ih, and enhances the rebound-response (“voltage-sag”) of the neuronal membrane to transient hyperpolarizing events. Together, these results support a major function of FLNa in modulating ion channel abundance and membrane trafficking in neurons, thereby shaping their biophysical properties and function. 相似文献
4.
The predatory mite Phytoseiulus persimilis locates its prey, the two-spotted spider mite, by means of herbivore-induced plant volatiles. The olfactory response to this
quantitatively and qualitatively variable source of information is particularly well documented. The mites perform this task
with a peripheral olfactory system that consists of just five putative olfactory sensilla that reside in a dorsal field at
the tip of their first pair of legs. The receptor cells innervate a glomerular olfactory lobe just ventral of the first pedal
ganglion. We have made a 3D reconstruction of the caudal half of the olfactory lobe in adult females. The glomerular organization
as well as the glomerular innervation appears conserved across different individuals. The adult females have, by approximation,
a 1:1 ratio of olfactory receptor cells to olfactory glomeruli. 相似文献
5.
6.
Epithelial cell migration is a complex process crucial for embryonic development, wound healing and tumor metastasis. It depends on alterations in cell–cell adhesion and integrin–extracellular matrix interactions and on actomyosin-driven, polarized leading edge protrusion. The small GTPase Rap is a known regulator of integrins and cadherins that has also been implicated in the regulation of actin and myosin, but a direct role in cell migration has not been investigated. Here, we report that activation of endogenous Rap by cAMP results in an inhibition of HGF- and TGFβ-induced epithelial cell migration in several model systems, irrespective of the presence of E-cadherin adhesion. We show that Rap activation slows the dynamics of focal adhesions and inhibits polarized membrane protrusion. Importantly, forced integrin activation by antibodies does not mimic these effects of Rap on cell motility, even though it does mimic Rap effects in short-term cell adhesion assays. From these results, we conclude that Rap inhibits epithelial cell migration, by modulating focal adhesion dynamics and leading edge activity. This extends beyond the effect of integrin affinity modulation and argues for an additional function of Rap in controlling the migration machinery of epithelial cells. 相似文献
7.
Anita Pras Bert Houben Francesco A Aprile Rene Seinstra Rodrigo Gallardo Leen Janssen Wytse Hogewerf Christian Gallrein Matthias De Vleeschouwer Alejandro MataCabana Mandy Koopman Esther Stroo Minke de Vries Samantha Louise Edwards Janine Kirstein Michele Vendruscolo Salvatore Fabio Falsone Frederic Rousseau Joost Schymkowitz Ellen A A Nollen 《The EMBO journal》2021,40(21)
While aggregation‐prone proteins are known to accelerate aging and cause age‐related diseases, the cellular mechanisms that drive their cytotoxicity remain unresolved. The orthologous proteins MOAG‐4, SERF1A, and SERF2 have recently been identified as cellular modifiers of such proteotoxicity. Using a peptide array screening approach on human amyloidogenic proteins, we found that SERF2 interacted with protein segments enriched in negatively charged and hydrophobic, aromatic amino acids. The absence of such segments, or the neutralization of the positive charge in SERF2, prevented these interactions and abolished the amyloid‐promoting activity of SERF2. In protein aggregation models in the nematode worm Caenorhabditis elegans, protein aggregation and toxicity were suppressed by mutating the endogenous locus of MOAG‐4 to neutralize charge. Our data indicate that MOAG‐4 and SERF2 drive protein aggregation and toxicity by interactions with negatively charged segments in aggregation‐prone proteins. Such charge interactions might accelerate primary nucleation of amyloid by initiating structural changes and by decreasing colloidal stability. Our study points at charge interactions between cellular modifiers and amyloidogenic proteins as potential targets for interventions to reduce age‐related protein toxicity. 相似文献
8.
Han Y Noam Y Lewis AS Gallagher JJ Wadman WJ Baram TZ Chetkovich DM 《The Journal of biological chemistry》2011,286(23):20823-20834
Ion channel trafficking and gating are often influenced by interactions with auxiliary subunits. Tetratricopeptide repeat-containing Rab8b-interacting protein (TRIP8b) is an auxiliary subunit for neuronal hyperpolarization-activated cyclic nucleotide-gated (HCN) channels. TRIP8b interacts directly with two distinct sites of HCN channel pore-forming subunits to control channel trafficking and gating. Here we use mutagenesis combined with electrophysiological studies to define and distinguish the functional importance of the HCN/TRIP8b interaction sites. Interaction with the last three amino acids of the HCN1 C terminus governed the effect of TRIP8b on channel trafficking, whereas TRIP8b interaction with the HCN1 cyclic nucleotide binding domain (CNBD) affected trafficking and gating. Biochemical studies revealed that direct interaction between TRIP8b and the HCN1 CNBD was disrupted by cAMP and that TRIP8b binding to the CNBD required an arginine residue also necessary for cAMP binding. In accord, increasing cAMP levels in cells antagonized the up-regulation of HCN1 channels mediated by a TRIP8b construct binding the CNBD exclusively. These data illustrate the distinct roles of the two TRIP8b-HCN interaction domains and suggest that TRIP8b and cAMP may directly compete for binding the HCN CNBD to control HCN channel gating, kinetics, and trafficking. 相似文献
9.
Raedt R Clinckers R Mollet L Vonck K El Tahry R Wyckhuys T De Herdt V Carrette E Wadman W Michotte Y Smolders I Boon P Meurs A 《Journal of neurochemistry》2011,117(3):461-469
Vagus nerve stimulation (VNS) is an effective adjunctive treatment for medically refractory epilepsy. In this study, we measured VNS-induced changes in hippocampal neurotransmitter levels and determined their potential involvement in the anticonvulsive action of VNS, to elucidate the mechanism of action responsible for the seizure suppressing effect of VNS in an animal model for limbic seizures. We used in vivo intracerebral microdialysis to measure VNS-induced changes in hippocampal extracellular concentrations of noradrenaline, dopamine, serotonin and GABA in freely moving, male Wistar rats. During the same experiment, the effect of VNS on pilocarpine-induced limbic seizures was assessed using video-EEG monitoring. The involvement of VNS-induced increases in hippocampal noradrenaline in the mechanims of action of VNS was evaluated by blocking hippocampal α(2)-receptors. VNS produced a significant increase in hippocampal noradrenaline concentration (69 ± 16% above baseline levels). VNS also increased the latency between pilocarpine infusion and the onset of epileptiform discharges, and reduced the duration and severity of pilocarpine-induced limbic seizures. A strong positive correlation was found between the noradrenergic and anticonvulsive effects of VNS. Blockade of hippocampal α(2 -receptors reversed the seizure-suppressing effect of VNS. VNS induces increases in extracellular hippocampal noradrenaline, which are at least partly responsible for its seizure-suppressing effect in a model for limbic seizures, and constitute a potential biomarker for the efficacy of VNS in temporal lobe epilepsy. 相似文献
10.