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Sonthisut Marisa Wongpanya Ratree Phonphoem Anan Phonphoem Wannarat Pornsiriwong 《Plant Cell, Tissue and Organ Culture》2022,148(1):167-176
Plant Cell, Tissue and Organ Culture (PCTOC) - 1-Deoxynojirimycin (DNJ), one of the main bioactive compounds in mulberry plants, is a strong inhibitor of α-glucosidase with potential... 相似文献
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Wongpanya R Boonyalai N Thammachuchourat N Horata N Arikit S Myint KM Vanavichit A Choowongkomon K 《The protein journal》2011,30(8):529-538
Betaine aldehyde dehydrogenase 2 (BADH2) is believed to be involved in the accumulation of 2-acetyl-1-pyrroline (2AP), one
of the major aromatic compounds in fragrant rice. The enzyme can oxidize ω-aminoaldehydes to the corresponding ω-amino acids.
This study was carried out to investigate the function of wild-type BADHs and four BADH2 mutants: BADH2_Y420, containing a
Y420 insertion similar to BADH2.8 in Myanmar fragrance rice, BADH2_C294A, BADH2_E260A and BADH2_N162A, consisting of a single
catalytic-residue mutation. Our results showed that the BADH2_Y420 mutant exhibited less catalytic efficiency towards γ-aminobutyraldehyde
but greater efficiency towards betaine aldehyde than wild-type. We hypothesized that this point mutation may account for the
accumulation of γ-aminobutyraldehyde/Δ1-pyrroline prior to conversion to 2AP, generating fragrance in Myanmar rice. In addition, the three catalytic-residue mutants
confirmed that residues C294, E260 and N162 were involved in the catalytic activity of BADH2 similar to those of other BADHs. 相似文献
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