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Abstract We have analyzed the sequence downstream of rpoN from Zcinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP- N -acetylgucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus . The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN . These observations confirm that A. calcoaceticus represents the first exceptin from a conserved genetic context of rpoN observed in several other Gram-negative bacteria. 相似文献
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Andrea Schneider Manuela Ruppert Oliver Hendrich Thomas Giang Maite Ogueta Stefanie Hampel Marvin Vollbach Ansgar Büschges Henrike Scholz 《PloS one》2012,7(12)
The decision to move towards a mating partner or a food source is essential for life. The mechanisms underlying these behaviors are not well understood. Here, we investigated the role of octopamine – the invertebrate analogue of noradrenaline – in innate olfactory attraction to ethanol. We confirmed that preference is caused via an olfactory stimulus by dissecting the function of the olfactory co-receptor Orco (formally known as OR83b). Orco function is not required for ethanol recognition per se, however it plays a role in context dependent recognition of ethanol. Odor-evoked ethanol preference requires the function of Tbh (Tyramine β hydroxalyse), the rate-limiting enzyme of octopamine synthesis. In addition, neuronal activity in a subset of octopaminergic neurons is necessary for olfactory ethanol preference. Notably, a specific neuronal activation pattern of tyraminergic/octopaminergic neurons elicit preference and is therefore sufficient to induce preference. In contrast, dopamine dependent increase in locomotor activity is not sufficient for olfactory ethanol preference. Consistent with the role of noradrenaline in mammalian drug induced rewards, we provide evidence that in adult Drosophila the octopaminergic neurotransmitter functions as a reinforcer and that the molecular dissection of the innate attraction to ethanol uncovers the basic properties of a response selection system. 相似文献
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G. Wolfgang Fuhs 《Archives of microbiology》1961,39(4):374-422
Ohne Zusammenfassung 相似文献
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Arthroscopic surgery causes considerably less soft tissue damage than conventional surgery. As the result of the development of an optical system employed with a chip camera and a video chain arthroscopic knee surgery can be ideally documented with excellent picture quality. Besides the advantage of soft-tissue sparing procedures, diagnosis is substantially improved and operative procedures can be kept to a minimum. This means, in particular in the case of meniscus procedures, that only the damaged areas are removed, this minimising changes to the biomechanics of the knee. The technical equipment, the arthroscopic surgery technic and the advantages of the knee arthroscopy are discussed. 相似文献
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