全文获取类型
收费全文 | 9824篇 |
免费 | 697篇 |
国内免费 | 3篇 |
出版年
2022年 | 51篇 |
2021年 | 105篇 |
2020年 | 74篇 |
2019年 | 86篇 |
2018年 | 108篇 |
2017年 | 101篇 |
2016年 | 193篇 |
2015年 | 314篇 |
2014年 | 369篇 |
2013年 | 532篇 |
2012年 | 562篇 |
2011年 | 580篇 |
2010年 | 420篇 |
2009年 | 346篇 |
2008年 | 500篇 |
2007年 | 557篇 |
2006年 | 506篇 |
2005年 | 552篇 |
2004年 | 508篇 |
2003年 | 500篇 |
2002年 | 511篇 |
2001年 | 134篇 |
2000年 | 101篇 |
1999年 | 127篇 |
1998年 | 170篇 |
1997年 | 108篇 |
1996年 | 115篇 |
1995年 | 127篇 |
1994年 | 126篇 |
1993年 | 133篇 |
1992年 | 98篇 |
1991年 | 86篇 |
1990年 | 108篇 |
1989年 | 98篇 |
1988年 | 76篇 |
1987年 | 83篇 |
1986年 | 76篇 |
1985年 | 78篇 |
1984年 | 85篇 |
1983年 | 86篇 |
1982年 | 68篇 |
1981年 | 85篇 |
1980年 | 67篇 |
1979年 | 60篇 |
1978年 | 52篇 |
1977年 | 53篇 |
1976年 | 48篇 |
1975年 | 49篇 |
1974年 | 46篇 |
1973年 | 42篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
2.
Abstract We have analyzed the sequence downstream of rpoN from Zcinetobacter calcoaceticus and identified an open reading frame encoding a protein with high similarity to UDP- N -acetylgucosamine 1-carboxyvinyl-transferase (MurZ). Multicopy plasmids encoding this enzyme conferred phosphomycin resistance to A. calcoaceticus . The polar effect of a rpoN mutation on the phosphomycin resistance level suggests that murZ is, in part, cotranscribed with rpoN . These observations confirm that A. calcoaceticus represents the first exceptin from a conserved genetic context of rpoN observed in several other Gram-negative bacteria. 相似文献
3.
Sulfur bacteria such as Beggiatoa or Thiomargarita have a particularly high capacity for storage because of their large size. In addition to sulfur and nitrate, these bacteria also store phosphorus in the form of polyphosphate. Thiomargarita namibiensis has been shown to release phosphate from internally stored polyphosphate in pulses creating steep peaks of phosphate in the sediment and thereby inducing the precipitation of phosphorus-rich minerals. Large sulfur bacteria populate sediments at the sites of recent phosphorite formation and are found as fossils in ancient phosphorite deposits. Therefore, it can be assumed that this physiology contributes to the removal of bioavailable phosphorus from the marine system and thus is important for the global phosphorus cycle. We investigated under defined laboratory conditions which parameters stimulate the decomposition of polyphosphate and the release of phosphate in a marine Beggiatoa strain. Initially, we tested phosphate release in response to anoxia and high concentrations of acetate, because acetate is described as the relevant stimulus for phosphate release in activated sludge. To our surprise, the Beggiatoa strain did not release phosphate in response to this treatment. Instead, we could clearly show that increasing sulfide concentrations and anoxia resulted in a decomposition of polyphosphate. This physiological reaction is a yet unknown mode of bacterial polyphosphate usage and provides a new explanation for high phosphate concentrations in sulfidic marine sediments. 相似文献
4.
G. Wolfgang Fuhs 《Archives of microbiology》1961,39(4):374-422
Ohne Zusammenfassung 相似文献
5.
6.
7.
8.
9.
Jake V Bailey Verena Salman Gregory W Rouse Heide N Schulz-Vogt Lisa A Levin Victoria J Orphan 《The ISME journal》2011,5(12):1926-1935
We present evidence for a dimorphic life cycle in the vacuolate sulfide-oxidizing bacteria that appears to involve the attachment of a spherical Thiomargarita-like cell to the exteriors of invertebrate integuments and other benthic substrates at methane seeps. The attached cell elongates to produce a stalk-like form before budding off spherical daughter cells resembling free-living Thiomargarita that are abundant in surrounding sulfidic seep sediments. The relationship between the attached parent cell and free-living daughter cell is reminiscent of the dimorphic life modes of the prosthecate Alphaproteobacteria, but on a grand scale, with individual elongate cells reaching nearly a millimeter in length. Abundant growth of attached Thiomargarita-like bacteria on the integuments of gastropods and other seep fauna provides not only a novel ecological niche for these giant bacteria, but also for animals that may benefit from epibiont colonization. 相似文献
10.
The overproduction of specialized metabolites requires plants to manage the inherent burdens, including the risk of self-intoxication. We present a control mechanism that stops the expression of phytoalexin biosynthetic enzymes by blocking the antecedent signal transduction cascade. Cultured cells of Eschscholzia californica (Papaveraceae) and Catharanthus roseus (Apocynaceae) overproduce benzophenanthridine alkaloids and monoterpenoid indole alkaloids, respectively, in response to microbial elicitors. In both plants, an elicitor-responsive phospholipase A2 (PLA2) at the plasma membrane generates signal molecules that initiate the induction of biosynthetic enzymes. The final alkaloids produced in the respective plant inhibit the respective PLA, a negative feedback that prevents continuous overexpression. The selective inhibition by alkaloids from the class produced in the “self” plant could be transferred to leaves of Nicotiana benthamiana via recombinant expression of PLA2. The 3D homology model of each PLA2 displays a binding pocket that specifically accommodates alkaloids of the class produced by the same plant, but not of the other class; for example, C. roseus PLA2 only accommodates C. roseus alkaloids. The interaction energies of docked alkaloids correlate with their selective inhibition of PLA2 activity. The existence in two evolutionary distant plants of phospholipases A2 that discriminate “self-made” from “foreign” alkaloids reveals molecular fingerprints left in signal enzymes during the evolution of species-specific, cytotoxic phytoalexins. 相似文献