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1.
Bharat Lagu John M. Wetzel Carlos Forray Michael A. Patane Mark G. Bock 《Bioorganic & medicinal chemistry letters》2000,10(24)
The binding affinities and selectivities of antagonists Figure 1 and Scheme 2 for the α1A-adrenoceptor are dependent on the stereochemical orientation of the groups at the C-4 and C-5 positions of the oxazolidinone ring. The unambiguous assignment of the relative and absolute configurations of the diastereomers of SNAP 7915 (Figure 1 and Scheme 2) is reported. 相似文献
2.
Daniel M. Wetzel Ursula L. Haerter Darcy B. Kelley 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1985,157(6):749-761
Vocalizations of South African clawed frogs are produced by contractions of laryngeal muscles innervated by motor neurons of the caudal medulla (within cranial nerve nucleus IX-X). We have traced afferents to laryngeal motor neurons in male and female frogs using retrograde axonal transport of horseradish peroxidase conjugated to wheat germ agglutinin (HRP-WGA). After iontophoretic injection of HRP-WGA into n. IX-X, retrogradely labelled neurons were seen in the contralateral n. IX-X, in rhombencephalic reticular nuclei, and in the pre-trigeminal nucleus of the dorsal tegmental area (DTAM) of both males and females. 相似文献
3.
Experimental Evaluation of Conversion Factors for the [3H]Thymidine Incorporation Assay of Bacterial Secondary Productivity
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The relationship between bacterial growth and incorporation of [methyl-3H]thymidine in oligotrophic lake water cultures was investigated. Prescreening, dilution, and addition of organic and inorganic nutrients were treatments used to prevent bacterivory and stimulate bacterial growth. Growth in unmanipulated samples was estimated through separate measurements of grazing losses. Both bacterial number and biovolume growth responses were measured, and incorporation of [3H]thymidine in both total macromolecules and nucleic acids was assayed. The treatments had significant effects on conversion factors used to relate thymidine incorporation to bacterial growth. Cell number-based factors ranged from 1.1 × 1018 to 38 × 1018 cells mol of total thymidine incorporation−1 and varied with treatment up to 10-fold for the same initial bacterial assemblage. In contrast, cell biovolume-based conversion factors were similar for two treatment groups across a 16-fold range of [3H]thymidine incorporation rates: 5.54 × 1017 μm3 mol of total thymidine incorporation−1 and 15.2 × 1017 μm3 mol of nucleic acid incorporation−1. Much of the variation in cell number-based conversion factors was related to changes in apparent mean cell volume of produced bacteria. Phosphorus addition stimulated [3H]thymidine incorporation more than it increased bacterial growth, which resulted in low conversion factors. 相似文献
4.
M. Klinkowski H. Lange Fabig M. Schmidt K. Wuttky J. Helm Hagemann F. Mechelke Buder Reinmuth Alfred Lein W. Laube A. Wetzel 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1960,30(6):258-264
Ohne Zusammenfassung 相似文献
5.
F. Mechelke Fabig Rieger K. Schmidt F. Scholz A. Wetzel Goerttler Hedemarie Zacharias M. Klinkowski H. Friedrich Helm Lehmann S. Danert R. Schick M. Schmiedeknecht Alfred Lein H. Rüther Nover 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1960,30(2):92-96
Ohne Zusammenfassung 相似文献
6.
A method is described for the highly selective modification of the alpha-amino groups at the N-termini of unprotected peptides to form stable, modified peptide intermediates which can be covalently coupled to other molecules or to a solid support. Acylation with iodoacetic anhydride at pH 6.0 occurs with 90-98% selectivity for the alpha-amino group, depending on the N-terminal residue (as shown with a series of model hexapeptides containing a competing Lys residue). Although Cys residues must be protected (reversibly or irreversibly) before the anhydride reaction, there are no detectable side reactions of the alpha-amino moiety--of the reagent or of modified peptide--with the side chains of His, Met, or Lys. The reaction works well in denaturants, so that inhibitory effects of noncovalent structure can be minimized. In a second step the iodoacetyl-peptide can be reacted with a thiol group on a protein, on a solid chromatography matrix, on a spectroscopic probe, etc. This is illustrated by reaction of a series of N alpha-iodoacetyl-peptides with murine interferon-gamma, which contains a C-terminal Cys residue. Data are presented which suggest that this iodoacetic anhydride scheme is superior in selectivity for alpha-amino groups to conventional chemical approaches to cross-linking such as use of 2-iminothiolane or N-hydroxysuccinimide-activated carboxylic acid esters. The reaction is ideally suited for modifying peptide fragments, as pure species or as mixtures, derived from proteolytic or chemical fragmentation of proteins. Furthermore, polypeptides synthesized biosynthetically, for example via recombinant DNA techniques, can be cross-linked in this way.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
7.
8.
Anonymous nuclear DNA markers in the American oyster and their implications for the heterozygote deficiency phenomenon in marine bivalves 总被引:4,自引:0,他引:4
A puzzling population-genetic phenomenon widely reported in allozyme
surveys of marine bivalves is the occurrence of heterozygote deficits
relative to Hardy-Weinberg expectations. Possible explanations for this
pattern are categorized with respect to whether the effects should be
confined to protein-level assays or are genomically pervasive and expected
to be registered in both protein- and DNA-level assays. Anonymous nuclear
DNA markers from the American oyster were employed to reexamine the
phenomenon. In assays based on the polymerase chain reaction (PCR), two
DNA-level processes were encountered that can lead to artifactual genotypic
scorings: (a) differential amplification of alleles at a target locus and
(b) amplification from multiple paralogous loci. We describe symptoms of
these complications and prescribe methods that should generally help to
ameliorate them. When artifactual scorings at two anonymous DNA loci in the
American oyster were corrected, Hardy-Weinberg deviations registered in
preliminary population assays decreased to nonsignificant values.
Implications of these findings for the heterozygote-deficit phenomenon in
marine bivalves, and for the general development and use of PCR-based
assays, are discussed.
相似文献
9.
Nutrient additions by waterfowl to lakes and reservoirs: predicting their effects on productivity and water quality 总被引:3,自引:3,他引:0
Lakes and reservoirs provide water for human needs and habitat for aquatic birds. Managers of such waters may ask whether nutrients added by waterfowl degrade water quality. For lakes and reservoirs where primary productivity is limited by phosphorus (P), we developed a procedure that integrates annual P loads from waterfowl and other external sources, applies a nutrient load-response model, and determines whether waterfowl that used the lake or reservoir degraded water quality. Annual P loading by waterfowl can be derived from a figure in this report, using the days per year that each kind spent on any lake or reservoir. In our example, over 6500 Canada geese (Branta canadensis) and 4200 ducks (mostly mallards, Anas platyrhynchos) added 4462 kg of carbon (C), 280 kg of nitrogen (N), and 88 kg of P y–1 to Wintergreen Lake in southwestern Michigan, mostly during their migration. These amounts were 69% of all C, 27% of all N, and 70% of all P that entered the lake from external sources. Loads from all external sources totaled 840 mg P m–2 y–1. Application of a nutrient load-response model to this concentration, the hydraulic load (0.25 m y–1), and the water residence time (9.7 y) of Wintergreen Lake yielded an average annual concentration of total P in the lake of 818 mg m–3 that classified the lake as hypertrophic. This trophic classification agreed with independent measures of primary productivity, chlorophyll-a, total P, total N, and Secchi disk transparency made in Wintergreen Lake. Our procedure showed that waterfowl caused low water quality in Wintergreen Lake.Contribution 824 of the National Fisheries Research Center-Great Lakes, 1451 Green Road, Ann Arbor, Michigan 48105, U.S.A. and 722 of the Kellogg Biological Station, Michigan State University. 相似文献
10.
MICROBIAL COLONIZATION ON NATURAL AND ARTIFICIAL MACROPHYTES IN A PHOSPHORUS-LIMITED,HARDWATER LAKE1
Epiphyte communities in a phosphorus-limited hard-water lake were compared over a 14-week period from Potamogeton illinoensis and structurally similar artificial plants of different leaf ages. Artificial plants were serially incubated in the lake to simulate the age of natural leaves. The physiognomy of loosely attached epiphytes appeared similar on the two substrata. Algal cell number and biovolume were 15-fold and 17-fold higher, respectively, on artificial leaves early in the growing season, but total algal density gradually became similar on natural and artificial plants. In contrast, the taxonomic composition of loosely attached algae became increasingly distinct, and mean cell biovolume on natural leaves was twice that on artificial leaves. Adnate epiphytes on both substrata developed from sparse populations of bacteria on new leaves to a community of diatoms, blue-green algae and numerous bacteria on mature and senescent leaves. Adnate community succession on natural leaves in late senescence/death differed from that on artificial leaves colonized for comparable periods in having (1) a marked increase in filamentous blue-green algae, (2) a subsequent decrease in all algae, and (3) a final fungi-dominated stage. The trends in colonization indicate that macrophytes in this oligotrophic lake provided a distinct habitat from that of artificial substrata for epiphytes throughout the growing season. 相似文献