全文获取类型
收费全文 | 3260篇 |
免费 | 218篇 |
国内免费 | 169篇 |
出版年
2024年 | 8篇 |
2023年 | 63篇 |
2022年 | 127篇 |
2021年 | 262篇 |
2020年 | 185篇 |
2019年 | 224篇 |
2018年 | 216篇 |
2017年 | 142篇 |
2016年 | 208篇 |
2015年 | 274篇 |
2014年 | 361篇 |
2013年 | 355篇 |
2012年 | 338篇 |
2011年 | 289篇 |
2010年 | 144篇 |
2009年 | 117篇 |
2008年 | 107篇 |
2007年 | 86篇 |
2006年 | 41篇 |
2005年 | 37篇 |
2004年 | 25篇 |
2003年 | 13篇 |
2002年 | 9篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1989年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1959年 | 2篇 |
排序方式: 共有3647条查询结果,搜索用时 22 毫秒
1.
Zhenzhen Zhang Changjiu He Yu Gao Lu Zhang Yukun Song Tianqi Zhu Kuanfeng Zhu Dongying Lv Jing Wang Xiuzhi Tian Teng Ma Pengyun Ji Wei Cui Guoshi Liu 《Aging cell》2021,20(2)
The fecundity reduction with aging is referred as the reproductive aging which comes earlier than that of chronological aging. Since humans have postponed their childbearing age, to prolong the reproductive age becomes urgent agenda for reproductive biologists. In the current study, we examined the potential associations of α‐ketoglutarate (α‐KG) and reproductive aging in mammals including mice, swine, and humans. There is a clear tendency of reduced α‐KG level with aging in the follicle fluids of human. To explore the mechanisms, mice were selected as the convenient animal model. It is observed that a long term of α‐KG administration preserves the ovarian function, the quality and quantity of oocytes as well as the telomere maintaining system in mice. α‐KG suppresses ATP synthase and alterations of the energy metabolism trigger the nutritional sensors to down‐regulate mTOR pathway. These events not only benefit the general aging process but also maintain ovarian function and delay the reproductive decline. Considering the safety of the α‐KG as a naturally occurring molecule in energy metabolism, its utility in reproduction of large mammals including humans deserves further investigation. 相似文献
2.
3.
4.
Peng Li Liang Xiao Qingzhang Du Mingyang Quan Yuepeng Song Yuling He Weixiong Huang Jianbo Xie Chenfei Lv Dan Wang Jiaxuan Zhou Lianzheng Li Qing Liu Yousry A. El-Kassaby Deqiang Zhang 《Plant biotechnology journal》2023,21(10):2002-2018
Heterozygous alleles are widespread in outcrossing and clonally propagated woody plants. The variation in heterozygosity that underlies population adaptive evolution and phenotypic variation, however, remains largely unknown. Here, we describe a de novo chromosome-level genome assembly of Populus tomentosa, an economic and ecologically important native tree in northern China. By resequencing 302 natural accessions, we determined that the South subpopulation (Pop_S) encompasses the ancestral strains of P. tomentosa, while the Northwest subpopulation (Pop_NW) and Northeast subpopulation (Pop_NE) experienced different selection pressures during population evolution, resulting in significant population differentiation and a decrease in the extent of heterozygosity. Analysis of heterozygous selective sweep regions (HSSR) suggested that selection for lower heterozygosity contributed to the local adaptation of P. tomentosa by dwindling gene expression and genetic load in the Pop_NW and Pop_NE subpopulations. Genome-wide association studies (GWAS) revealed that 88 single nucleotide polymorphisms (SNPs) within 63 genes are associated with nine wood composition traits. Among them, the selection for the homozygous AA allele in PtoARF8 is associated with reductions in cellulose and hemicellulose contents by attenuating PtoARF8 expression, and the increase in lignin content is attributable to the selection for decreases in exon heterozygosity in PtoLOX3 during adaptive evolution of natural populations. This study provides novel insights into allelic variations in heterozygosity associated with adaptive evolution of P. tomentosa in response to the local environment and identifies a series of key genes for wood component traits, thereby facilitating genomic-based breeding of important traits in perennial woody plants. 相似文献
5.
6.
7.
本文提供了男、女两性共404人ABO、MN血型各表现型的35个体质特征测量数据,对血型和测量体征的关系进行了初步讨论。统计分析表明,不同血型群体的某些单个体征平均值之间有显著差异。对所调查的35个体征作整体分析,可见男性MN系统和女性ABO、MN系统各血型群体平均值之间差异显著。在MN血型系统,MN型群体的测量体征平均值低于M型和N型与MN基因型的杂合状态有密切关系。本文还分析了4个民族的血型资料,表明MN血型在ABO血型系统是随机分布的。 相似文献
8.
Wenlong Zhang Wenpeng Yang Mingchun Wang Wei Wang Guiping Zeng Zhiwei Chen Yilin Cai 《PloS one》2013,8(2)
The low lysine content of waxy maize cannot meet the nutritional requirements of humans, livestock, or poultry. In the present study, the high-lysine genes o2 and o16 were backcrossed into wx lines using the maize high-lysine inbreds TAIXI19 (o2o2) and QCL3021 (o16o16) as donors and the waxy maize inbred line QCL5019 (wxwx) as a receptor. In the triple-cross F1, backcross, and inbred generations, the SSR markers phi027 and phi112 within the wx and o2 genes and the SSR marker umc1121 linked to the o16 gene were used for foreground selection. Background selection of the whole-genome SSR markers was performed for the selected individuals. The grain lysine content was determined using the dye-binding lysine method. The waxiness of the grain was determined with the I2-KI staining and dual-wavelength spectrophotometric analysis. The BC2F2 generation included 7 plants of genotype wxwxo2o2O16_, 19 plants of genotype wxwxo16o16O2_, and 3 plants of genotype wxwxo2o2o16o16. In these seeds, the average amylopectin content was 96.67%, 96.87%, and 96.62%, respectively, which is similar to that of QCL5019. The average lysine content was 0.555%, 0.380%, and 0.616%, respectively, representing increases of 75.1%, 19.9%, 94.3%, respectively, over QCL5019. The average genetic background recovery rate of the BC2F3 families was 95.3%, 94.3%, 94.2%, respectively. Among these 3 wxwxo2o2O16O16 families, 4 wxwxo2o2O16o16 families, and 3 wxwxo2o2o16o16 families, the longest imported parent donor fragment was 113.35 cM and the shortest fragment was 11.75 cM. No significant differences in lysine content were found between the BC2F4 seeds and the BC2F3 seeds in these 10 families. This allowed us to increase the lysine content of waxy corn and produce seeds with excellent nutritional characteristics suitable for human consumption, animal feed, and food processing. This may be of significance in the breeding of high-quality corn and in improvement of the nutrition of humans, livestock, and poultry. 相似文献
9.
10.
Luocheng Lv Zhaofu Liao Jiali Luo Hongyi Chen Hongyan Guo Jifeng Yang Ruijin Huang Qin Pu Hui Zhao Ziqiang Yuan Shanshan Feng Xufeng Qi Dongqing Cai 《Journal of cellular and molecular medicine》2020,24(4):2531-2541
Recent research has revealed that cardiac telocytes (CTs) play an important role in cardiac physiopathology and the regeneration of injured myocardium. Recently, we reported that the adult Xenopus tropicalis heart can regenerate perfectly in a nearly scar‐free manner after injury via apical resection. However, whether telocytes exist in the X tropicalis heart and are affected in the regeneration of injured X tropicalis myocardium is still unknown. The present ultrastructural and immunofluorescent double staining results clearly showed that CTs exist in the X tropicalis myocardium. CTs in the X tropicalis myocardium were mainly twined around the surface of cardiomyocyte trabeculae and linked via nanocontacts between the ends of the telopodes, forming a three‐dimensional network. CTs might play a role in the regeneration of injured myocardium. 相似文献