桂平市竹笋资源及其开发利用

桂平市食用竹笋资源丰富,有大头竹笋、吊丝竹笋、浦竹笋、毛竹笋等十余种,年产鲜笋８５００ｔ以上。原有的加工方法比较落后,现已注意引进新技术,今后还需进一步开发利用。     

乳酸乳球菌表面展示技术

乳酸乳球菌(Lactococcus lactis,L.lactis)是革兰氏阳性益生菌,作为食品安全级微生物,它在当今社会的多个方面得到运用。利用基因工程手段可以将多种外源蛋白质表达并展示在乳酸乳球菌的表面,使蛋白质的生物学功能得以展示,进而应用于工业和农业。又由于乳酸乳球菌不产生脂多糖和其他毒素,所以也能很好地运用于医学等相关研究。     

Induction of toll-like receptor 2 positive antigen-presenting cells in spleen of pristane-induced arthritis in rats

Toll-like receptors (TLRs) have been found to contribute to the pathogenesis of rheumatoid arthritis (RA). The aim of this study is to investigate the regulation and potential role of TLR2 in spleen of pristane-induced arthritis (PIA) rat, which can be used to further understand the mechanisms of RA. Arthritis in DA rats was induced by pristane. TLR2 expression in spleen was detected by real-time quantitative PCR and western blotting, and TLR2 expression at both mRNA and protein levels was upregulated in PIA rats. Peptidoglycan (PGN) was systemically administrated to PIA rats, and arthritis severity was evaluated macroscopically and microscopically. Results showed that systemic administration of PGN to PIA rats obviously deteriorated arthritis severity. TLR2 expression on splenocytes and different types of immune cells was measured by flow cytometry. And it was found that TLR2 was mainly expressed on antigen-presenting cells (APCs) of spleen, and the proportion of TLR2⁺ dendritic cells and macrophages in spleen of PIA rats was increased remarkably. Thus, we conclude that the induction of TLR2⁺ APCs in spleen may participate in the maintenance of PIA.     

基于BEPS模型的云南省碳源/汇时空特征及其适用性分析

净初级生产力(NPP)和净生态系统生产力(NEP)是估算陆地生态系统碳源/汇的重要指标,云南为我国碳汇的主要区域之一,开展云南NPP和NEP时空变化特征分析对科学评估陆地生态系统碳源/汇功能,以及开展碳排放交易具有重要意义。基于BEPS模型1981—2019年NPP和NEP产品,采用线性趋势分析、文献对比等方法,研究云南NPP和NEP时空变化特征及其在云南的适用性。结果表明：(1)1981—1999年云南NPP和NEP呈水平波动,2000年后云南NPP和NEP呈明显波动上升趋势,2000—2019年云南NPP高值区域主要分布在西部和南部,而NEP高值区则主要分布在东部和西部局部地区;(2)2000—2019年云南NPP和NEP除西北部部分地区为下降趋势外,其余大部地区为上升趋势;(3)云南NPP峰值出现在7、8月,谷值出现在2月,NEP峰值出现月份与NPP基本相同,但谷值出现月份较NPP滞后1—3个月,6—10月是云南碳汇的主要月份;(4)BEPS模型估算的NPP与目前广泛应用的CASA和遥感模型结果较为一致,时空变化特征与云南生态恢复措施和气候特征吻合,其估算的NEP与陆地生物圈模型...     

The Nogo‐B receptor promotes human hepatocellular carcinoma cell growth via the Akt signal pathway

Nogo‐B receptor (NgBR) is a type I receptor with a single transmembrane domain and specifically binds to ligand Nogo‐B. A previous study demonstrated that NgBR was highly expressed in human breast invasive ductal carcinoma and promoted epithelial‐mesenchymal transition in breast tumor cells. Our recent work found that NgBR expression was associated with a poor prognosis in human patients with hepatocellular carcinoma (HCC). Here, we elucidate that the increased expression of NgBR contributes toward the increased cell growth of human HCC cells both in vitro and in vivo. Cell viability and clonogenic survival analysis results demonstrated that knockdown of NgBR inhibits the cell growth in human HCC cells, which correlates with a reduction in the phosphorylation of Akt levels. Furthermore, overexpression of NgBR by the cotransfected pIRES‐NgBR plasmid together with NgBR siRNA in human HCC cells can rescue impaired phosphorylation of Akt levels in NgBR knockdown human HCC cells. In addition, cell viability analyses showed that NgBR overexpression can rescue the cell growth inhibition presented in human HCC NgBR knockdown cells. Taken together, our results suggest that NgBR potentially acts as an oncogene in HCC by increasing Akt activity. Thus, NgBR may represent a new potential diagnostic and therapeutic target for the treatment of HCC.     

罗汉果中甘露醇的分离和鉴定

从罗汉果的新鲜果实中提取分离出一种非三萜葡萄糖甙的甜味成分,经化学和光谱测定鉴定为D-甘露醇。     

A bicistronic expression strategy for large scale expression and purification of full-length recombinant human parathyroid hormone for osteoporosis therapy

Parathyroid hormone (PTH) contributes to the increase of trabecular connectivity and is a candidate medication for effective treating osteoporosis. PTH is a protein of 84 amino acids and some studies have suggested that the active site lies within the range from amino acid (aa) 1 to 34. However, a few reports have indicated a causal relationship between PTH (aa 1–34) and osteogenic sarcoma in rats, while some less obvious but important roles of the carboxyl-terminus of PTH were also found. Unfortunately, it is difficult to obtain the active integrated PTH (1–84) in vitro, due to the instability of both the protein and its mRNA. Because an alternative translation start site is located at +25 nucleotides downstream of the true start site, a truncated PTH can be translated. We constructed a rhPTH bicistronic expression plasmid (pTrepth) that could highly express non-fusion soluble rhPTH proteins in Escherichia coli. The BL-21(DE3) containing pTrepth was cultured on a small scale until satisfactory expression and purification results were obtained. We then amplified the transformed cells in a 15-L fermentor and harvested 27 g/L cells (wet weight). Extensive rhPTH purification was achieved by a three step chromatography process. Activity tests demonstrated that our purified protein could dramatically increase cAMP in osteosarcoma cells in vitro.     

Toll-like receptor 3 upregulation in macrophages participates in the initiation and maintenance of pristane-induced arthritis in rats

Introduction  

Toll-like receptors (TLRs) are involved in both innate and adaptive immune responses and are likely to play a complex role in the pathogenesis of human rheumatoid arthritis (RA) and experimental arthritis. The objective of this study was to identify the key TLR in pristane-induced arthritis (PIA), a rat model for RA, and to clarify its roles in the initiation and maintenance of arthritis.