Indomethacin treatment reduces microglia activation and increases numbers of neuroblasts in the subventricular zone and ischaemic striatum after focal ischaemia

Neuroblasts from the subventricular zone (SVZ) migrate to striatum following stroke, but most of them die in the ischaemic milieu and this can be related to exacerbated microglial activation. Here, we explored the effects of the non-steroidal anti-inflammatory indomethacin on microglial activation, neuronal preservation and neuroblast migration following experimental striatal stroke in adult rats. Animals were submitted to endothelin-1 (ET-1)-induced focal striatal ischaemia and were treated with indomethacin or sterile saline (i.p.) for 7 days, being perfused after 8 or 14 days. Immunohistochemistry was performed to assess neuronal loss (anti-NeuN), microglial activation (anti-Iba1, ED1) and migrating neuroblasts (anti-DCX) by counting NeuN, ED1 and DCX-positive cells in the ischaemic striatum or SVZ. Indomethacin treatment reduced microglia activation and the number of ED1⁺ cells in both 8 and 14 days post injury as compared with controls. There was an increase in the number of DCX⁺ cells in both SVZ and striatum at the same survival times. Moreover, there was a decrease in the number of NeuN⁺ cells in indomethacin-treated animals as compared with the control group at 8 days but not after 14 days post injury. Our results suggest that indomethacin treatment modulates microglia activation, contributing to increased neuroblast proliferation in the SVZ and migration to the ischaemic striatum following stroke.     

A comparison of the immediate effects of resistance, aerobic, and concurrent exercise on postexercise hypotension

A comparison of the immediate effects of resistance, aerobic, and concurrent exercise on postexercise hypotension. The influence of resistance exercise (RE), aerobic exercise (AE), and concurrent exercise (CE) on postexercise hypotension (PEH) is not known. We investigated the immediate blood pressure (BP) lowering effects of exercise after RE, AE, and CE sessions among healthy subjects. Twenty-one men (20.7 ± 0.7 years) performed 4 experimental sessions each in a within-subject design: control (CTL-seated rest for 60 minutes), RE (3 sets at 80% 1RM for 8 exercises, including upper and lower limbs), AE (7-minutes warm-up followed by 50 minutes of cycle ergometer exercise at 65% VO?peak and 3-minute cooldown), and CE (2 sets at 80% 1RM for 6 exercises among those which composed the RE session, plus 20 minutes of cycle ergometer exercise at 65% VO?peak, 7-minute warm-up and 3-minute cooldown, exactly in this order). The total duration of each exercise session was approximately 60 minutes. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were assessed by ambulatory monitoring at rest (20 minutes) and every 10 minutes after the exercise during 120 minutes while in the laboratory. The duration of the decrease in SBP was longer after AE and CE (120 minutes) compared to RE (80 minutes); and for DBP after AE (50 minutes) compared to CE (40 minutes) and RE (20 minutes) (p < 0.05). The magnitude of the decrease in SBP and DBP was similar after all exercise sessions and significantly different from CTL (p < 0.05) (SBP: RE = 4.1 ± 2.0 mm Hg, AE = 6.3 ± 1.3 mm Hg, CE = 5.1 ± 2.2 mm Hg; DBP: RE = 1.8 ± 1.1 mm Hg, AE = 1.8 ± 1.0 mm Hg, CE = 1.6 ± 0.6 mm Hg). It was concluded that exercise sessions combining aerobic and resistance activities are as effective as AE sessions and more effective than RE sessions to promote PEH.     

Niche breadth of invertebrate shredders in tropical forest streams: which taxa have restricted habitat preferences?

Despite the importance of shredders in organic matter processing in streams, information about the habitat specificities of different taxa is scarce. Herein, we evaluated the habitat preferences of invertebrate shredders in tropical forest streams. A total of 72 leaf patches were sampled in three Atlantic Forest streams (SE Brazil). For each sample, 20 environmental variables were measured, including water properties and characteristics of the leaf patch. We used an Outlying Mean Index analysis to evaluate the niche breadth and overlap of each taxon found as well as habitat preferences. In total, we found 14 shredder taxa in 68 leaf patches, and the gradient of environmental conditions influenced the distribution of most taxa. Considering taxa that occurred in atypical habitats in the study sites, Blaberidae (semiaquatic cockroaches) and Trichodactylus fluviatilis (Decapoda) showed niche segregation, while Macrobrachium potiuna (Decapoda), Tupiperla (Plecoptera), and Nectopsyche (Trichoptera) showed niche overlap. On the other hand, Heterelmis (Coleoptera), Stenochironomus (Diptera), Phylloicus major, and Triplectides gracilis (Trichoptera) occurred in typical habitats. These results showed that shredder taxa had different habitat preferences. The observed differences in niche breadths and habitat specificities among taxa probably result in different leaf processing rates across leaf patches in Atlantic Forest streams.

     

Effects of resistance training intensity, volume, and session format on the postexercise hypotensive response

The effect of resistance exercise (RE) on the postexercise systolic and diastolic blood pressure (SBP and DBP) response in young men was investigated. Group 1 (G1) and group 2 (G2) performed three 6 repetition maximum (6RM) sets in a set repetition format for 5 and 6 exercises, respectively. G1 and G2 also performed a circuit and set repetition format session, respectively, using 50% of the 6RM for 3 sets of 12 repetitions (12-repetition protocol). SBP and DBP were determined before and up to 60 minutes postexercise. G1's postexercise SBP demonstrated a significant decrease from its preexercise SBP, lasting 50 minutes after both RE sessions. G2's postexercise SBP demonstrated a significant difference from its preexercise SBP after the 6RM and 12-repetition protocol, lasting 60 and 40 minutes, respectively. The only significant difference in the DBP from rest was at 10 minutes postexercise for G2 after the 12-repetition-per-set protocol. In summary, results indicate that RE intensity affects the duration, but not the magnitude, of the postexercise hypotensive response.     

Leaf traits induce the feeding preference of a shredder of the genus Triplectides Kolenati, 1859 (Trichoptera) in an Atlantic Forest stream,Brazil: a test with native and exotic leaves

This study assessed the feeding preference of larvae of Triplectides sp. (Trichoptera, Leptoceridae) exposed to leaves of native (Hoffmannia dusenii Standley, 1931) and exotic (Eucalyptus globulus Labillardiere, 1799) trees. We hypothesized that, regardless of the origin of the leaves, larval preference is determined mainly by leaf anatomy and quality. Leaves from both species were conditioned with and without nutrient enrichment (NPK), and the four food items were offered in paired combinations to 162 larvae. Larval preference varied according to leaf combinations. In treatments containing both species, larvae preferred to feed on H. dusenii because of softer tissues and anatomical structure. The only exception was the treatment containing discs of enriched E. globulus and non-enriched H. dusenii where enhanced microbial activity on enriched leaves provided a softer resource to shredders. Our results corroborate the initial hypothesis and suggest that introduction of exotic leaves and changes in nutrient availability may affect shredder activity in streams and, consequently, organic-matter processing and ecosystem functioning.     

Cytotoxicity and genotoxicity of coronaridine from Tabernaemontana catharinensis A.DC in a human laryngeal epithelial carcinoma cell line (Hep-2)

Cancer has become a major public health problem worldwide and the number of deaths due to this disease is increasing almost exponentially. In the constant search for new treatments, natural products of plant origin have provided a variety of new compounds to be explored as antitumor agents. Tabernaemontana catharinensis is a medicinal plant that produces alkaloids with expressive antitumor activity, such as heyneanine, coronaridine and voacangine. The aim of present study was firstly to screen the cytotoxic activity of the indole alkaloids heyneanine, coronaridine and voacangine against HeLa (human cervix tumor), 3T3 (normal mouse embryo fibroblasts), Hep-2 (human laryngeal epithelial carcinoma) and B-16 (murine skin) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide); and secondly to analyze the apoptotic activity, cell membrane damage and genotoxicity of the compound that showed the best cytotoxic activity against the tumor cell lines tested. Coronaridine was the one that exhibited greater cytotoxic activity in the laryngeal carcinoma cell line Hep-2 (IC₅₀ = 54.47 μg/mL) than the other alkaloids tested (voacangine IC₅₀ = 159.33 g/mL, and heyneanine IC₅₀ = 689.45 μg/mL). Coronaridine induced apoptosis in cell lines 3T3 and Hep-2, even at high concentrations. The evaluation of genotoxicity by comet assay showed further that coronaridine caused minimal DNA damage in the Hep-2 tumor cell line, and the LDH test showed that it did not affect the plasma membrane. These results suggest that further investigation of coronaridine as an antitumor agent has merit.     

Influence of strength training on adult women's flexibility

The purpose of the current study was to investigate the effect of 10 weeks of strength training on the flexibility of sedentary middle-aged women. Twenty women were randomly assigned to either a strength training group (n = 10; age, 37 +/- 1.7 years; body mass, 65.2 +/- 10.7 kg; height, 157.7 +/- 10.8 cm; and body mass index, 25.72 +/- 3.3 kg x m(-2)) or a control group (n = 10; age, 36.9 +/- 1.2 years; body mass, 64.54 +/- 10.18 kg; height, 158.1 +/- 8.9 cm; and body mass index, 26.07 +/- 2.8 kg x m(-2)). The strength training program was a total body session performed in a circuit fashion and consisted of 7 exercises performed for 3 circuits of 8 to 12 repetitions maximum (RM), except for the abdominal exercise which was performed for 15 to 20 RM. Flexibility measurements were taken for 10 articulation movements pre and post training: shoulder flexion and extension, shoulder horizontal adduction and abduction, elbow flexion, hip flexion and extension, knee flexion, and trunk flexion and extension. Pre and post training, 10 RM strength significantly increased (p < 0.05). Of the movements examined, only shoulder horizontal adduction, hip flexion and extension, and trunk flexion and extension demonstrated significant increases (p < 0.05). Neither elbow nor knee flexion showed a significant change with weight training. The control group showed no significant change in any of the flexibility measures determined. In conclusion, weight training can increase flexibility in previously sedentary middle-aged women in some, but not all joint movements.     

Beta diversity of aquatic macroinvertebrate assemblages associated with leaf patches in neotropical montane streams

Over 70% of the total channel length in all river basins is formed by low order streams, many of which originate on mountaintops. Headwater streams play fundamental roles in processing and transporting terrestrial and aquatic organic matter, often harboring high biodiversity in bottom leaf patches deposited from riparian vegetation. The objective of this study was to assess the variation in taxonomic composition (measured by beta diversity of aquatic macroinvertebrates) among stream sites located in the Espinhaço Meridional Mountain Range, part of a UNESCO Biosphere Reserve in eastern Brazil. We tested two hypotheses. (a) Taxa turnover is the main reason for differences in aquatic insect assemblages within stream sites; we predicted that turnover would be higher than nestedness in all stream sites. (b) Stream site altitude and catchment elevation range are the main explanatory variables for the differences in beta diversity; we predicted that local stream site variables would account for only minor amounts of variation. In both dry and wet seasons, we sampled twice in two habitat types (five leaf patches in pools and five in riffles) in each of nine stream sites distributed in three different river basins. We computed average pairwise beta diversity among sampling stations and seasons in each stream site by using Jaccard and Bray–Curtis indices, and calculated the percentages of diversity resulting from turnover and nestedness. Finally, we tested the degree that local‐ or catchment‐level predictor variables explained beta diversity. We found that turnover was the main component of beta diversity and that both dissolved oxygen and elevation range best explained Bray–Curtis beta diversity. These results reinforce the importance of leaf patches in montane (sky islands) Neotropical savanna streams as biodiversity hotbeds for macroinvertebrates, and that both local and landscape variables explained beta diversity.