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Borgoyakova M. B. Karpenko L. I. Rudometov A. P. Shanshin D. V. Isaeva A. A. Nesmeyanova V. S. Volkova N. V. Belenkaya S. V. Murashkin D. E. Shcherbakov D. N. Volosnikova E. A. Starostina E. V. Orlova L. A. Danilchenko N. V. Zaikovskaya A. V. Pyankov O. V. Ilyichev A. A. 《Molecular Biology》2021,55(6):889-898
Molecular Biology - The development of preventive vaccines became the first order task in the COVID-19 pandemic caused by SARS-CoV-2. This paper reports the construction of the pVAX-RBD plasmid... 相似文献
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E. A. Volosnikova N. I. Akulova Ya. S. Gogina G. M. Levagina V. K. Mikhailova L. R. Lebedev V. F. Podgornyi Yu. V. Telegina 《Applied Biochemistry and Microbiology》2010,46(9):865-869
Optimization of the stage of fractioning of nucleotide material has been performed, and a technology for purification and
obtaining of plasmid DNAs for pharmaceutical purposes has been developed. Plasmid DNAs can be used for construction of DNA
vaccines to urgent viral infections. Conditions for extraction and preliminary purification of DNA by salt and alcohol precipitations
were determined. An effective gel-filtration procedure of separation of plasmid DNA from contaminants was suggested. 相似文献
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Esina T. I. Volosnikova E. A. Lebedev L. R. Kochneva G. V. Grazhdantseva A. A. 《Russian Journal of Bioorganic Chemistry》2020,46(3):342-348
Russian Journal of Bioorganic Chemistry - In order to develop a target drug we studied the process of conjugation of recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) and... 相似文献
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E. A. Volosnikova I. F. Demin G. M. Levagina L. R. Lebedev A. I. Zakabunin E. D. Danilenko 《Russian Journal of Bioorganic Chemistry》2016,42(6):638-645
Conjugates of recombinant human tumor necrosis factor alpha (TNFα) and alendronic acid linked through the protein sulfhydryl, carboxyl, and amino groups were obtained with crosslinking agents of different types. The conjugation reactions were conducted in solution and on a solid phase. Unlike the conjugation reactions in solution, the method involving immobilization of active components on a hydroxyapatite column was shown to result in the conjugates with a specified stoichiometry and a high degree of homogeneity. The TNFα conjugates retained the specific cytolytic activity and demonstrated the higher affinity to hydroxyapatite, an analogue of the bone mineral matrix, than TNFα. 相似文献
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