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V. V. Mozhaev N. S. Melik-nubarov M. V. Sergeeva Virginius ik nis Karel Martinek 《Biocatalysis and Biotransformation》1990,3(3):179-187
This review states that the covalent multi-point attachment of enzymes to a support is the most general approach to stabilize them against different denaturing conditions, namely against their inactivation caused by protein unfolding. It is suggested that the change in the wavelength of the maximum emission in fluorescence spectra of a protein, resulting from its denaturation, can be used to evaluate a priori the effectiveness of stabilization. The copolymerization method of enzyme immobilization, as the most promising approach to stabilizing enzymes, is discussed in detail. 相似文献
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V. V. Mozhaev N. S. Melik-nubarov Virginius ik nis Karel Martinek 《Biocatalysis and Biotransformation》1990,3(3):189-196
The molecular mechanisms of change in the thermal stability of proteins modified with low molecular weight reagents are discussed. The choice of stabilization mechanisms to be used as a general strategy for increasing enzyme stability by chemical modification is addressed. Hydrophilization of nonpolar surface areas is the most simple and reliable approach to artificial stabilization of enzymes for use in applied biochemistry and biotechnology. 相似文献
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