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1.
The goal of this study was to develop a new implantable transducer for measuring anterior cruciate ligament (ACL) graft tension postoperatively in patients who have undergone ACL reconstructive surgery. A unique approach was taken of integrating the transducer into a femoral fixation device. To devise a practical in vivo calibration protocol for the fixation device transducer (FDT), several hypotheses were investigated: (1) The use of a cable versus the actual graft as the means for applying load to the FDT during calibration has no significant effect on the accuracy of the FDT tension measurements; (2) the number of flexion angles at which the device is calibrated has no significant effect on the accuracy of the FDT measurements; (3) the friction between the graft and femoral tunnel has no significant effect on measurement accuracy. To provide data for testing these hypotheses, the FDT was first calibrated with both a cable and a graft over the full range of flexion. Then graft tension was measured simultaneously with both the FDT on the femoral side and load cells, which were connected to the graft on the tibial side, as five cadaver knees were loaded externally. Measurements were made with both standard and overdrilled tunnels. The error in the FDT tension measurements was the difference between the graft tension measured by the FDT and the load cells. Results of the statistical analyses showed that neither the means of applying the calibration load, the number of flexion angles used for calibration, nor the tunnel size had a significant effect on the accuracy of the FDT. Thus a cable may be used instead of the graft to transmit loads to the FDT during calibration, thus simplifying the procedure. Accurate calibration requires data from just three flexion angles of 0, 45, and 90 deg and a curve fit to obtain a calibration curve over a continuous range of flexion within the limits of this angle group. Since friction did not adversely affect the measurement accuracy of the FDT, the femoral tunnel can be drilled to match the diameter of the graft and does not need to be overdrilled. Following these procedures, the error in measuring graft tension with the FDT averages less than 10 percent relative to a full-scale load of 257 N. 相似文献
2.
Pirjo Rajaniemi‐Wacklin Anne Rantala Maria. A. Mugnai Silvia Turicchia Stefano Ventura Jarka Komrkov Liisa Lepist Kaarina Sivonen 《Journal of phycology》2006,42(1):226-232
In this study, the first reported isolates of the genera Snowella and Woronichinia were characterized by 16S rRNA gene sequencing and morphological analysis. Phylogenetic studies and sequences for these genera were not available previously. By botanical criteria, the five isolated strains were identified as Snowella litoralis (Häyrén) Komárek et Hindák Snowella rosea (Snow) Elenkin and Woronichinia naegeliana (Unger) Elenkin. This study underlines the identification of freshly isolated cultures, since the Snowella strains lost the colony structure and were not identifiable after extended laboratory cultivation. In the 16S rRNA gene analysis, the Snowella strains formed a monophyletic cluster, which was most closely related to the Woronichinia strain. Thus, our results show that the morphology of the genera Snowella and Woronichinia was in congruence with their phylogeny, and their phylogeny seems to support the traditional botanical classification of these genera. Furthermore, the genera Snowella and Woronichinia occurred commonly and might occasionally be the most abundant cyanobacterial taxa in mainly oligotrophic and mesotrophic Finnish lakes. Woronichinia occurred frequently and also formed blooms in eutrophic Czech reservoirs. 相似文献
3.
P. F. Ventura 《World journal of microbiology & biotechnology》1988,4(1):151-158
Summary Two major agro-industrial enterprizes have commercialized the biomethanation technology in the Philippines: the Maya Farm and the Central Azucarera de Tarlac. The Maya Farm produces biogas from the manure of 25 000 pigs. The reactors are of the batch type. The gas production is used for electricity generation, water pumping and heating, solid wastes drying etc. The Central Azucarera de Tarlac produces biogas from distillery slops and filter cake. The principle of the treatment is decantation followed by thermophilic anaerobic digestion lagooning with and without aeration and finally, irrigation of the sugar-cane fields with the final liquid effluent. The pollution load of the influent is reduced by 80% after anaerobic digestion and by 99% at the end of the treatment. The biogas produced is used to reactivate the spent carbon in the refinery kilns and in the boilers.
Resumen Dos importantes fábricas agro-industriales han comercializado la técnología de la biometanación en Filipinas: la Granja Maya y la Central Azucarera de Tarlac. La Granja Maya produce biogas a partir del estiercol de 25 000 marranos. Los reactores son de una sola fase. Elagas producido se utiliza para generar electricidad, bombear y calentar agua, secar residuos sólidos, etc. La Central Azucarera de Tarmac produce biogas a partir de desechos de destilerias y de posos de filtrado. El tratamiento consiste en una decantación seguida de una digestión termofílica anaerobia mediante encharcamiento con o sin aereación y finalmente el riego de los campos de caña de azucar con el efluente líquido resultante. El nivel de polución del afluente se reduce en un 80% después de la digestión anaerobia y en un 99% al final del tratamiento. El biogas producido se utiliza para reactivar el carbón gastado en los hornos y calderas de la refinería.
Résumé Deux importantes agro-industries ont commercialisé, la technologie de la biométhanisation aux Philippines: la ferme Maya et la sucrerie Central Azucarera de Tarlac (CAT). La ferme Maya produit du biogaz à partir du lisier de 25 000 porcs. Les digesteurs sont du type batch et la production de gaz est utilisée pour la production d'électricité, le pompage et le chauffage de l'eau, le séchage, etc. La sucrerie CAT produit du biogaz à partir de boues de distilleries et de gâteaux de filtration. Le principe du traitement est une décantation suivie d'une digestion相似文献
4.
R W Katzberg P L DiMarco T W Morris P P Harnish R F Graves P A Merguerian J A Ventura H M Proskin 《Life sciences》1988,42(20):1963-1971
This investigation examines the role of Angiotensin II in renal hemodynamic functions during acute unilateral ureteral obstruction (UUO) in a dog model. An electro magnetic flow probe was utilized to assess renal blood flow while the arteriovenous extraction technique of technetium 99m DTPA was utilized for the assessment of changes in filtration fraction and glomerular filtration rate. The effects of Angiotensin II receptor blockade on renal hemodynamic functions during acute UUO was evaluated in six dogs and compared to acute ureteral obstruction without receptor blockade in seven dogs. Angiotensin II blockade with (Sar1, Thr8)-Angiotensin II during UUO led to a striking increase in renal blood flow that was significantly different in comparison to normalized values from UUO alone (+delta 63 +/- 17 vs. +delta 22 +/- 6% at 30 min; p less than 0.05). There were, however, no significant differences in the magnitude of the decrease in filtration fraction and glomerular filtration rate in comparison to UUO alone. This investigation demonstrates that Angiotensin II has an inhibitory effect on the initial increase in renal blood flow with acute UUO. The possibility of successful pharmacologic intervention in the setting of UUO can be examined using animal models similar to the one described here. Pharmacologic treatment in the setting of acute UUO in patients might permit better preservation of renal function. 相似文献
5.
Fernando G. de Mello Jan N. Hokoç Ana L. M. Ventura Patrícia F. Gardino 《Cellular and molecular neurobiology》1991,11(5):485-496
1. Retina-cell aggregate cultures expressed glutamate decarboxylase activity (L-glutamate 1-carboxylase; EC 4.1.1.15) as a function of culture differentiation. 2. Glutamic acid decarboxylase (GAD) activity was low in the initial phases of culture and increased eight-fold until culture day 7, remaining high up to day 13 (last stage studied). 3. The addition of GABA to the culture medium 24 h after cell seeding almost totally prevented the expression of GAD activity. 4. In association with decreased enzyme activity, aggregates exposed to GABA did not display immunoreactivity for GAD, suggesting that GAD molecules were either lost from GABAergic neurons or significantly altered with GABA treatment. 5. Control, untreated aggregates showed intense GAD immunoreactivity in neurons. Positive cell bodies were characterized by a thin rim of labeled cytoplasm with thickest labeling at the emergence of the main neurite. 6. Heavily labeled patches were also observed throughout the aggregates, possibly reflecting regions enriched in neurites. 7. The GABA-mediated reduction of GAD immunoreactivity was a reversible phenomenon and could be prevented by picrotoxin. 相似文献
6.
Use of a simple method for the Epstein-Barr virus transformation of lymphocytes from members of large families of Réunion Island 总被引:2,自引:0,他引:2
A simple method for the preparation of lymphoblastoid cell lines from small amounts (100 microliter) of frozen whole blood is described. A success score greater than 90% was obtained for EBV transformations using blood samples which had been collected several months before the infection. Due to the simplicity of the technique, up to 80 samples could be processed per day. This technique was used to prepared 242 permanent cell lines from 13 large families from Réunion Island showing blood group H deficiency. These cell lines are now available for genetic studies. 相似文献
7.
Effects of N-fertilizers,straw, and dry fallow on the nitrogen balance of a flooded soil planted with rice 总被引:1,自引:0,他引:1
Teresita Santiago-Ventura Marjorie Bravo C. Daez V. Ventura I. Watanabe A. A. App 《Plant and Soil》1986,93(3):405-411
Summary Nitrogen balance studies were made on rice (Oryza sativa) grown in flooded soil in pots. A low rate of fertilizer (5.64 mg N. kg−1 soil) did not depress the N gain, but a high rate (99.72 mg N. kg−1 soil) elminated the N gain. Soil N loss was negligible since15N applied as ammonium sulfate and thoroughly mixed with the soil was recovered from the soil-plant system after 3 crops. The
observed N gain, therefore, was caused by N2-fixation, not by a reduction of soil N loss.
Straw enhanced N gain at the rate of 2–4 mg per g straw. However, this gain was not observed when soil N availability was
high. Dry fallow between rice crops decreased the N gain. 相似文献
8.
Reduced mechanical activity of perfused rat heart following morphine or enkephalin peptides administration 总被引:1,自引:0,他引:1
Carlo Clô Claudio Muscari Benedetta Tantini Carla Pignatti Pasquale Bernardi Carlo Ventura 《Life sciences》1985,37(14):1327-1333
In the isolated and perfused rat heart, the addition of morphine, methionine-enkephalin or leucine-enkephalin to the coronary perfusate, significantly reduces the mechanical activity by negatively affecting both the heart rate and the developed tension. These effects are dose dependent and maximally evident with leucine-enkephalin. Furthermore all the opioids strongly reduce the activity of isoproterenol-stimulated hearts. The suggestion is made that opioid peptides directly influence the cardiac mechanical activity possibly by interacting with membrane-receptor systems. 相似文献
9.
R. Menzel D. F. Ventura H. Hertel J. M. de Souza U. Greggers 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1986,158(2):165-177
Summary Three different methods were used to determine the spectral sensitivity of retinula cells in the compound eyes of three species of hymenopteran insects (Apis mellifera, Melipona quadrifasciata, Osmia rufa). The conventional flash method gives the least reliable results. Sensitivity is extremely sensitive to small fluctuations of the resting potential and long lasting changes induced by preceding test flashes. The ramp method, which speeds up a spectral scan to about 1 min and keeps effective illumination constant at every flash, determines S() much more reliably. The best results are obtained with the spectral scan method, which provides the experimenter with aS() function of high spectral resolution within 20 s. Using this method we demonstrate that the high observed variability inS() of individual receptors is the result of the inadequacy of the flash method, which was the only method used in earlier studies.Double microelectrode experiments and variations of the stimulus conditions reveal that field potentials and return flow of electric current produced by activated neighboring cells have no effect in the bee eye. We conclude that the model of Shaw (1975, 1981) of current flow in the locust and fly eye does not apply to the bee eye. Very rare recordings (about 1%) of UV receptors with hyperpolarizing responses to long wavelength light are interpreted as having a synaptic inhibitory connection to green receptors.The improvement of spectral measurements of single receptors allows us for the first time to model the spectral input to a color-coding network with great precision. 相似文献
10.
Assignment of orthologous relationships among mammalian alpha-globin genes by examining flanking regions reveals a rapid rate of evolution 总被引:1,自引:0,他引:1
In order to study the relationships among mammalian alpha-globin genes, we
have determined the sequence of the 3' flanking region of the human alpha 1
globin gene and have made pairwise comparisons between sequenced
alpha-globin genes. The flanking regions were examined in detail because
sequence matches in these regions could be interpreted with the least
complication from the gene duplications and conversions that have occurred
frequently in mammalian alpha-like globin gene clusters. We found good
matches between the flanking regions of human alpha 1 and rabbit alpha 1,
human psi alpha 1 and goat I alpha, human alpha 2 and goat II alpha, and
horse alpha 1 and goat II alpha. These matches were used to align the
alpha-globin genes in gene clusters from different mammals. This alignment
shows that genes at equivalent positions in the gene clusters of different
mammals can be functional or nonfunctional, depending on whether they
corrected against a functional alpha-globin gene in recent evolutionary
history. The number of alpha-globin genes (including pseudogenes) appears
to differ among species, although highly divergent pseudogenes may not have
been detected in all species examined. Although matching sequences could be
found in interspecies comparisons of the flanking regions of alpha- globin
genes, these matches are not as extensive as those found in the flanking
regions of mammalian beta-like globin genes. This observation suggests that
the noncoding sequences in the mammalian alpha-globin gene clusters are
evolving at a faster rate than those in the beta-like globin gene clusters.
The proposed faster rate of evolution fits with the poor conservation of
the genetic linkage map around alpha-globin gene clusters when compared to
that of the beta-like globin gene clusters. Analysis of the 3' flanking
regions of alpha-globin genes has revealed a conserved sequence
approximately 100-150 bp 3' to the polyadenylation site; this sequence may
be involved in the expression or regulation of alpha-globin genes.
相似文献