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1.
Oindrila Raha Biswanath Sarkar Bhaskar VKS Lakkakula Veerraju Pasumarthy Sudhakar Godi Subhankar Chowdhury Pradip Raychaudhuri Raghavendra Rao Vadlamudi 《Journal of biomedical science》2013,20(1):12
Background
Several studies have demonstrated a fundamental role for the HLA in the susceptibility of, or protection to, type 1 diabetes mellitus (T1DM). However, this has not been adequately studied in Asian Indian populations. To assess the frequency of HLA class II (DPA1, DPB1, DQA1, DQB1 and DRB1) associated to susceptibility or protection toT1DM in a Bengali population of India with diabetes.Results
Single nucleotide polymorphism study. The HLA genotyping was performed by a polymerase chain reaction followed by their HLA-DP, DQ, and DRB1 genotypes and haplotypes by sequencing method. The results are studied by Plink software. The χ2 tests were used for the inferential statistics. To our knowledge, this study is the first of a kind which has attempted to check the HLA association with T1DM by SNPs analysis. The study recruited 151 patients with T1DM and same number of ethno-linguistic, sex matched non-diabetic controls. The present study found a significant SNP rs7990 of HLA-DQA1 (p = 0.009) negative correlation, again indicating that risk from HLA is considerably more with T1DM.Conclusions
This study demonstrates that the HLA class-II alleles play a major role in genetic basis of T1DM. 相似文献2.
Blood specimens from Brahmin and Kamma caste populations of Coastal Andhra Pradesh, India are examined for A1A2BO, MNSs, and Rhesus blood groups. Predominance of A group in Brahmins and B group in Kammas is observed. Phenotype A2 records less than 4% in both populations. High frequencies of genes M, s, D, and e are exhibited among both populations. Statistically significant differences are found only for A1A2BO system between Brahmins and Kammas. 相似文献
3.
Pentyala S Ruggeri J Veerraju A Yu Z Bhatia A Desaiah D Vig P 《Indian journal of experimental biology》2010,48(7):737-743
Inositol 1,4,5-trisphosphatee (IP3), an intracellular messenger, releases Ca2+ from microsomes. Ca2+ plays a major role in regulating various cellular events like neural transmission and regulation of hormones and growth factors. Aluminum (Al), lead (Pb) and mercury (Hg) were reported to alter Ca(2+)-regulated events thereby causing neurotoxicity. Hence, an attempt was made characterize IP3 mediated Ca2+ release from rat brain microsomes under the influence of Al, Pb and Hg. Different concentrations of metals were tested over a designated time scale and their effects on IP3 mediated Ca2+ release from microsomes were monitored using Fura-2 technique. All the three metals inhibited IP3 mediated Ca2+ release, Pb being more potent. The order of potency of these three metals was Pb>Hg>Al. Except for Al, both Hg and Pb independently released Ca2+ from microsomes. Re-uptake of Ca2+ into microsomes was inhibited by all the three metals, Pb being more potent. Microsomal Ca(2+)-ATPase activity was also inhibited by all the three metals. These results suggest that neurotoxicity exerted by Al, Pb and Hg may be due to the interference of these metals with IP3 mediated calcium release and also interfering with the microsomal Ca2+ sequestration mechanism. Differential effects of heavy metal induced changes in Ca2+ flux can be used as an index of relative toxicity. 相似文献
4.
Nanoparticles of compacted DNA transfect postmitotic cells 总被引:6,自引:0,他引:6
Liu G Li D Pasumarthy MK Kowalczyk TH Gedeon CR Hyatt SL Payne JM Miller TJ Brunovskis P Fink TL Muhammad O Moen RC Hanson RW Cooper MJ 《The Journal of biological chemistry》2003,278(35):32578-32586
Charge-neutral DNA nanoparticles have been developed in which single molecules of DNA are compacted to their minimal possible size. We speculated that the small size of these DNA nanoparticles may facilitate gene transfer in postmitotic cells, permitting nuclear uptake across the 25-nm nuclear membrane pore. To determine whether DNA nanoparticles can transfect nondividing cells, growth-arrested neuroblastoma and hepatoma cells were transfected with DNA/liposome mixtures encoding luciferase. In both models, growth-arrested cells were robustly transfected by compacted DNA (6,900-360-fold more than naked DNA). To evaluate mechanisms responsible for enhanced transfection, HuH-7 cells were microinjected with naked or compacted plasmids encoding enhanced green fluorescent protein. Cytoplasmic microinjection of DNA nanoparticles generated a approximately 10-fold improvement in transgene expression as compared with naked DNA; this enhancement was reversed by the nuclear pore inhibitor, wheat germ agglutinin. To determine the upper size limit for gene transfer, DNA nanoparticles of various sizes were microinjected into the cytoplasm. A marked decrease in transgene expression was observed as the minor ellipsoidal diameter approached 25 nm. In summary, suitably sized DNA nanoparticles productively transfect growth arrested cells by traversing the nuclear membrane pore. 相似文献
5.
P Veerraju M S Babu G Jaikishan H Walter J M Naidu T V Rao B M Raju 《Anthropologischer Anzeiger; Bericht über die biologisch-anthropologische Literatur》1982,40(3):205-216
The present paper reports the distribution of blood groups and ABH saliva secretion in two Andhra tribal populations: the Koya Dora and the Konda Kammara. 100 Koya Dora and nearly 110 Konda Kammara adults of both sexes were tested for A1A2BO, MN, Rh (CcDEe) blood groups and ABH saliva secretion. The gene frequencies for A1A2BO, MN and ABH and the gene as well as chromosome frequencies for Rh (CcDEe) systems were calculated. Koya Doras show a higher incidence of A gene than B gene, while the reverse trend is seen in Konda Kammaras. Both the tribes show a high M gene frequency. No Rh(D) negative individual was found in Koya Doras, while 4.59% of Konda Kammaras are Rh(D) negative. The chromosomes CDE, CdE, cDe, cdE, Cde and cde are absent in Koya Doras, while only the four chromosomes CDE, CdE, cDe and cdE are absent in Konda Kammaras. The chromosome CDe shows the highest frequency in both the tribes. The frequency of secretors is, as usual, higher than that of nonsecretors in both the tribes. The intergroup variation between the two tribes is not statistically significant for MN, Rh (CcDEe) and ABH systems, while the difference is significant for the A1A2BO blood groups. Suitable comparisons have also been made with all the other available data from Andhra Pradesh tribal populations with respect to different systems studied. Finally Fi estimates have been calculated after Harpending et al. (1973) and Workman et al. (1974) for Koya Doras and Konda Kammaras to assess their degree of endogamy, considering the codominant systems studied, which suggest that Koya Doras are relatively more isolated than Konda Kammaras. 相似文献
6.
Four red cell enzyme polymorphisms: Esterase-D (ESD), Glyoxalase-I (GL01), Superoxide Dismutase (SOD) and Phosphoglucomutase
(PGM1 and 2), have been studied in two endogamous populations (paidi and Valmiki) of North Coastal Andhra Pradesh, South India.
Inter group comparison by chisquare analysis revealed no significant differences among them. An attempt was made to compare
with those available for other populations from Andhra Pradesh. 相似文献
7.
ABO blood groups and ABH saliva secretion were investigated in two caste populations, Reddis and Kammas of Southern Andhra Pradesh, and compared with the data of other populations. In ABO gene frequencies, the present series of Reddis and Kammas approach the values from other Andhra caste data. Moderate gene frequency of non-secretor gene in both Reddis and Kammas are noted. 相似文献
8.
Reperfusion injury is remarkable clinical issue that needs to be resolved as ischemia-reperfusion is a common phenomenon encountered in numerous clinical situations. The present communication report the involvement of nitric oxide (NO) in cardioprotection offered by flavonoids (rutin and quercetin) against myocardial ischemia reperfusion. Rutin produced better cardioprotection than quercetin in normal and diabetic rats. The observed cardioprotection offered with quercetin and rutin was partially abolished by prior administration of nitric oxide synthase inhibitor, L-NAME (N-nitro-L-arginine methyl ester) in both normal and diabetic rats. L-NAME abolished the cardioprotective actions of rutin more strongly than the cardioprotective actions of quercetin. However, mechanistic study with NOS inhibitor implied the possible partial role of nitric oxide in infarct size limiting effect of quercetin and rutin 相似文献
9.
This paper reports the sexual risk behaviour among migrant tribal community living in an eastern Indian city. The age at marriage is very low in this community and 54% of women had first intercourse before the age of 15 years with the mean of 15.8 years and it is 19.5 years for men. Pre and extramarital relations, including multi-partnered sex is prevalent. Safe sexual practices are not reported and the risk perception is very low. High prevalence of behavioral risk factors leaves a potential risk for rapid spread of human immunodeficiency virus (HIV) both in slums as well as in remote tribal areas. 相似文献
10.
Ramesh M Veerraju P 《Anthropologischer Anzeiger; Bericht über die biologisch-anthropologische Literatur》2000,58(3):269-274
Five plasma protein polymorphisms--Haptoglobin (HP), Group Specific Component (GC), Transferrin (TF), Albumin (ALB) and Caeruloplasmin (CP)--have been determined in two endogamous populations (Paidies and Valmikies) of North Coastal Andhra Pradesh, South India. The results were compared between the two populations. They revealed significant differences for the TF system only. The results were also compared with those available from other Andhra Pradesh populations. 相似文献