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1.
Levans produced by four Zymomonas mobilis strains showed antitumour activity against sarcoma 180 and Ehrlich carcinoma in Swiss albino mice. Levans from two strains (ZAP and CP4) had the highest effects. NMR analysis showed that the polymers were composed only of fructose units. The results suggested that the antineoplasic effect is associated to the polysaccharide molecular weight and that a particular molecular weight range may be responsible for this effect. 相似文献
2.
J. Y. Barbeau R. Marchal J. P. Vandecasteele 《Applied microbiology and biotechnology》1988,29(5):447-455
Summary The stability of solvent production by Clostridium acetobutylicum has been studied in continuous single-stage and two-stage fermentations. At low dilution rates, metabolic oscillations resulting from product inhibition have been observed especially in the case of fermentations controlled by product accumulation. A second type of instability also observed in product-controlled fermentations, but not in fermentations controlled by nitrogen limitation, was a long-term metabolic drift towards acid production. This acid drift has been shown to be identical to the phenomenon of culture degeneration occurring upon subculturing in batch fermentation. In addition, it was found that acid drift could be reversed by decreases in pH, temperature and dilution rate, by growth limitation in nitrogen-deficient conditions and by the addition of butyric and acetic acids. The existence of two distinct mechanisms, a short-term control (shift) and a long-term control (drift), both triggered by the same physiological conditions, is proposed in the regulation of acid and solvent production. 相似文献
3.
Isolation and characterization of butanol-resistant mutants of Clostridium acetobutylicum. 总被引:2,自引:1,他引:1
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M Hermann F Fayolle R Marchal L Podvin M Sebald J P Vandecasteele 《Applied microbiology》1985,50(5):1238-1243
In a wild-type strain of Clostridium acetobutylicum isolated from soil, solvent production appeared limited by butanol toxicity. Butanol-resistant mutants have been obtained which produced significantly higher solvent concentrations (about 30%) than the wild-type strain. Some other physiological differences were observed between a selected resistant mutant and the wild-type strain at the level of solvent resistance and sporulation. 相似文献
4.
Versieck Jacques Vanballenberghe Lidia Wittoek Ann Vermeir Gerda Vandecasteele Carlo 《Biological trace element research》1990,(1):683-689
A method is described for the determination of mercury in human blood serum and packed blood cells employing neutron activation
analysis. Great attention was devoted to the collection and manipulation of the samples. The accuracy and precision of the
method were tested by analyzing biological reference materials and by comparing the concentrations measured in a number of
serum samples to those obtained by another, independent technique (cold vapor atomic absorption spectrometry) in the same
samples. The article reports the levels measured in blood serum and packed blood cells samples from 15 adult volunteers, as
well as the figures determined in a “second-generation” biological reference material (freeze-dried human serum), prepared
and conditioned at the University of Ghent. 相似文献
5.
The microbiological fate of polycyclic aromatic hydrocarbons: carbon and oxygen balances for bacterial degradation of model compounds 总被引:3,自引:0,他引:3
M. Bouchez D. Blanchet J. -P. Vandecasteele 《Applied microbiology and biotechnology》1996,45(4):556-561
A series of pure bacterial strains belonging mainly to theRhodococcus andPseudomonas genera were grown on one of the following polycyclic aromatic hydrocarbons (PAH) supplied as sole carbon and energy source: naphthalene, fluorene, phenanthrene, anthracene, fluoranthene and pyrene. In each case, a quantitative evaluation of the carbon repartition of the PAH degraded into CO2, biomass and water-soluble metabolites was carried out. In addition, the kinetics of oxygen consumption and of water-soluble metabolite accumulation during PAH biodegradation was followed with respirometric equipment. Satisfactory carbon balances were obtained and the data correlated well with oxygen consumption values. The results show that growth on PAH presents high mineralization yields (from 56% to 77% of carbon) and sizeable production of biomass (from 16% to 35% of carbon) and limited but significant accumulation of metabolites (from 5% to 23% of carbon). The mineralization yields were higher and biomass yields lower in the case of higher PAH. Some differences between strains were also observed. 相似文献
6.
R. W. Mayes N. A. Beresford B. J. Howard C. M. Vandecasteele G. Stakelum 《Radiation and environmental biophysics》1996,35(2):101-109
Limitations of existing methods to describe the bioavailability of dietary radionuclides to ruminants (the transfer coefficient
and apparent absorption coefficient) have led to the alternative suggestion of using the true absorption coefficient (A
t). Various approaches to estimatingA
t for radiocaesium, involving the intravenous administration of a second isotope, are presented and discussed with reference
to results from studies in which a range of radiocaesium sources were examined in sheep. Although estimates ofA
t differed between the sources, they were reasonably consistent between measurement techniques. Those methods which involved
the estimation of endogenous faecal excretion of radiocaesium could be used with previously contaminated animals and did not
require continuous administrations of radiocaesium isotopes, but gave unreliable results for sources of low bioavailability.
Methods based on estimating the turnover rate of dietary radiocaesium through blood plasma were sufficiently sensitive to
measureA
t for the range of sources studied. However, they require previously uncontaminated animals and continuous administration of
both isotopes for approximately 7 days. Bioavailability is more effectively measured asA
t than as the transfer or apparent absorption coefficients sinceA
t does not incorporate factors relating to the metabolism of radiocaesium in the tissues of the animal. The results of these
studies show that differences in transfer coefficients between sheep and cattle and between sheep of differing ages are not
due to variation in absorption across the gut. The potential for applying these approaches to other radioactive elements is
discussed. 相似文献
7.
Davila Anne-Marie Marchal Rémy Vandecasteele Jean-Paul 《Journal of industrial microbiology & biotechnology》1994,13(4):249-257
Summary Sophorose lipids stand out as biosurfactants with a wide potential for industrial application and which can be produced in good yield from glucose and a lipidic cosubstrate.Candida bombicola CBS 6009 (ATCC 22214) was used in the present study. The influence of the lipidic cosubstrate on various aspects of production performance of these glycolipids (final concentration, yield) and on product composition (in particular, the structure of the hydroxy fatty acid vegetable and animal oils, markedly influenced product composition. In terms of production performance, the best substrates were oils or esters rich in C18:0 and C18:1 fatty acids. Optimal overall performance was obtained with esters (340 g L–1 sophorose lipids with rapeseed esters). Conclusions drawn from the results allow predictive evaluation of lipidic industrial substrates. 相似文献
8.
Hernandez-Perez G Fayolle F Vandecasteele JP 《Applied microbiology and biotechnology》2001,55(1):117-121
Gordonia terrae strain IFP 2001 was selected from activated sludge for its capacity to grow on ethyl t-butyl ether (ETBE) as sole carbon and energy source. ETBE was stoichiometrically degraded to t-butyl alcohol (TBA) and the activity was inducible. A constitutive strain, G. terrae IFP 2007, derived from strain IFP 2001, was also selected. Methyl t-butyl ether (MTBE) and t-amyl methyl ether (TAME) were not used as carbon and energy sources by the two strains, but cometabolic degradation of MTBE and TAME was demonstrated, to TBA and t-amyl alcohol (TAA) respectively, in the presence of a carbon source such as ethanol. No two-carbon compound was detected during growth on ETBE, but formate was produced during cometabolic degradation of MTBE or TAME. A monooxygenase was involved in the degradation of ethers, because no degradation of ETBE was observed under anaerobic conditions and the presence of a cytochrome P-450 was demonstrated in G. terrae IFP 2001 after induction by cultivation on ETBE. 相似文献
9.
Paulo FP Pimenta Alessandra S Orfano Ana C Bahia Ana PM Duarte Claudia M Ríos-Velásquez Fabrício F Melo Felipe AC Pessoa Giselle A Oliveira Keillen MM Campos Luis Martínez Villegas Nilton Barnabé Rodrigues Rafael Nacif-Pimenta Rejane C Sim?es Wuelton M Monteiro Rogerio Amino Yara M Traub-Cseko José BP Lima Maria GV Barbosa Marcus VG Lacerda Wanderli P Tadei Nágila FC Secundino 《Memórias do Instituto Oswaldo Cruz》2015,110(1):23-47
In the Americas, areas with a high risk of malaria transmission are mainly located in
the Amazon Forest, which extends across nine countries. One keystone step to
understanding the Plasmodium life cycle in Anopheles species from the Amazon Region
is to obtain experimentally infected mosquito vectors. Several attempts to colonise
Ano- pheles species have been conducted, but with only short-lived success or no
success at all. In this review, we review the literature on malaria transmission from
the perspective of its Amazon vectors. Currently, it is possible to develop
experimental Plasmodium vivax infection of the colonised and field-captured vectors
in laboratories located close to Amazonian endemic areas. We are also reviewing
studies related to the immune response to P. vivax infection of Anopheles aquasalis,
a coastal mosquito species. Finally, we discuss the importance of the modulation of
Plasmodium infection by the vector microbiota and also consider the anopheline
genomes. The establishment of experimental mosquito infections with Plasmodium
falciparum, Plasmodium yoelii and Plasmodium berghei parasites that could provide
interesting models for studying malaria in the Amazonian scenario is important.
Understanding the molecular mechanisms involved in the development of the parasites
in New World vectors is crucial in order to better determine the interaction process
and vectorial competence. 相似文献
10.
C. Block B. Van Praet T. Windels I. Vermeulen G. Dangreau A. Overmeire E. D’Hooge T. Maes G. Van Eetvelde C. Vandecasteele 《Journal of Industrial Ecology》2011,15(4):584-596
The industrial park of Herdersbrug (Brugge, Flanders, Belgium) comprises 92 small and medium‐sized enterprises, a waste‐to‐energy incinerator, and a power plant (not included in the study) on its site. To study the carbon dioxide (CO2) neutrality of the park, we made a park‐wide inventory for 2007 of the CO2 emissions due to energy consumption (electricity and fossil fuel) and waste incineration, as well as an inventory of the existing renewable electricity and heat generation. The definition of CO2 neutrality in Flanders only considers CO2 released as a consequence of consumption or generation of electricity, not the CO2 emitted when fossil fuel is consumed for heat generation. To further decrease or avoid CO2 emissions, we project and evaluate measures to increase renewable energy generation. The 21 kilotons (kt) of CO2 emitted due to electricity consumption are more than compensated by the 25 kt of CO2 avoided by generation of renewable electricity. Herdersbrug Industrial Park is thus CO2 neutral, according to the definition of the Flemish government. Only a small fraction (6.6%) of the CO2 emitted as a consequence of fossil fuel consumption (heat generation) and waste incineration is compensated by existing and projected measures for renewable heat generation. Of the total CO2 emission (149 kt) due to energy consumption (electricity + heat generation) and waste incineration on the Herdersbrug Industrial Park in 2007, 70.5% is compensated by existing and projected renewable energy generated in the park. Forty‐seven percent of the yearly avoided CO2 corresponds to renewable energy generated from waste incineration and biomass fermentation. 相似文献