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1.
Richters Valda Elliott Gary Sherwin Russell P. 《In vitro cellular & developmental biology. Plant》1978,14(5):458-464
Summary The lungs of 12 mice, half of which were exposed to continuous 0.5 ppm nitrogen dioxide for 3 weeks, were explanted in culture,
and the instances of macrophage congregation were quantitated according to numbers of target cells involved, categories of
congregation from three to 11 or more, numbers of macrophages participating in each category for the total cultures, and the
influence of delaying explantation for 24 and 96 hr. A total of 9042 macrophages and 2140 epithelial and spindle target cells
were counted in the outgrowths from 306 explants. The incidence of macrophage congregation (or numbers of target cells) was
greater for the cultures from the NO2-exposed animals, both with respect to total incidences between groups (p→0), and the 0-hr (p<0.001) and 24-hr (p<0.01) culture
subgroups. In addition, the values for T3 to T6 macrophage congregation were individually and consistently greater for the exposed animal group. Postmortem interval stress
at 96 hr appeared to result in large colonies, but they were reduced greatly in number. Also the incidence of macrophage congregation
fell by 28% as compared to 0-hr and 24-hr subgroups.
Supported by Grants NHLI No. HL 17412 and EPA No. R. 800881. 相似文献
2.
Study of the methylation and lack of deamination of deoxyribonucleic acid by N-methyl-N′-nitro-N-nitrosoguanidine 下载免费PDF全文
Valda M. Craddock 《The Biochemical journal》1969,111(5):615-620
1. DNA labelled with (14)C in the purine residues was prepared by treating newborn rats with [(14)C]formate and killing them for preparation of nucleic acids at 11-17 months. This DNA was incubated with N-methyl-N'-nitro-N-nitrosoguanidine, and then analysed for products of methylation and deamination reactions. 2. Evidence was found for the formation of 7-methylguanine and a smaller amount of 3-methyladenine, and, after preliminary denaturation of the DNA, 1-methyladenine was detected. The presence of cysteine increased the extent of methylation. No evidence was found for the formation of xanthine or hypoxanthine, even at pH5.5. 相似文献
3.
4.
Overproduction and purification of the M.HhaII methyltransferase from Haemophilus haemolyticus 总被引:4,自引:0,他引:4
The HhaII methyltransferase gene from Haemophilus haemolyticus was subcloned in an expression vector under control of the hybrid trp-lac promoter. Induction with isopropyl-beta-D-thiogalactopyranoside results in overproduction of the methyltransferase to about 3% of total cellular protein. The methyltransferase was purified to near electrophoretic homogeneity by phosphocellulose, DEAE, and gel chromatography. Its monomer Mr by sodium dodecyl sulfate-polyacrylamide gel electrophoresis is 25 kDa, in good agreement with that predicted from the nucleotide sequence. Crystals of the methyltransferase were obtained in the presence of a two-fold molar excess of the duplex oligodeoxynucleotide substrate 5'd-GGACTCC.CCTGAGG. 相似文献
5.
Stability of deoxyribonucleic acid methylated in the intact animal by administration of dimethylnitrosamine. Rate of breakdown in vivo and in vitro at different dosages 总被引:5,自引:3,他引:2 下载免费PDF全文
Valda M. Craddock 《The Biochemical journal》1969,111(4):497-502
1. The effect of administration of various dosages of dimethylnitrosamine on the extent of methylation of liver and kidney nucleic acids in the intact rat was studied. Methylation of liver nucleic acids was linearly related to the dosage, but decreasing the dose produced relatively less lowering of the extent of alkylation of kidney nucleic acids. 2. The rates of disappearance of 7-methylguanine from DNA during the 2 days after administration of dimethylnitrosamine in the intact animal and on incubation under simulated physiological conditions in vitro were compared. At a high dosage this rate was greater in vivo than in vitro. At a low dosage the small difference between the two rates was not thought to be sufficient evidence for existence of a specific enzymic excision of the abnormal base. 相似文献
6.
Effect of administration of the carcinogen dimethylnitrosamine on urinary 7-methylguanine 总被引:6,自引:6,他引:0 下载免费PDF全文
1. Evidence is presented for the excretion of 7-methylguanine in normal rat urine at a rate of approx. 65μg./day. Experiments with animals in which the nucleic acids had been prelabelled by treatment of the neonatal rats with [14C]-formate gave evidence that the methylated base originated in the nucleic acids of the rat. 2. Injection of [14C]dimethylnitrosamine leads to an increased excretion of 7-methylguanine, and the base becomes labelled in the methyl group. The disappearance of labelled 7-methylguanine formed in nucleic acids of rats treated with the carcinogen therefore does not take place by an N-demethylation reaction, but by liberation of the intact methylated base. 相似文献
7.
1. To assess the significance of the methylation of nucleic acids known to be caused by certain carcinogens, the metabolic fate of 7[14C]-methylguanine was studied, with special reference to its possible incorporation into RNA and DNA. 2. The major part (approx. 95%) of the dose was excreted unchanged in the urine. A small amount of N-demethylation took place, as evidenced by the formation of radioactive adenine and guanine, and expiration of 14C-labelled carbon dioxide. No evidence was obtained for the direct incorporation of 7-methylguanine into systems synthesizing nucleic acids, i.e. RNA in liver, DNA in intestine or in the foetus. 相似文献
8.
Stanevicha V Eglite J Sochnevs A Gardovska D Zavadska D Shantere R 《Arthritis research & therapy》2003,5(6):R340-R346
Genetic control of immune reactions has a major role in the development of rheumatic heart disease (RHD) and differs between
patients with rheumatic fever (RF). Some authors think the risk of acquiring RHD is associated with the HLA class II DR and
DQ loci, but other views exist, due to the various HLA-typing methods and ways of grouping cases. Our goal was to determine
the relations between HLA class II alleles and risk of or protection from RF in patients with relatively homogeneous clinical
manifestations. A total of 70 RF patients under the age of 18 years were surveyed in Latvia. HLA genotyping of DRB1*01 to
DRB1*18 and DQB1*0201-202, *0301-305, *0401-402, *0501-504, and *0601-608 was performed using polymerase chain reaction sequence-specific
primers. Data for a control group of 100 healthy individuals typed for HLA by the same method were available from the databank
of the Immunology Institute of Latvia. Of the RF patients, 47 had RHD and 8 had Sydenham's chorea. We concluded that HLA class
II DRB1*07-DQB1*0401-2 and DRB1*07-DQB1*0302 could be the risk alleles and HLA class II DRB1*06 and DQB1*0602-8, the protective
ones. Patients with mitral valve regurgitation more often had DRB1*07 and DQB1*0401-2, and patients with multivalvular lesions
more often had DRB1*07 and DQB1*0302. In Sydenham's chorea patients, the DQB1*0401-2 allele was more frequent. Genotyping
control showed a high risk of RF and RHD in patients with DRB1*01-DQB1*0301-DRB1*07-DQB1*0302 and DRB1*15-DQB1*0302-DRB1*07-DQB1*0303. 相似文献
9.
Joost?Swart Gabriella?Giancane Gerd?Horneff Bo?Magnusson Michael?Hofer Еkaterina?Alexeeva Violeta?Panaviene Brigitte?Bader-Meunier Jordi?Anton Susan?Nielsen Fabrizio?De Benedetti Sylvia?Kamphuis Valda?Sta?ēvi?a Maria?Tracahana Laura?Marinela?Ailioaie Elena?Tsitsami Ariane?Klein Kirsten?Minden Ivan?Foeldvari Johannes?Peter?Haas Jens?Klotsche Anna?Carin?Horne Alessandro?Consolaro Francesca?Bovis Francesca?Bagnasco Angela?Pistorio Alberto?Martini Nico?Wulffraat Nicolino?Ruperto for the Paediatric Rheumatology International Trials Organisation BiKeR the board of the Swedish Registry 《Arthritis research & therapy》2018,20(1):285
10.
Gryndler M Hršelová H Soukupová L Streiblová E Valda S Borovička J Gryndlerová H Gažo J Miko M 《FEMS microbiology letters》2011,318(1):84-91
Tuber aestivum is becoming an important commodity of great economical value in some European countries. At the same time, it is a highly protected organism in other countries, where it needs careful treatment. A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage was then used to distinguish the two species. The selectivity of the designed primer pair was evaluated using DNA extracted from specimens of a further 13 Tuber spp. Subsequently, the selectivity and robustness to false-positive results with nontarget DNA of the designed primers was compared with two other primer pairs (UncI/UncII and BTAE-F/BTAEMB-R). The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field. 相似文献