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The influence of elevated CO2 on non-structural carbohydrate distribution and fructan accumulation in wheat canopies 总被引:3,自引:1,他引:2
We grew 2.4 m2 wheat canopies in a large growth chamber under high photosynthetic photon flux (1000 μmol m−2 s−1) and using two CO2 concentrations, 360 and 1200 μmol mol−1. Photosynthetically active radiation (400–700 nm) was attenuated slightly faster through canopies grown in 360μmol mol−1 than through canopies grown in 1200μmol mol−1, even though high-CO2 canopies attained larger leaf area indices. Tissue fractions were sampled from each 5-cm layer of the canopies. Leaf tissue sampled from the tops of canopies grown in 1200μmol mol−1 accumulated significantly more total non-structural carbohydrate, starch, fructan, sucrose, and glucose (p≤ 0.05) than for canopies grown in 360μmol mol−1. Non-structural carbohydrate did not significantly increase in the lower canopy layers of the elevated CO2 treatment. Elevated CO2 induced fructan synthesis in all leaf tissue fractions, but fructan formation was greatest in the uppermost leaf area. A moderate temperature reduction of 10 °C over 5d increased starch, fructan and glucose levels in canopies grown in 1200μmol mol−1, but concentrations of sucrose and fructose decreased slightly or remained unchanged. Those results may correspond with the use of fructosyl-residues and release of glucose when sucrose is consumed in fructan synthesis. 相似文献
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Abstract Using the method of compartmental analysis, the ion fluxes and compartment concentrations of Ca2+, K+ and Cl- have been compared in the untreated vegetative frond and the abscisic acid (ABA) induced turion of Spirodela polyrrhiza. The ABA-induced turion is characterized by reduced Ca2+ exchange across the tonoplast and low vacuolar Ca2+ concentration relative to the vegetative frond. In addition the turion exhibits a higher plasmalemma flux with a correspondingly high Ca2+ concentration in the cytoplasm. The concentration of K+ and Cl- is much lower in the cytoplasm of the ABA-induced turion than in the vegetative frond with the influx/efflux ratio at both the plasmalemma and the tonoplast being less than 1, a finding exhibited also in dormant storage tissue. Treatment of vegetative fronds with ABA for 18 h resulted in a reduced K+ plasmalemma efflux relative to untreated vegetative fronds and a concomitant increase in the cytoplasmic concentration. There was no rapid effect of ABA on Ca2+, K+ or Cl- fluxes through either membrane. These results are consistent with the notion that drastic changes in ion fluxes and concentrations in the turion are a secondary consequence of ABA-induced development, possibly due to prior regulation by ABA of enzymes inherent to processes involved in membrane transport. 相似文献
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The Incidence and Serotypes of Clostridium perfringens on Beef, Pork and Lamb Carcasses 总被引:2,自引:2,他引:0
Examination by swabbing a 100 cm2 area adjacent to the anus demonstrated Clostridium perfringens on 29 of 100 beef, 66 of 100 pork and 85 of 100 lamb carcasses samples before chilling at a commercial abattoir at the end of the slaughter line. Numbers were generally low (20/100 cm2 ). Serotypes implicated in a high percentage of food poisoning outbreaks in the UK were detected at about the same frequency as others. 相似文献
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Daniel D. Murray Kazuo Suzuki Matthew Law Jonel Trebicka Jacquie Neuhaus Deborah Wentworth Margaret Johnson Michael J. Vjecha Anthony D. Kelleher Sean Emery INSIGHT ESPRIT SMART Study Groups 《PloS one》2015,10(10)
Introduction
The use of anti-retroviral therapy (ART) has dramatically reduced HIV-1 associated morbidity and mortality. However, HIV-1 infected individuals have increased rates of morbidity and mortality compared to the non-HIV-1 infected population and this appears to be related to end-organ diseases collectively referred to as Serious Non-AIDS Events (SNAEs). Circulating miRNAs are reported as promising biomarkers for a number of human disease conditions including those that constitute SNAEs. Our study sought to investigate the potential of selected miRNAs in predicting mortality in HIV-1 infected ART treated individuals.Materials and Methods
A set of miRNAs was chosen based on published associations with human disease conditions that constitute SNAEs. This case: control study compared 126 cases (individuals who died whilst on therapy), and 247 matched controls (individuals who remained alive). Cases and controls were ART treated participants of two pivotal HIV-1 trials. The relative abundance of each miRNA in serum was measured, by RTqPCR. Associations with mortality (all-cause, cardiovascular and malignancy) were assessed by logistic regression analysis. Correlations between miRNAs and CD4+ T cell count, hs-CRP, IL-6 and D-dimer were also assessed.Results
None of the selected miRNAs was associated with all-cause, cardiovascular or malignancy mortality. The levels of three miRNAs (miRs -21, -122 and -200a) correlated with IL-6 while miR-21 also correlated with D-dimer. Additionally, the abundance of miRs -31, -150 and -223, correlated with baseline CD4+ T cell count while the same three miRNAs plus miR-145 correlated with nadir CD4+ T cell count.Discussion
No associations with mortality were found with any circulating miRNA studied. These results cast doubt onto the effectiveness of circulating miRNA as early predictors of mortality or the major underlying diseases that contribute to mortality in participants treated for HIV-1 infection. 相似文献6.
LEON J. L. VAN DEN BERG PHILIPPINE VERGEER TIM C. G. RICH SIMON M. SMART DAN GUEST MIKE R. ASHMORE 《Global Change Biology》2011,17(5):1871-1883
Atmospheric nitrogen (N) deposition has been identified as a major threat to biodiversity, but field surveys of its effects have rarely focussed on sites which are actively managed to maintain characteristic species. We analysed permanent quadrat data from 106 plots in nature reserves on calcareous grassland sites in the United Kingdom collected during a survey between 1990 and 1993 and compared the data with the results from resurvey of 48 of these plots between 2006 and 2009. N deposition showed no significant spatial association with species richness, species diversity, or the frequency of species adapted to low nutrient conditions in the 1990–1993 dataset. However, temporal analysis showed that N deposition was significantly associated with changes in Shannon diversity and evenness. In plots with high rates of N deposition, there was a decrease in species diversity and evenness, a decline in the frequency of characteristic calcareous grassland species, and a lower number of rare and scarce species. As all sites had active management to maintain a high diversity and characteristic species, our results imply that even focussed management on nature conservation objectives cannot prevent adverse effects of high rates of N deposition. Structural equation modelling was used to compare different causal mechanisms to explain the observed effects. For change in Shannon diversity, direct effects of N deposition were the dominant mechanism and there was an independent effect of change in grazing intensity. In contrast, for change in herb species number, indirect effects on soil acidity, linked to both N and S deposition, were more important than direct effects of N deposition. 相似文献
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Mobilization of sediment phosphorus by submersed freshwater macrophytes 总被引:10,自引:0,他引:10
SUMMARY. The mobilization of sediment phosphorus (P) by three submersed freshwater macrophyte species was investigated on five different sediments. The study was conducted under controlled environmental conditions in lucite columns that enabled the separation of sediment and plant roots from the overlying P-free 'complete' nutrient solution. The species investigated ( Egeria densa, Hydrilla verticillata , and Myriophyllum spicatum ) had minor root systems (on a biomass basis), but were demonstrated to be fully capable of deriving their P nutrition exclusively from the sediments. Phosphorus absorption and translocation into shoots (i.e., mobilization) was substantial, and in some cases suggested a greater than 1000-fold turnover of interstitial water PO4 -P over a 3-month period. Sediment P mobilization, a function of both plant growth and tissue P concentration, differed considerably among plant species and sediments. Phosphorus release from the species investigated appears to be primarily dependent upon tissue decay rather than excretory processes. The mobilization of sediment P by submersed macrophytes represents an important aspect of the P cycle, and may affect the overall metabolism of lacustrine systems. 相似文献
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Abstract The developmental process leading to the formation of the abscisic acid (ABA) induced turion of Spirodela polyrrhiza was accompanied by a repression of nucleic acid and protein synthesis. DNA synthesis in the developing lurion (induced by 10−4mol m−3 ABA) was inhibited within 3h of ABA addition, followed by a repression of protein synthesis after 24 h, while RNA synthesis was not inhibited until 3 d. The inhibitory effect of ABA on protein synthesis was found to be selective and the synthesis of several novel proteins appeared to be induced. These effects were specific to ABA-sensitive tissue. The relationship between the changes in the protein and mRNA profiles during the development of the turion was investigated. The rapid general inhibition of protein synthesis at early stages of lurion formation could not be accounted for by the level of translatable mRNA, indicating an effect of ABA at the translational level. The specific alteration to the pattern of in vivo labelled proteins could have resulted, however, from control of the level of specific mRNAs for those particular proteins. Only after 3 d in ABA, when the developing primordium is committed to the turion developmental pathway, is there a total inhibition in the production of mRNA leading to the shutdown of all primary processes and the onset of the irreversible events leading to the dormant state. 相似文献
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Transverse hydraulic redistribution by a grapevine 总被引:4,自引:0,他引:4
Root hydraulic redistribution has been shown to occur in numerous plant species under both field and laboratory conditions. To date, such water redistribution has been demonstrated in two fundamental ways, either lifting water from deep edaphic sources to dry surface soils or redistributing water downward (reverse flow) when inverted soil Ψs gradients exist. The importance of hydraulic redistribution is not well documented in agricultural ecosystems under field conditions, and would be important because water availability can be temporally and spatially constrained. Herein we report that a North American grapevine hybrid (Vitis riparia × V. berlandieri cv 420 A) growing in an agricultural ecosystem can redistribute water from a restricted zone of available water under a drip irrigation emitter, laterally across the high resistance pathways of the trunk and into roots and soils on the non-irrigated side. Deuterium-labelled water was used to demonstrate lateral movement across the vine's trunk and reverse flow into roots. Water redistribution from the zone of available water and into roots distant from the source occurred within a relatively short time frame of 36 h, although overnight deposition into rhizosphere soils around the roots was not detected. Deuterium was eventually detected in rhizosphere soils adjacent to roots on the non-irrigated side after 7 d. Application of identical amounts of water with the same deuterium enrichment level (2%) to soils without grapevine roots showed that physical transport of water through the vapour phase could not account for either downward or transverse movement of the label. These results confirmed that root presence facilitated the transport of label into soils distant from the wetted zone. When deuterium-labelled water was allowed to flow directly into the trunk above the root–trunk interface, reverse flow occurred and lateral movement across the trunk and into roots originating around the collar region did not encounter large disproportionate resistances. Rapid redistribution of water into the entire root system may have important implications for woody perennial cultivars growing where water availability is spatially heterogeneous. Under the predominantly dry soil conditions studied in this investigation, water redistributed into roots may extend root longevity and increase the vines water capacitance during periods of high transpiration demand. These benefits would be enhanced by diminished water loss from roots, and could be equally important to other cited benefits of hydraulic redistribution into soils such as enhancement of nutrient acquisition. 相似文献