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1.
V. P. Upelniek A. Yu. Novoselskaya J. Sutka G. Galiba E. V. Metakovsky 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1995,90(3-4):372-379
Electrophoretic patterns of seed storage proteins, the high-molecular-weight glutenins and gliadins, were studied in 468 plants of the common wheat cultivar Chinese Spring regenerated from callus culture of immature embryos, in 115 plants grown from seeds treated with nitrosoethylurea and in 260 control plants. From 5 to 21 single grains were analysed from each plant. In these three groups, the frequency of inherited mutations causing the loss of all proteins controlled by a locus (null-mutations, probably caused by a chromosomal deficiency) was 0.69%, 2.07%, and 0.05% per locus (the differences were statistically significant), respectively, while that of mutations causing the loss of a single protein band was 0.11%, 0.33%, and 0.05%, respectively. The loss of all of the gliadins controlled by Gli-B1 or GH-B2 (mutations were probably caused by a deletion of satellites of the corresponding chromosomes), was significantly higher than the loss of gliadins controlled by genomes A and D. Gene mutations altering the electrophoretic mobility of a single protein band in the pattern were found only in the second group of plants (0.44%). Therefore, chemical mutagenesis which produced not only more mutations than cultivation of immature wheat embryos in vitro, but also a higher ratio of mutations that altered DNA sequences, can be considered as an easier and comparatively more promising way for obtaining new improved variants of loci controlling biochemical characteristics in wheat. Somaclonal variation, on the other hand, was probably mainly caused by chromosomal abnormalities and could therefore hardly be considered as a useful tool in wheat breeding. 相似文献
2.
AV Shevchenko IG Budzanivska TP Shevchenko VP Polischuk D Spaar 《Archives Of Phytopathology And Plant Protection》2013,46(2):139-146
The work was focused on the investigation of possible dependencies between the development of viral infection in plants and the presence of high heavy metal concentrations in soil. Field experiments have been conducted in order to study the development of systemic tobacco mosaic virus (TMV) infection in Lycopersicon esculentum L. cv. Miliana plants under effect of separate salts of heavy metals Cu, Zn and Pb deposited in soil. As it is shown, simultaneous effect of viral infection and heavy metals in tenfold maximum permissible concentration leads to decrease of total chlorophyll content in experiment plants mainly due to the degradation of chlorophyll a. The reduction of chlorophyll concentration under the combined influence of both stress factors was more serious comparing to the separate effect of every single factor. Plants' treatment with toxic concentrations of lead and zinc leaded to slight delay in the development of systemic TMV infection together with more than twofold increase of virus content in plants that may be an evidence of synergism between these heavy metal's and virus' effects. Contrary, copper although decreased total chlorophyll content but showed protective properties and significantly reduced amount of virus in plants. 相似文献
3.
Fabrizio Grandi Marcia M Colodel Lidianne N Monteiro João Rafael VP Leão Noeme S Rocha 《BMC veterinary research》2010,6(1):45
Backgroud
Extramedullary hematopoiesis (EMH) is defined as the presence of hematopoietic stem cells such as erythroid and myeloid lineage plus megakaryocytes in extramedullary sites like liver, spleen and lymph nodes and is usually associated with either bone marrow or hematological disorders. Mammary EMH is a rare condition either in human and veterinary medicine and can be associated with benign mixed mammary tumors, similarly to that described in this case. 相似文献4.
Recombination mapping of some chromosome 1A-, 1B-, 1D- and 6B-controlled gliadins and low-molecular-weight glutenin subunits in common wheat 总被引:2,自引:0,他引:2
E. V. Metakovsky G. Branlard V. M. Chernakov V. P. Upelniek R. Redaelli N. E. Pogna 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(6-7):788-795
Inheritance of low-molecular-weight glutenin subunits (LMW GS) and gliadins was studied in the segregating progeny from several
crosses between common wheat genotypes. The occurrence of a few recombinants in the F2 grains of the cross Skorospelka Uluchshennaya×Kharkovskaya 6 could be accounted for by assuming that the short arm of chromosome
1D contains two tightly linked loci each coding for at least one gliadin plus one C-type LMW GS. These loci were found to
recombine at a frequency of about 2%, and to be linked to the Glu-D3 locus coding for B-type LMW GS. Some proteins showing biochemical characteristics of D-type or C-type LMW GS were found to
be encoded by the Gli-B1 and Gli-B2 loci, respectively. Strongly stained B-type LMW GS in cvs Skorospelka Uluchshennaya and Richelle were assigned to the Glu-B3 locus, but recombination between this locus and Gli-B1 was not found. Analogously, in the cross Bezostaya 1×Anda, no recombination was found between Gli-A1 and Glu-A3, suggesting the maximum genetic distance between these loci to be 0.97% (P=0.05). A B-type LMW GS in cv Kharkovskaya 6 was assigned to the Glu-B2 locus, with about 25% recombination from the Gli-B1 locus. The present results suggested that alleles at Gli loci may relate to dough quality and serve as genetic markers of certain LMW GS affecting breadmaking quality.
Received: 9 July 1996/Accepted: 15 November 1996 相似文献
5.
Syringa species: molecular marking of species and cultivars 总被引:1,自引:0,他引:1
Kochneva EZ Ryzhova NN Molkanova OI Kudriavtsev AM Upelniek VP Okuneva IB 《Genetika》2004,40(1):37-40
RAPD analysis was carried out with 22 accessions of the genus Syringa, including six species, one interspecific hybrid, and 15 cultivars. In total, 500 polymorphic fragments were detected; species-specific and cultivar-specific markers were identified. For the first time, genetic polymorphism and genome similarity coefficients were estimated and phylogenetic relationships were established for the genus Syringa. 相似文献
6.
V. P. Upelniek T. A. Brezhneva S. Ya. Dadashev O. A. Novozhilova O. I. Molkanova V. F. Semikhov 《Russian Journal of Genetics》2003,39(12):1426-1431
Prolamine proteolysis is assumed to be among numerous adaptability factors in cereals. The patterns of gliadin proteolysis have been studied in 16 cultivars of spring wheat via analysis of electrophoretic spectra. Four proteolytic patterns have been identified. It is hypothesized that the cultivars characterized by early and rapid proteolysis (the first and third types) are the most adaptable. The gliadin genetic formulas of chromosomes of the first homeologous group have been determined. The alleles of gliadin loci (Gli-A1f, Gli-B1e, Gli-D1a, and Gli-D1b) have been found that can be used as markers of adaptability in spring wheat cultivars. 相似文献
7.
N. V. Melnikova E. V. Borhert S. P. Martynov I. B. Okuneva O. I. Molkanova V. P. Upelniek A. M. Kudryavtsev 《Russian Journal of Genetics》2009,45(1):85-90
RAPD analysis was used to verify the varieties in an in vitro germplasm collection of lilac Syringa vulgaris L. RAPD patterns were obtained with 16 decanucleotide primers for 46 accessions (microclones and corresponding reference varieties). The RAPD patterns of a microclone and the corresponding reference variety often differed in composition; consequently, it was infeasible to verify the accessions by direct comparison of the RAPD patterns. Hence, evaluation of the relative genetic distances between accessions (microclones) and known varieties was proposed as a method to verify lilac in vitro germplasm collections. 相似文献
8.
Upelniek VP Brezhneva TA Dadashev SIa Novozhilova OA Molkanova OI Semikhov VF 《Genetika》2003,39(12):1680-1686
Prolamine proteolysis is assumed to be among numerous adaptability factors in cereals. The patterns of gliadin proteolysis have been studied in 16 cultivars of spring wheat via analysis of electrophoretic spectra. Four proteolytic patterns have been identified. It is hypothesized that the cultivars characterized by early and rapid proteolysis (the first and third types) are the most adaptable. The gliadin genetic formulas of chromosomes of the first homeologous group have been determined. The alleles of gliadin loci (Gli-Alf, Gli-Ble, Gli-D1a, and Gli-D1b) have been found that can be used as markers of adaptability in spring wheat cultivars. 相似文献
9.
V Geetha Das Moumita Zarei Mehrdad VP Mayookha Harohally Nanishankar V G Suresh Kumar 《Glycoconjugate journal》2022,39(4):525-542
Glycoconjugate Journal - Glycosaminoglycans (GAGs) are bioactive polysaccharides or glycoconjugates found in the fish waste having significant health impacts. In the present study it has been... 相似文献
10.
Genetic control of gliadin components in wheat <Emphasis Type="Italic">Triticum spelta</Emphasis> L.
The componental composition of electrophoretic spectra of gliadin in Triticum spelta L. was studied. By analogy with common wheat T. aestivum L., it was established that genes controlling gliadin components in spelt are also located in short arms of chromosomes of
homeological groups 1 and 6. Analysis of gliadin spectra in F2 grains from the crosses k-20539 × Ershovskaya 32 and k-20558 × Ershovskaya 32 revealed linkage of some components and their
grouping into blocks (alleles) of coinherited gliadin components. Alleles of gliadin-coding loci identical to alleles of common
wheat and new alleles earlier unknown for wheat populations have been identified. 相似文献